HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fain, J. N. Articles by Bahouth, S. W. Search for Related Content PubMed PubMed Citation Articles by Fain, J. N. Articles by Bahouth, S. W. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 41, 1689-1694, October 2000
Copyright © 2000 by Lipid Research, Inc.

Original Article

Eicosanoids as endogenous regulators of leptin release and lipolysis by mouse adipose tissue in primary culture

Correspondence to: John N. Fain

Prostaglandin E₂ (PGE₂) stimulated leptin release over a 24-h incubation of mouse adipose tissue in primary culture. The maximal stimulation of leptin release was seen with 100 nM PGE₂. The role of endogenous eicosanoids in the regulation of lipolysis and leptin formation was examined in the presence of NS-398, a selective cyclooxygenase-2 inhibitor. NS-398 at a concentration of 5 µM enhanced lipolysis by 30% and lowered leptin release by 24%. This concentration of NS-398 almost completely inhibited PGE₂ formation. An inhibition of basal lipolysis by PGE₂ or N⁶-cyclopentyladenosine (CPA) was seen in the presence but not in the absence of NS-398. CPA, whose receptor, like that of PGE₂ inhibits cyclic AMP accumulation in adipose tissue, also enhanced leptin release.

These data indicate that PGE₂ can stimulate leptin release and suggest that endogenous eicosanoids affect both lipolysis and leptin formation by mouse adipose tissue. — Fain, J. N., C. W. Leffler, and S. W. Bahouth. Eicosanoids as endogenous regulators of leptin release and lipolysis by mouse adipose tissue in primary culture. J. Lipid Res. 2000. 41: 1689;–1694.

Supplementary key words: dexamethasone, NS-398, cyclooxygenase-2, PGE₂, CPA

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				H. Gogas, M. Trakatelli, N. Dessypris, A. Terzidis, A. Katsambas, G. P. Chrousos, and E. T. Petridou Melanoma risk in association with serum leptin levels and lifestyle parameters: a case-control study Ann. Onc., February 1, 2008; 19(2): 384 - 389. [Abstract] [Full Text] [PDF]

				M. Sanchez-Alavez, I. Klein, S. E. Brownell, I. V. Tabarean, C. N. Davis, B. Conti, and T. Bartfai Night eating and obesity in the EP3R-deficient mouse PNAS, February 20, 2007; 104(8): 3009 - 3014. [Abstract] [Full Text] [PDF]

				M. Bullo, M. R. Peeraully, and P. Trayhurn Stimulation of NGF expression and secretion in 3T3-L1 adipocytes by prostaglandins PGD2, PGJ2, and {Delta}12-PGJ2 Am J Physiol Endocrinol Metab, July 1, 2005; 289(1): E62 - E67. [Abstract] [Full Text] [PDF]