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Journal of Lipid Research, Vol. 41, 357-367, March 2000
Copyright © 2000 by Lipid Research, Inc.
Studies of the metabolism of -tocopherol stereoisomers in rats using [5-methyl-14C]SRR- and RRR- -tocopherol
Kazuyo Kanekoa,
Chikako Kiyosea,
Tadahiko Uedab,
Hisatsugu Ichikawac, and
Osamu Igarashia
a Institute of Environmental Science for Human Life, Ochanomizu University, Bunkyo-ku, Tokyo 112-8610, Japan
b Tokyo Metropolitan Research Institute for Environmental Protection, Koto-ku, Tokyo 136-0075, Japan
c Tokyo Metropolitan Research Laboratory of Public Health, Shinjuku-ku, Tokyo 169-0073, Japan
Correspondence to:
Chikako Kiyose
We investigated the distribution and metabolism of SRR- -tocopherol (SRR- -Toc), synthetic -Toc compared with RRR- -Toc, in rats after a single oral administration of 2 mg (20 µCi) SRR- and RRR- -[5-methyl-14C]Toc. In the liver, there was no difference in the recovery of radioactivity until 12 h after administration, and it reached a maximum of 4.4% of the dose after 12 h, but in other tissues, radioactivity derived from RRR- -Toc was clearly higher than that derived from SRR- -Toc after 12 h. For 96 h after administration, urinary excretions of SRR- -Toc were 7.8% of the dose and significantly greater than that of RRR- -Toc, which was 1.3% of the dose. On the other hand, total fecal excretions of SRR- and RRR- -Toc were 87.6% and 83.0%, respectively. Therefore, radioactivity in the urine was assumed to have transferred out of the liver. Furthermore, the urine samples were hydrolyzed with 3 N methanolic HCl and analyzed by high performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry.
The results showed that about 73% of the total radioactivity injected into HPLC was found to be 2,5,7,8-tetramethyl-2-(2'-carboxyethyl)-6-hydroxy chroman ( -CEHC), as well as RRR- -Toc. Thus, there is no difference between SRR- -Toc and RRR- -Toc in metabolic pathways, and it is suggested that SRR- -Toc discriminated in the liver is rapidly metabolized by the liver and excreted as the conjugate of -CEHC in the urine.Kaneko, K., C. Kiyose, T. Ueda, H. Ichikawa, and O. Igarishi. Studies of the metabolism of -tocopherol stereoisomers in rats using [5-methy1-14C]SRR- and RRR- -tocopherol. J. Lipid Res. 2000. 41: 357;367.
Supplementary key words:
RRR- -tocopherol, SRR- -tocopherol, RRR- -[5-methyl-14C]tocopherol, SRR- -[5-methyl-14C]tocopherol, -CEHC, -tocopherol metabolism in vivo, distribution of radioactivity in rat, detection of LC-MS, urine metabolites

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Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.
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