HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fu, Y. Articles by Lopes-Virella, M. F. Search for Related Content PubMed PubMed Citation Articles by Fu, Y. Articles by Lopes-Virella, M. F. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 41, 2017-2023, December 2000
Copyright © 2000 by Lipid Research, Inc.

Original Article

Oxidized LDL induces the expression of ALBP/aP2 mRNA and protein in human THP-1 macrophages

Correspondence to: Yuchang Fu

The adipocyte lipid-binding protein (ALBP/aP2) belongs to a multigene family of fatty acid and retinoid transport proteins. This protein is abundantly expressed in the cytoplasm and nuclear region of adipocytes and is postulated to serve as a lipid shuttle, solubilizing hydrophobic fatty acids and delivering them to the appropriate metabolic system for utilization. This report demonstrates that human cholesterol-loaded THP-1 macrophages express ALBP/aP2 and that its expression can be stimulated by oxidized low density lipoprotein (oxLDL). The increase in mRNA expression was paralleled by a similar increase in ALBP/aP2 protein. The increase in ALBP/aP2 mRNA and protein in oxLDL-stimulated THP-1 macrophages is concentration and time dependent and is inhibited by treatment of the cells with an antioxidant inhibitor of nuclear factor-B (NF-B), pyrrolidine dithiocarbamate (PDTC), and with protein kinase C (PKC) inhibitors bisindolylmaleimide I and Ro-31-8220.

These results suggest that activation of both NF-B and PKC signaling pathways is necessary for oxLDL-induced ALBP/aP2 gene expression in THP-1 macrophages and that the upregulation of the fatty acid carrier may be a necessary event in foam cell formation. — Fu, Y., N. Luo, and M. F. Lopes-Virella. Oxidized LDL induces the expression of ALBP/aP2 mRNA and protein in human THP-1 macrophages. J. Lipid Res. 2000. 41: 2017;–2023.

Supplementary key words: oxidized low density lipoprotein, signaling pathways, atherosclerosis

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				H. Elmasri, C. Karaaslan, Y. Teper, E. Ghelfi, M. Weng, T. A. Ince, H. Kozakewich, J. Bischoff, and S. Cataltepe Fatty acid binding protein 4 is a target of VEGF and a regulator of cell proliferation in endothelial cells FASEB J, November 1, 2009; 23(11): 3865 - 3873. [Abstract] [Full Text] [PDF]

				D. C-L. Lam, A. Xu, K. S-L. Lam, B. Lam, J. C-M. Lam, M. M-S. Lui, and M. S-M. Ip Serum adipocyte-fatty acid binding protein level is elevated in severe OSA and correlates with insulin resistance Eur. Respir. J., February 1, 2009; 33(2): 346 - 351. [Abstract] [Full Text] [PDF]

				D. C.Y. Yeung, A. Xu, A. W.K. Tso, W.S. Chow, N. M.S. Wat, C. H.Y. Fong, S. Tam, P. C. Sham, and K. S.L. Lam Circulating Levels of Adipocyte and Epidermal Fatty Acid-Binding Proteins in Relation to Nephropathy Staging and Macrovascular Complications in Type 2 Diabetic Patients Diabetes Care, January 1, 2009; 32(1): 132 - 134. [Abstract] [Full Text] [PDF]

				D.C.Y. Yeung, A. Xu, C.W.S. Cheung, N.M.S. Wat, M.H. Yau, C.H.Y. Fong, M.T. Chau, and K.S.L. Lam Serum Adipocyte Fatty Acid-Binding Protein Levels Were Independently Associated With Carotid Atherosclerosis Arterioscler Thromb Vasc Biol, August 1, 2007; 27(8): 1796 - 1802. [Abstract] [Full Text] [PDF]

				A. T. Shamshiev, F. Ampenberger, B. Ernst, L. Rohrer, B. J. Marsland, and M. Kopf Dyslipidemia inhibits Toll-like receptor-induced activation of CD8{alpha}-negative dendritic cells and protective Th1 type immunity J. Exp. Med., February 19, 2007; 204(2): 441 - 452. [Abstract] [Full Text] [PDF]

				M. S. Rolph, T. R. Young, B. O. V. Shum, C. Z. Gorgun, C. Schmitz-Peiffer, I. A. Ramshaw, G. S. Hotamisligil, and C. R. Mackay Regulation of Dendritic Cell Function and T Cell Priming by the Fatty Acid-Binding Protein aP2 J. Immunol., December 1, 2006; 177(11): 7794 - 7801. [Abstract] [Full Text] [PDF]

				A. Munteanu, M. Taddei, I. Tamburini, E. Bergamini, A. Azzi, and J.-M. Zingg Antagonistic Effects of Oxidized Low Density Lipoprotein and {alpha}-Tocopherol on CD36 Scavenger Receptor Expression in Monocytes: INVOLVEMENT OF PROTEIN KINASE B AND PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR-{gamma} J. Biol. Chem., March 10, 2006; 281(10): 6489 - 6497. [Abstract] [Full Text] [PDF]

				A. Xu, Y. Wang, J. Y. Xu, D. Stejskal, S. Tam, J. Zhang, N. M.S. Wat, W. K. Wong, and K. S.L. Lam Adipocyte Fatty Acid-Binding Protein Is a Plasma Biomarker Closely Associated with Obesity and Metabolic Syndrome Clin. Chem., March 1, 2006; 52(3): 405 - 413. [Abstract] [Full Text] [PDF]

