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Journal of Lipid Research, Vol. 41, 933-939, June 2000
Copyright © 2000 by Lipid Research, Inc.

Original Article

-Cyclodextrin extracts diacylglycerol from insect high density lipoproteins

Correspondence to: Zeina E. Jouni

-Cyclodextrins are water-soluble cyclic hexamers of glucose units with hydrophobic cavities capable of solubilizing lipophiles. Incubating -cyclodextrin with high density lipophorin from Manduca sexta or Bombyx mori resulted in a cloudy, turbid solution. Centrifugation separated a pale yellowish precipitate. Thin-layer chromatography analysis of the lipid extract of the precipitate showed that the major lipid was diacylglycerol, while KBr density gradient analysis of the supernatant demonstrated the presence of a lipid-depleted very high density lipophorin. Transfer of diacylglycerol from lipophorin to cyclodextrin was specific to -cyclodextrin and was not observed with ß- or -cyclodextrins. pH had no effect on diacylglycerol transfer to -cyclodextrin. However, the transfer was strongly dependent on the concentration of -cyclodextrin and temperature. Increasing the concentration of -cyclodextrin in the incubation mixture was associated with the formation of increasingly higher density lipophorins. Thus, at 20, 30, and 40 mM -cyclodextrin, the density of B. mori lipophorin increased from 1.107 g/ml to 1.123, 1.148, and 1.181 g/ml, respectively. At concentrations greater than 40 mM, -cyclodextrin had no further effect on the density of lipophorin. -Cyclodextrin removed at most 83;–87% of the diacylglycerol present in lipophorin. Temperature played an important role in altering the amount of diacylglycerols transferred to -cyclodextrin. At 30 mM -cyclodextrin, the amount of diacylglycerol transferred at different temperatures was 50% at 4°C, 41% at 15°C, 20% at 28°C, and less than 3% at 37°C.

We propose that diacylglycerol transfers to -cyclodextrin via an aqueous diffusion pathway and that the driving force for the transfer is the formation of an insoluble -cyclodextrin–diacylglycerol complex.—Jouni, Z. E., J. Zamora, M. Snyder, W. R. Montfort, A. Weichsel, and M. A. Wells. -Cyclodextrin extracts diacylglycerol from insect high density lipoproteins. J. Lipid Res. 2000. 41: 933;–939.

Supplementary key words: lipophorin, fluid-phase transfer, Manduca sexta, Bombyx mori

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