HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Murphy, E. J. Articles by Schroeder, F. Search for Related Content PubMed PubMed Citation Articles by Murphy, E. J. Articles by Schroeder, F. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 41, 788-796, May 2000
Copyright © 2000 by Lipid Research, Inc.

Original Article

Sterol carrier protein-2 expression alters phospholipid content and fatty acyl composition in L-cell fibroblasts

Correspondence to: Eric J. Murphy

The effects sterol carrier protein-2 (SCP-2) expression on L-cell phospholipid levels and fatty acyl composition was assessed using L-cells transfected with the murine cDNA encoding for either the 15 kDa proSCP-2 or 13.2 kDa SCP-2. Expression of these proteins reduced total phospholipid mass (nmol/mg protein) by 24% and reduced the cholesterol to phospholipid ratio 60 and 28%, respectively. In 15 kDa proSCP-2 expressing cells, individual phospholipid class masses, excluding sphingomyelin (CerPCho), were reduced as follows: phosphatidylinositol (PtdIns) and phosphatidylserine (PtdSer) >> ethanolamine glycerophospholipid (EtnGpl) > choline glycerophospholipid (ChoGpl). Furthermore, ethanolamine plasmalogen mass was decreased 25%, while choline plasmalogen mass was elevated 30% in 15 kDa proSCP-2 expressing cells. In 13.2 kDa SCP-2 expressing cells, phospholipid class mass was decreased as follows: PtdIns and PtdSer >> ChoGpl. These changes in phospholipid mass resulted in altered cellular phospholipid composition. Expression of either protein differentially altered the type of fatty acid esterified onto the phospholipids. These effects included a greater proportion of polyunsaturated fatty acids and a reduction in saturated fatty acids, although 15 kDa proSCP-2 expression had a more robust effect on these parameters than did 13.2 kDa SCP-2 expression. In summary, expression of SCP-2 reduced individual phospholipid class mass, except for CerPCho, and altered the fatty acid composition of each phospholipid class examined.

These results clearly demonstrate that SCP-2 expression altered basal phospholipid levels, suggesting that SCP-2 can alter the function of endoplasmic reticulum phospholipid synthetic enzymes.—Murphy, E. J., T. Stiles, and F. Schroeder. Sterol carrier protein-2 expression alters phospholipid content and fatty acyl composition in L-cell fibroblasts. J. Lipid Res. 2000. 41: 788;–796.

Supplementary key words: sterol carrier protein-2, phospholipids, plasmalogens, fatty acids, L-cells, fibroblasts

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. E. Ellis, E. J. Murphy, D. C. Mitchell, M. Y. Golovko, F. Scaglia, G. C. Barcelo-Coblijn, and R. L. Nussbaum Mitochondrial Lipid Abnormality and Electron Transport Chain Impairment in Mice Lacking {alpha}-Synuclein Mol. Cell. Biol., November 15, 2005; 25(22): 10190 - 10201. [Abstract] [Full Text] [PDF]

				L. Amigo, S. Zanlungo, J. F. Miquel, J. M. Glick, H. Hyogo, D. E. Cohen, A. Rigotti, and F. Nervi Hepatic overexpression of sterol carrier protein-2 inhibits VLDL production and reciprocally enhances biliary lipid secretion J. Lipid Res., February 1, 2003; 44(2): 399 - 407. [Abstract] [Full Text] [PDF]

				H. Huang, O. Starodub, A. McIntosh, A. B. Kier, and F. Schroeder Liver Fatty Acid-binding Protein Targets Fatty Acids to the Nucleus. REAL TIME CONFOCAL AND MULTIPHOTON FLUORESCENCE IMAGING IN LIVING CELLS J. Biol. Chem., August 2, 2002; 277(32): 29139 - 29151. [Abstract] [Full Text] [PDF]

				F. Schroeder, A. Frolov, O. Starodub, B. B. Atshaves, W. Russell, A. Petrescu, H. Huang, A. M. Gallegos, A. McIntosh, D. Tahotna, et al. Pro-sterol Carrier Protein-2. ROLE OF THE N-TERMINAL PRESEQUENCE IN STRUCTURE, FUNCTION, AND PEROXISOMAL TARGETING J. Biol. Chem., August 11, 2000; 275(33): 25547 - 25555. [Abstract] [Full Text] [PDF]

				B. P. Atshaves, S. M. Storey, A. L. McIntosh, A. D. Petrescu, O. I. Lyuksyutova, A. S. Greenberg, and F. Schroeder Sterol Carrier Protein-2 Expression Modulates Protein and Lipid Composition of Lipid Droplets J. Biol. Chem., June 29, 2001; 276(27): 25324 - 25335. [Abstract] [Full Text] [PDF]