				A. Majdalawieh, L. Zhang, I. V. Fuki, D. J. Rader, and H.-S. Ro Adipocyte enhancer-binding protein 1 is a potential novel atherogenic factor involved in macrophage cholesterol homeostasis and inflammation PNAS, February 14, 2006; 103(7): 2346 - 2351. [Abstract] [Full Text] [PDF]

				J. Tian, K. Ishibashi, K. Ishibashi, K. Reiser, R. Grebe, S. Biswal, P. Gehlbach, and J. T. Handa Advanced glycation endproduct-induced aging of the retinal pigment epithelium and choroid: A comprehensive transcriptional response PNAS, August 16, 2005; 102(33): 11846 - 11851. [Abstract] [Full Text] [PDF]

				M. R. Kazemi, C. M. McDonald, J. K. Shigenaga, C. Grunfeld, and K. R. Feingold Adipocyte Fatty Acid-Binding Protein Expression and Lipid Accumulation Are Increased During Activation of Murine Macrophages by Toll-Like Receptor Agonists Arterioscler Thromb Vasc Biol, June 1, 2005; 25(6): 1220 - 1224. [Abstract] [Full Text] [PDF]

				L. Makowski, K. C. Brittingham, J. M. Reynolds, J. Suttles, and G. S. Hotamisligil The Fatty Acid-binding Protein, aP2, Coordinates Macrophage Cholesterol Trafficking and Inflammatory Activity: MACROPHAGE EXPRESSION OF aP2 IMPACTS PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR {gamma} AND I{kappa}B KINASE ACTIVITIES J. Biol. Chem., April 1, 2005; 280(13): 12888 - 12895. [Abstract] [Full Text] [PDF]

				E. M. Fach, L.-A. Garulacan, J. Gao, Q. Xiao, S. M. Storm, Y. P. Dubaquie, S. A. Hefta, and G. J. Opiteck In Vitro Biomarker Discovery for Atherosclerosis by Proteomics Mol. Cell. Proteomics, December 1, 2004; 3(12): 1200 - 1210. [Abstract] [Full Text] [PDF]

				J. B. Boord, K. Maeda, L. Makowski, V. R. Babaev, S. Fazio, M. F. Linton, and G. S. Hotamisligil Combined Adipocyte-Macrophage Fatty Acid-Binding Protein Deficiency Improves Metabolism, Atherosclerosis, and Survival in Apolipoprotein E-Deficient Mice Circulation, September 14, 2004; 110(11): 1492 - 1498. [Abstract] [Full Text] [PDF]

				C. Whatling, H. Bjork, S. Gredmark, A. Hamsten, and P. Eriksson Effect of macrophage differentiation and exposure to mildly oxidized LDL on the proteolytic repertoire of THP-1 monocytes J. Lipid Res., September 1, 2004; 45(9): 1768 - 1776. [Abstract] [Full Text] [PDF]

				L. Makowski and G. S. Hotamisligil Fatty Acid Binding Proteins--The Evolutionary Crossroads of Inflammatory and Metabolic Responses J. Nutr., September 1, 2004; 134(9): 2464S - 2468S. [Abstract] [Full Text] [PDF]

				L. Sun, A. C. Nicholson, D. P. Hajjar, A. M. Gotto Jr., and J. Han Adipogenic differentiating agents regulate expression of fatty acid binding protein and CD36 in the J744 macrophage cell line J. Lipid Res., October 1, 2003; 44(10): 1877 - 1886. [Abstract] [Full Text] [PDF]

				H. Scholz, A. Yndestad, J. K. Damas, T. Waehre, S. Tonstad, P. Aukrust, and B. Halvorsen 8-Isoprostane increases expression of interleukin-8 in human macrophages through activation of mitogen-activated protein kinases Cardiovasc Res, October 1, 2003; 59(4): 945 - 954. [Abstract] [Full Text] [PDF]

				S.-R. Han, A. Momeni, K. Strach, P. Suriyaphol, D. Fenske, K. Paprotka, S. I. Hashimoto, M. Torzewski, S. Bhakdi, and M. Husmann Enzymatically Modified LDL Induces Cathepsin H in Human Monocytes: Potential Relevance in Early Atherogenesis Arterioscler Thromb Vasc Biol, April 1, 2003; 23(4): 661 - 667. [Abstract] [Full Text] [PDF]

				J. B. Boord, K. Maeda, L. Makowski, V. R. Babaev, S. Fazio, M. F. Linton, and G. S. Hotamisligil Adipocyte Fatty Acid-Binding Protein, aP2, Alters Late Atherosclerotic Lesion Formation in Severe Hypercholesterolemia Arterioscler Thromb Vasc Biol, October 1, 2002; 22(10): 1686 - 1691. [Abstract] [Full Text] [PDF]

				H. Okazaki, J.-i. Osuga, K. Tsukamoto, N. Isoo, T. Kitamine, Y. Tamura, S. Tomita, M. Sekiya, N. Yahagi, Y. Iizuka, et al. Elimination of Cholesterol Ester from Macrophage Foam Cells by Adenovirus-mediated Gene Transfer of Hormone-sensitive Lipase J. Biol. Chem., August 23, 2002; 277(35): 31893 - 31899. [Abstract] [Full Text] [PDF]

				J. Han, D. P. Hajjar, X. Zhou, A. M. Gotto Jr., and A. C. Nicholson Regulation of Peroxisome Proliferator-activated Receptor-gamma -mediated Gene Expression. A NEW MECHANISM OF ACTION FOR HIGH DENSITY LIPOPROTEIN J. Biol. Chem., June 21, 2002; 277(26): 23582 - 23586. [Abstract] [Full Text] [PDF]