HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cartwright, I. J. Articles by Higgins, J. A. Search for Related Content PubMed PubMed Citation Articles by Cartwright, I. J. Articles by Higgins, J. A. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 41, 1728-1739, November 2000
Copyright © 2000 by Lipid Research, Inc.

Original Article

Intracellular events in the assembly of chylomicrons in rabbit enterocytes

Correspondence to: Joan A. Higgins

The aim of this study was to determine the intracellular events in chylomicron assembly in adult villus enterocytes. We have used novel methods for separation of the intracellular components of the secretory compartment [rough and smooth endoplasmic reticulum (RER and SER, respectively) and Golgi], and their membrane and luminal components, from villus enterocytes isolated from rabbit small intestine. The steady state composition of the components of the secretory compartment and the intracellular pools of newly synthesized apolipoprotein B-48 (apoB-48) and triacylglycerol (TAG) was determined. The observations indicate that the SER is the main site of TAG synthesis and of chylomicron assembly. Newly synthesized apoB-48 and TAG accumulate in the SER membrane and are transferred into the lumen in a microsomal triglyceride transfer protein-dependent step. In enterocytes isolated from chow-fed rabbits, in which fat absorption is relatively slow, transfer of apoB-48 and TAG from the SER membrane into the lumen appears to be rate limiting. In enterocytes from fat-fed rabbits, TAG accumulates in the lumen of the SER, suggesting that movement out of the SER lumen becomes rate limiting, when chylomicron secretion is markedly stimulated. In these cells, the cytosolic TAG also increased to 450 µg/g enterocytes, compared with 12 µg/g enterocytes from chow-fed rabbits, indicating that transfer of TAG from the SER membrane into the secretory pathway can become saturated, so that newly synthesized TAG moves into the cytosol. — Cartwright, I. J., D. Plonné, and J. A. Higgins. Intracellular events in the assembly of chylomicrons in rabbit enterocytes. J. Lipid Res. 2000. 41: 1728;–1739.

Supplementary key words: RER, SER, Golgi, iodixanol, cell fractionation, fat absorption, triacylglycerol, apolipoprotein B, MTP

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. Zhu, B. Lee, K. K. Buhman, and J.-X. Cheng A dynamic, cytoplasmic triacylglycerol pool in enterocytes revealed by ex vivo and in vivo coherent anti-Stokes Raman scattering imaging J. Lipid Res., June 1, 2009; 50(6): 1080 - 1089. [Abstract] [Full Text] [PDF]

				S. Ghoshal, J. Witta, J. Zhong, W. de Villiers, and E. Eckhardt Chylomicrons promote intestinal absorption of lipopolysaccharides J. Lipid Res., January 1, 2009; 50(1): 90 - 97. [Abstract] [Full Text] [PDF]

				J. Storch, Y. X. Zhou, and W. S. Lagakos Metabolism of apical versus basolateral sn-2-monoacylglycerol and fatty acids in rodent small intestine J. Lipid Res., August 1, 2008; 49(8): 1762 - 1769. [Abstract] [Full Text] [PDF]

				E. Morel, S. Demignot, D. Chateau, J. Chambaz, M. Rousset, and F. Delers Lipid-dependent Bidirectional Traffic of Apolipoprotein B in Polarized Enterocytes Mol. Biol. Cell, January 1, 2004; 15(1): 132 - 141. [Abstract] [Full Text] [PDF]

				Y. Xie, F. Nassir, J. Luo, K. Buhman, and N. O. Davidson Intestinal lipoprotein assembly in apobec-1-/- mice reveals subtle alterations in triglyceride secretion coupled with a shift to larger lipoproteins Am J Physiol Gastrointest Liver Physiol, October 1, 2003; 285(4): G735 - G746. [Abstract] [Full Text] [PDF]

				D. Y. Hui and P. N. Howles Carboxyl ester lipase: structure-function relationship and physiological role in lipoprotein metabolism and atherosclerosis J. Lipid Res., December 1, 2002; 43(12): 2017 - 2030. [Abstract] [Full Text] [PDF]

				S. Lu, Y. Yao, S. Meng, X. Cheng, and D. D. Black Overexpression of Apolipoprotein A-IV Enhances Lipid Transport in Newborn Swine Intestinal Epithelial Cells J. Biol. Chem., August 23, 2002; 277(35): 31929 - 31937. [Abstract] [Full Text] [PDF]

				S. Lu, M. Huffman, Y. Yao, C. M. Mansbach II, X. Cheng, S. Meng, and D. D. Black Regulation of MTP expression in developing swine J. Lipid Res., August 1, 2002; 43(8): 1303 - 1311. [Abstract] [Full Text] [PDF]