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Journal of Lipid Research, Vol. 41, 277-284, February 2000
Copyright © 2000 by Lipid Research, Inc.

Original Article

Regulation of calcium signalling by docosahexaenoic acid in human T-cells: implication of CRAC channels

Correspondence to: Naim Akhtar Khan

We elucidated the role of docosahexaenoic acid (DHA) on the increases in free intracellular calcium concentrations, [Ca²⁺]i, in human (Jurkat) T-cell lines. DHA evoked an increase in [Ca²⁺]i in a dose-dependent manner in these cells. Anti-CD3 antibody, known to stimulate increases in Ca²⁺ from endoplasmic reticulum (ER) via the production of inositol trisphosphate, also evoked increases in [Ca²⁺]i in Jurkat T-cells. We also used thapsigargin which inhibits Ca²⁺-ATPase of the ER and, therefore, increases Ca²⁺ in the cytosol. Interestingly, addition of DHA during the thapsigargin-induced peak response exerted an additive effect on the increases in [Ca²⁺]i in human T-cells, indicating that the mechanisms of action of these two agents are different. However, the DHA-induced calcium response was not observed when this agent was added during the anti-CD3-induced calcium peak, though its addition resulted in a prolonged and sustained calcium response as a function of time, suggesting that DHA recruits calcium, in part, from the ER pool and the prolonged response may be due to Ca²⁺ influx. In the medium containing 0% Ca²⁺, the DHA-evoked response on the increases in [Ca²⁺]i was significantly curtailed as compared to that in 100% Ca²⁺ medium, supporting the notion that the response of the DHA is also due, in part, to the opening of calcium channels. Furthermore, preincubation of cells with tyrphostin A9, an inhibitor of Ca²⁺ release–activated Ca²⁺ (CRAC) channels also significantly curtailed the DHA-induced sustained response on the increases in [Ca²⁺]i in these cells.

These results suggest that DHA induces an increase in [Ca²⁺]i via the ER pool and the opening of CRAC channels in human T-cells.—Bonin, A., and N. A. Kahn. Regulation of calcium signalling by docosahexaenoic acid in human T-cells: implication of CRAC channels. J. Lipid Res. 2000. 41: 277;–284.

Supplementary key words: polyunsaturated fatty acid, CD3, thapsigargin, Jurkat T-cells

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