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Correspondence to:
Dennis E. Vance
The transcription factor Sp1 has been implicated in regulation of the expression of the murine CTP:phosphocholine cytidylyltransferase
The results suggest that the level of expression of the CT
Supplementary key words:
gene regulation, promoter response elements, Drosophila SL2 cells, C3H10T1/2 cells
Copyright © 2000 by Lipid Research, Inc.
Original Article
Functional significance of Sp1, Sp2, and Sp3 transcription factors in regulation of the murine CTP:phosphocholine cytidylyltransferase
Marica Bakovica,
Kristin A. Waitea, and
Dennis E. Vancea
promoter
a Department of Biochemistry and CIHR Group on Molecular and Cell Biology of Lipids, University of Alberta, Edmonton, Alberta, T6G 2S2, Canada
(CT
) gene, Ctpct (M. Bakovic, K. Waite, W. Tang, I. Tabas, and D. E. Vance. 1999. Biochim. Biophys. Acta. 1438: 147;165). We have utilized transient transfections, mutation analysis, electromobility gel-shifts, and immunoblot analysis to test the hypothesis that expression of the CT
gene is controlled in part by the binding of three trans-acting nuclear factors, Sp1, Sp2, and Sp3. Sp1 and Sp3 activate CT
gene transcription through sequence specific binding within three promoter domains. In Sp1-mediated transcription, Sp3 acts as an activator in a dose-dependent manner and vice versa. Sp2 represses Sp1- and Sp3-driven transcription in Drosophila SL2 cells, but stimulates transcription in C3H10T1/2 mammalian cells. Our results suggest that the predominant action of Sp proteins is a direct function of local organization of three cis-acting elements in the regions A (-31/-9), B (-88/-50), and C (-148/-128). The ability of distal C (-148/-128) and proximal A (-31/-9) regions to activate or repress transcription depends upon the cellular background. The multiple binding elements at position B (-88/-50) confer a positive regulation independent of the cell context. However, the effectiveness of Sp proteins at this site is strongly governed by neighboring sites A and C.
gene will depend on the cell type, the availability of Sp proteins, and the structure and organization of three cis-acting elements.Bakovic, M., K. A. Waite, and D. E. Vance. Functional significance of Spl, Sp2, and Sp3 transcription factors in regulation of the murine CTP:phosphocholine cytidylyltransferase
promoter. J. Lipid Res. 41: 583;594. ![]()
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