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Journal of Lipid Research, Vol. 41, 514-520, April 2000
Copyright © 2000 by Lipid Research, Inc.

Original Article

Peroxisome proliferator-activated receptor (PPAR) and agonist inhibit cholesterol 7-hydroxylase gene (CYP7A1) transcription

Correspondence to: John Y. L. Chiang

Fibrates are widely used hypolipidemic drugs that regulate the expression of many genes involved in lipid metabolism by activating the peroxisome proliferator-activated receptor (PPAR). The objective of this study was to investigate the mechanism of action of peroxisome proliferators and PPAR on the transcription of cholesterol 7-hydroxylase, the rate-limiting enzyme in the conversion of cholesterol to bile acids in the liver. When cotransfected with the expression vectors for PPAR and RXR, Wy14,643 reduced human and rat cholesterol 7-hydroxylase gene (CYP7A1)/luciferase reporter activities by 88% and 43%, respectively, in HepG2 cells, but not in CV-1 or CHO cells. We have mapped the peroxisome proliferator response element (PPRE) to a conserved sequence containing the canonical AGGTCA direct repeats separated by one nucleotide (DR1). This DR1 sequence was mapped previously as a binding site for the hepatocyte nuclear factor 4 (HNF-4) which stimulates CYP7A1 transcription. Electrophoretic mobility shift assay (EMSA) showed no direct binding of in vitro synthesized PPAR/RXR heterodimer to the DR1 sequence. PPAR and Wy14,643 did not affect HNF-4 binding to the DR1. However, Wy14,643 and PPAR/RXR significantly reduced HNF-4 expression in HepG2 cells.

These results suggest that PPAR and agonist repress cholesterol 7-hydroxylase activity by reducing the availability of HNF-4 for binding to the DR-1 sequence and therefore attenuates the transactivation of CYP7A1 by HNF- 4.—Marrapodi, M., and J. Y. L. Chiang. Peroxisome proliferator-activated receptor (PPAR) and agonist inhibit cholesterol 7-hydroxylase gene (CYP7A1) transcription. J. Lipid Res. 2000. 41: 514;–520.

Supplementary key words: bile acid synthesis, gallstones, peroxisome proliferators, hypolipidemic drugs, cytochrome P450, HNF-4

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