|
Journal of Lipid Research, Vol. 41, 873-881, June 2000
Copyright © 2000 by Lipid Research, Inc.
Prostaglandin E2 regulation of cyclooxygenase expression in keratinocytes is mediated via cyclic nucleotide-linked prostaglandin receptors
Regina E. Maldvea,
Youngsoo Kima,
Stephanie J. Mugaa, and
Susan M. Fischera
a The University of Texas M.D. Anderson Cancer Center, Science Park-Research Division, P.O. Box 389, Smithville, TX 78957
Correspondence to:
Susan M. Fischer
Inflammatory responses are thought to be mediated in part by the prostaglandins derived from arachidonic acid (AA) by the action of prostaglandin H synthase, also referred to as cyclooxygenase (COX). The mitogen-inducible isoform, COX-2, is over-expressed in numerous chronic inflammatory disease conditions and in neoplasms from both human and experimental animal models. COX-1 expression, on the other hand, has been referred to as constitutive or non-inducible. In this study, we present evidence demonstrating autoregulation of prostaglandin (PG) production by the PGs themselves and their precursor, AA. We observed that AA and PGs induced COX-2, as well as COX-1, expression in cultured murine keratinocytes approximately 3 h after treatment. In primary keratinocytes transiently transfected with a full-length COX-2 promoter linked to a luciferase reporter gene, we observed enhanced transcription by AA, PGE2, and the other prostaglandins. Forskolin, a known activator of adenylate cyclase, and dibutryl-cAMP, a cAMP analog, induced COX-1 and COX-2 mRNA, suggesting that cAMP is a second messenger for COX expression. SQ 22536, an adenylate cyclase inhibitor, inhibited COX-2 mRNA induction by PGE2 in a dose-dependent manner suggesting that PGE2-induced expression may be through one of the cAMP-linked PGE2 receptors.
The results of this study demonstrate that both COX-1 and COX-2 are inducible. Further, both COX isoforms can be up-regulated by their products, the PGs, and this autoregulation probably occurs via prostaglandin receptors linked to a cAMP signal transduction pathway.Maldve, R. E., Y. Kim, S. J. Muga, and S. M. Fischer. Prostaglandin E2 regulation of cyclooxygenase expression in keratinocytes is mediated via cyclic nucleotide-linked prostaglandin receptors. J. Lipid Res. 2000. 41: 873;881.
Supplementary key words:
prostaglandins, cyclooxygenase, signal transduction

CiteULike Complore Connotea Del.icio.us Digg Reddit Technorati What's this?
This article has been cited by other articles:

|
 |

|
 |
 
K. M. Ansari, J. E. Rundhaug, and S. M. Fischer
Multiple Signaling Pathways Are Responsible for Prostaglandin E2-Induced Murine Keratinocyte Proliferation
Mol. Cancer Res.,
June 1, 2008;
6(6):
1003 - 1016.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
K. M. Ansari, Y. M. Sung, G. He, and S. M. Fischer
Prostaglandin receptor EP2 is responsible for cyclooxygenase-2 induction by prostaglandin E2 in mouse skin
Carcinogenesis,
October 1, 2007;
28(10):
2063 - 2068.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
H. N. Jabbour, R. W. Kelly, H. M. Fraser, and H. O. D. Critchley
Endocrine Regulation of Menstruation
Endocr. Rev.,
February 1, 2006;
27(1):
17 - 46.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y. M. Sung, G. He, and S. M. Fischer
Lack of Expression of the EP2 but not EP3 Receptor for Prostaglandin E2 Results in Suppression of Skin Tumor Development
Cancer Res.,
October 15, 2005;
65(20):
9304 - 9311.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
H. Jiang, A. S. Weyrich, G. A. Zimmerman, and T. M. McIntyre
Endothelial Cell Confluence Regulates Cyclooxygenase-2 and Prostaglandin E2 Production That Modulate Motility
J. Biol. Chem.,
December 31, 2004;
279(53):
55905 - 55913.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
P. Yang, D. Chan, E. Felix, C. Cartwright, D. G. Menter, T. Madden, R. D. Klein, S. M. Fischer, and R. A. Newman
Formation and antiproliferative effect of prostaglandin E3 from eicosapentaenoic acid in human lung cancer cells
J. Lipid Res.,
June 1, 2004;
45(6):
1030 - 1039.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
R. K. Petersen, C. Jorgensen, A. C. Rustan, L. Froyland, K. Muller-Decker, G. Furstenberger, R. K. Berge, K. Kristiansen, and L. Madsen
Arachidonic acid-dependent inhibition of adipocyte differentiation requires PKA activity and is associated with sustained expression of cyclooxygenases
J. Lipid Res.,
December 1, 2003;
44(12):
2320 - 2330.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
D. L. Gessell-Lee, V. L. Popov, I. Boldogh, J. P. Olano, and J. W. Peterson
Role of Cyclooxygenase Enzymes in a Murine Model of Experimental Cholera
Infect. Immun.,
November 1, 2003;
71(11):
6234 - 6242.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
L. Pang, M. Nie, L. Corbett, and A. J. Knox
Cyclooxygenase-2 Expression by Nonsteroidal Anti-inflammatory Drugs in Human Airway Smooth Muscle Cells: Role of Peroxisome Proliferator-Activated Receptors
J. Immunol.,
January 15, 2003;
170(2):
1043 - 1051.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
K. J. Sales, A. A. Katz, R. P. Millar, and H. N. Jabbour
Seminal plasma activates cyclooxygenase-2 and prostaglandin E2 receptor expression and signalling in cervical adenocarcinoma cells
Mol. Hum. Reprod.,
December 1, 2002;
8(12):
1065 - 1070.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M.-H. Wu, H.S. Sun, C.-C. Lin, K.-Y. Hsiao, P.-C. Chuang, H.-A. Pan, and S.-J. Tsai
Distinct mechanisms regulate cyclooxygenase-1 and -2 in peritoneal macrophages of women with and without endometriosis
Mol. Hum. Reprod.,
December 1, 2002;
8(12):
1103 - 1110.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y. Sun, X. M. Tang, E. Half, M. T. Kuo, and F. A. Sinicrope
Cyclooxygenase-2 Overexpression Reduces Apoptotic Susceptibility by Inhibiting the Cytochrome c-dependent Apoptotic Pathway in Human Colon Cancer Cells
Cancer Res.,
November 1, 2002;
62(21):
6323 - 6328.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
B. Hinz and K. Brune
Cyclooxygenase-2---10 Years Later
J. Pharmacol. Exp. Ther.,
February 1, 2002;
300(2):
367 - 375.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
K. J. Sales, A. A. Katz, B. Howard, R. P. Soeters, R. P. Millar, and H. N. Jabbour
Cyclooxygenase-1 Is Up-Regulated in Cervical Carcinomas: Autocrine/Paracrine Regulation of Cyclooxygenase-2, Prostaglandin E Receptors, and Angiogenic Factors by Cyclooxygenase-1
Cancer Res.,
January 1, 2002;
62(2):
424 - 432.
[Abstract]
[Full Text]
[PDF]
|
 |
|
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.
|
Advertisement
Advertisement
|