|
Journal of Lipid Research, Vol. 41, 1689-1694, October 2000
Copyright © 2000 by Lipid Research, Inc.
Eicosanoids as endogenous regulators of leptin release and lipolysis by mouse adipose tissue in primary culture
John N. Faina,
Charles W. Lefflerb, and
Suleiman W. Bahouthc
a Departments of Biochemistry, College of Medicine, University of Tennessee-Memphis, Memphis, TN 38163
b Physiology, College of Medicine, University of Tennessee-Memphis, Memphis, TN 38163
c Pharmacology, College of Medicine, University of Tennessee-Memphis, Memphis, TN 38163
Correspondence to:
John N. Fain
Prostaglandin E2 (PGE2) stimulated leptin release over a 24-h incubation of mouse adipose tissue in primary culture. The maximal stimulation of leptin release was seen with 100 nM PGE2. The role of endogenous eicosanoids in the regulation of lipolysis and leptin formation was examined in the presence of NS-398, a selective cyclooxygenase-2 inhibitor. NS-398 at a concentration of 5 µM enhanced lipolysis by 30% and lowered leptin release by 24%. This concentration of NS-398 almost completely inhibited PGE2 formation. An inhibition of basal lipolysis by PGE2 or N6-cyclopentyladenosine (CPA) was seen in the presence but not in the absence of NS-398. CPA, whose receptor, like that of PGE2 inhibits cyclic AMP accumulation in adipose tissue, also enhanced leptin release.
These data indicate that PGE2 can stimulate leptin release and suggest that endogenous eicosanoids affect both lipolysis and leptin formation by mouse adipose tissue. Fain, J. N., C. W. Leffler, and S. W. Bahouth. Eicosanoids as endogenous regulators of leptin release and lipolysis by mouse adipose tissue in primary culture. J. Lipid Res. 2000. 41: 1689;1694.
Supplementary key words:
dexamethasone, NS-398, cyclooxygenase-2, PGE2, CPA

CiteULike Complore Connotea Del.icio.us Digg Reddit Technorati What's this?
This article has been cited by other articles:

|
 |

|
 |
 
R. E. Duncan, E. Sarkadi-Nagy, K. Jaworski, M. Ahmadian, and H. S. Sul
Identification and Functional Characterization of Adipose-specific Phospholipase A2 (AdPLA)
J. Biol. Chem.,
September 12, 2008;
283(37):
25428 - 25436.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
H. Gogas, M. Trakatelli, N. Dessypris, A. Terzidis, A. Katsambas, G. P. Chrousos, and E. T. Petridou
Melanoma risk in association with serum leptin levels and lifestyle parameters: a case-control study
Ann. Onc.,
February 1, 2008;
19(2):
384 - 389.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Sanchez-Alavez, I. Klein, S. E. Brownell, I. V. Tabarean, C. N. Davis, B. Conti, and T. Bartfai
Night eating and obesity in the EP3R-deficient mouse
PNAS,
February 20, 2007;
104(8):
3009 - 3014.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Bullo, M. R. Peeraully, and P. Trayhurn
Stimulation of NGF expression and secretion in 3T3-L1 adipocytes by prostaglandins PGD2, PGJ2, and {Delta}12-PGJ2
Am J Physiol Endocrinol Metab,
July 1, 2005;
289(1):
E62 - E67.
[Abstract]
[Full Text]
[PDF]
|
 |
|
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.
|
Advertisement
Advertisement
|