HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Waterworth, D. M. Articles by Talmud, P. J. Search for Related Content PubMed PubMed Citation Articles by Waterworth, D. M. Articles by Talmud, P. J. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 41, 1103-1109, July 2000
Copyright © 2000 by Lipid Research, Inc.

Original Article

Plasma levels of remnant particles are determined in part by variation in the APOC3 gene insulin response element and the APOCI;–APOE cluster

Correspondence to: D. M. Waterworth

Remnant particles of triglyceride-rich lipoproteins (RLP) are known to be a strong predictor of atherogenicity. The serum concentrations of remnant-like particle triglyceride (RLPTG) and remnant-like particle cholesterol (RLPC) have been determined in a representative sample of the Czech MONICA study (n = 285). The relationship was investigated between remnant particle triglyceride/cholesterol concentrations and polymorphisms in the genes APOC3 (-482CT/3238CG), APOE (2/3/4), APOCI (-317-321ins), APOB (signal peptide), hepatic lipase (LIPE, -480CT), and lipoprotein lipase (LPL, S447X). Univariate analysis showed significant effects on RLPTG associated only with the APOE genotype (P = 0.009), the APOC3 -482CT genotype (P = 0.018), and the APOCI -317-321ins (P = 0.014) genotype and significant effects on RLPC with APOE (P = 0.01) and APOCI -317-321ins (P = 0.021). The raising effect of the APOE genotype for both remnant cholesterol and triglyceride was confined to the 2/4 (n = 6) and 4/4 (n = 3) groups, and thus when the 2/4 group was omitted in order to analyze by allele (2+/3+/4+), significance was lost (P = 0.6). There was strong linkage disequilibrium between the APOE and APOCI alleles (², P < 0.001) and a multivariate ANOVA of RLPTG with all three significantly associated variants as factors demonstrated that while the APOC3 -482CT effect was independent of the others (P = 0.003), the APOCI -317-321ins and APOE effects were not. This was also true for the APOCI -317-321ins and APOE effects on RLPC. To assess whether APOE-CI effects on RLPC were independent of their effects on total cholesterol and triglyceride levels, multiple linear regression was used. Using multiple linear regression, it appeared that the APOE-CI effects on RLPC were independent of their effects on plasma cholesterol, but the effects of APOC3 and APOE-CI on RLPTG could not be separated from their effects on plasma Tg levels.

Further characterization of this remnant particle phenotype and its genetic determinants may lead to a better understanding of its metabolism and contribution to atherosclerosis.—Waterworth, D. M., J. A. Hubacek, J. Pitha, J. Kovar, R. Poledne, S. E. Humphries, and P. J. Talmud. Plasma levels of remnant particles are determined in part by variation in the APOC3 gene insulin response element and the APOCI;–APOE cluster. J. Lipid Res. 2000. 41: 1103;–1109.

Supplementary key words: insulin response element, remnant-like triglyceride, remnant-like cholesterol, hepatic lipase, lipoprotein lipase, apolipoprotein B

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. E. Hokanson, G. L. Kinney, S. Cheng, H. A. Erlich, A. Kretowski, and M. Rewers Susceptibility to Type 1 Diabetes Is Associated With ApoCIII Gene Haplotypes Diabetes, March 1, 2006; 55(3): 834 - 838. [Abstract] [Full Text] [PDF]

				O. Olivieri, C. Stranieri, A. Bassi, B. Zaia, D. Girelli, F. Pizzolo, E. Trabetti, S. Cheng, M. A. Grow, P. F. Pignatti, et al. ApoC-III gene polymorphisms and risk of coronary artery disease J. Lipid Res., September 1, 2002; 43(9): 1450 - 1457. [Abstract] [Full Text] [PDF]

				T. C. Ooi, M. Cousins, D. S. Ooi, G. Steiner, K. D. Uffelman, K. Nakajima, and I. E. Simo Postprandial Remnant-like Lipoproteins in Hypertriglyceridemia J. Clin. Endocrinol. Metab., July 1, 2001; 86(7): 3134 - 3142. [Abstract] [Full Text] [PDF]