HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Agirre, A. Articles by Dijkstra, J. Search for Related Content PubMed PubMed Citation Articles by Agirre, A. Articles by Dijkstra, J. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 41, 621-628, April 2000
Copyright © 2000 by Lipid Research, Inc.

Original Article

Induction of aggregation and fusion of cholesterol-containing membrane vesicles by an anti-cholesterol monoclonal antibody

Correspondence to: José L. Nieva

A monoclonal IgM antibody that reacts with cholesterol was able to aggregate small and large unilamellar lipid vesicles. Vesicles aggregated by the antibody could be dispersed by trypsin digestion. Inclusion of unsaturated phosphatidylethanolamine in the vesicle formulation lowered the relative amount of cholesterol necessary for aggregation, and prevented disaggregation by trypsin treatment. Fluorimetric assays indicated that membrane mixing occurred in aggregates resistant to trypsinization, but the vesicles did not mix or leak their aqueous contents. Analysis of the kinetics of lipid-mixing showed an increase in the aggregation and fusion rate constants with increasing antibody concentrations, indicating that the antibody reaction promotes both processes. An apparent inactivation process whose rate increased with antibody dose has been considered.

We conclude that the simultaneous binding of antibodies to more than one vesicle at densities that allow the contact of membrane surfaces, induces first aggregation followed by hemifusion, and with excess of antibody also results in inactivation of the latter process.—Agirre, A., S. Nir, J. L. Nieva, and J. Dijkstra. Induction of aggregation and fusion of cholesterol-containing membrane vesicles by an anti-cholesterol monoclonal antibody. J. Lipid Res. 2000. 41: 621;–628.

Supplementary key words: anticholesterol monoclonal antibody, cholesterol, membrane fusion, lipid-mixing, membrane hemifusion, membrane aggregation, large unilamellar vesicle, small unilamellar vesicle, trypsin, IgM

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. Agirre, A. Barco, L. Carrasco, and J. L. Nieva Viroporin-mediated Membrane Permeabilization. PORE FORMATION BY NONSTRUCTURAL POLIOVIRUS 2B PROTEIN J. Biol. Chem., October 18, 2002; 277(43): 40434 - 40441. [Abstract] [Full Text] [PDF]