HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kaneko, K. Articles by Igarashi, O. Search for Related Content PubMed PubMed Citation Articles by Kaneko, K. Articles by Igarashi, O. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 41, 357-367, March 2000
Copyright © 2000 by Lipid Research, Inc.

Original Article

Studies of the metabolism of -tocopherol stereoisomers in rats using [5-methyl-¹⁴C]SRR- and RRR--tocopherol

Correspondence to: Chikako Kiyose

We investigated the distribution and metabolism of SRR--tocopherol (SRR--Toc), synthetic -Toc compared with RRR--Toc, in rats after a single oral administration of 2 mg (20 µCi) SRR- and RRR--[5-methyl-¹⁴C]Toc. In the liver, there was no difference in the recovery of radioactivity until 12 h after administration, and it reached a maximum of 4.4% of the dose after 12 h, but in other tissues, radioactivity derived from RRR--Toc was clearly higher than that derived from SRR--Toc after 12 h. For 96 h after administration, urinary excretions of SRR--Toc were 7.8% of the dose and significantly greater than that of RRR--Toc, which was 1.3% of the dose. On the other hand, total fecal excretions of SRR- and RRR--Toc were 87.6% and 83.0%, respectively. Therefore, radioactivity in the urine was assumed to have transferred out of the liver. Furthermore, the urine samples were hydrolyzed with 3 N methanolic HCl and analyzed by high performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry.

The results showed that about 73% of the total radioactivity injected into HPLC was found to be 2,5,7,8-tetramethyl-2-(2'-carboxyethyl)-6-hydroxy chroman (-CEHC), as well as RRR--Toc. Thus, there is no difference between SRR--Toc and RRR--Toc in metabolic pathways, and it is suggested that SRR--Toc discriminated in the liver is rapidly metabolized by the liver and excreted as the conjugate of -CEHC in the urine.—Kaneko, K., C. Kiyose, T. Ueda, H. Ichikawa, and O. Igarishi. Studies of the metabolism of -tocopherol stereoisomers in rats using [5-methy1-¹⁴C]SRR- and RRR--tocopherol. J. Lipid Res. 2000. 41: 357;–367.

Supplementary key words: RRR--tocopherol, SRR--tocopherol, RRR--[5-methyl-¹⁴C]tocopherol, SRR--[5-methyl-¹⁴C]tocopherol, -CEHC, -tocopherol metabolism in vivo, distribution of radioactivity in rat, detection of LC-MS, urine metabolites

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				T. J. Sontag and R. S. Parker Influence of major structural features of tocopherols and tocotrienols on their {omega}-oxidation by tocopherol-{omega}-hydroxylase J. Lipid Res., May 1, 2007; 48(5): 1090 - 1098. [Abstract] [Full Text] [PDF]

				H. Saito, C. Kiyose, H. Yoshimura, T. Ueda, K. Kondo, and O. Igarashi {gamma}-Tocotrienol, a vitamin E homolog, is a natriuretic hormone precursor J. Lipid Res., August 1, 2003; 44(8): 1530 - 1535. [Abstract] [Full Text] [PDF]

				M. Schuelke, A. Elsner, B. Finckh, A. Kohlschutter, C. Hubner, and R. Brigelius-Flohe Urinary {alpha}-tocopherol metabolites in {alpha}-tocopherol transfer protein-deficient patients J. Lipid Res., October 1, 2000; 41(10): 1543 - 1551. [Abstract] [Full Text]