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Journal of Lipid Research, Vol. 41, 1912-1920, December 2000
Copyright © 2000 by Lipid Research, Inc.

Original Article

Effects of overexpression of the amino-terminal fragment of apolipoprotein B on apolipoprotein B and lipoprotein production

Correspondence to: Ira J. Goldberg

In vitro studies have shown that the binding site for microsomal triglyceride transfer protein (MTP) is within the first 17% of apoB (apoB-17). Expression of apoB-48 in McArdle cells decreases endogenous lipoprotein production; however, overexpression of human apoB in transgenic mice does not decrease endogenous mouse apoB expression. To assess this inconsistency, adenoviruses expressing human apoB-17 (AdB17) or apoB-17-ß (which contains apoB-17 plus a small lipid-binding ß-sheet region of apoB, AdB-17ß) were produced. Hepatoma cells were infected with AdB17 or AdB17-ß with AdLacZ, an adenovirus expressing ß-galactosidase, as a control. Overexpression of apoB-17 and apoB-17-ß in hepatoma cells to levels 2- to 3-fold greater than that of endogenous apoB did not alter endogenous apoB production. This was also true in the presence of oleic acid and N-acetyl-leucyl-leucyl-norleucinal. High levels of apoB-17 or ß-galactosidase expression reduced apoB-100 production; however, control protein production was also reduced. To assess the effects of apoB-17 expression in vivo, mice of three different strains were injected with AdB17. Two days after injection, plasma apoB-17 was approximately 24 times the amount of endogenous apoB in the C57BL/6 mice, 2 times the apoB-100 in human apoB transgenic mice, and 4 times the apoB-48 in apoE knockout mice. Overexpression of apoB-17 did not decrease apoB-100 or apoB-48 concentrations in mouse plasma as assessed by Western blot analysis.

These results demonstrate that although the apoB-17 binds to MTP in vitro, it does not alter endogenous apoB expression in mice or in hepatoma cells. — Li, Z., Y. Kako, L. Pang, M. W. Freeman, J. M. Glick, X. Wang, and I. J. Goldberg. Effects of overexpression of the amino-terminal fragment of apolipoprotein B on apolipoprotein B and lipoprotein production. J. Lipid Res. 2000. 41: 1912;–1920.

Supplementary key words: adenovirus, microsomal triglyceride transfer protein, lipoprotein lipase, very low density lipoproteins, chylomicrons

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