J. Lipid Res.
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Journal of Lipid Research, Vol. 41, 1177-1180, July 2000
Copyright © 2000 by Lipid Research, Inc.

methods

Single polyprenol and dolichol isolation by semipreparative high-performance liquid chromatography technique

Tomas Carlsona,b, Karolina Skorupinska-Tudekc, Jozefina Hertelc, Tadeusz Chojnackic, Jerker M. Olssona, and Ewa Swiezewskac
a Department of Immunology, Microbiology, Pathology, and Infectious Diseases, Karolinska Institute, Division of Pathology, Huddinge University Hospital, SE-141 86 Huddinge, Sweden
b Sodertorns Hogskola, University College, SE-141 04 Huddinge, Sweden
c Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland

Correspondence to: Ewa Swiezewska

A new method of separation of single polyprenols (or dolichols) from a mixture of isoprenoid alcohols is described. Application of a high-performance liquid chromatography (HPLC) apparatus equipped with a semipreparative ODS column resulted in preparation of long-chain (dihydro)polyprenols of high purity (>95%).

This approach substantially decreases the time scale of the conventional chromatographical preparative procedure. The method can be widely used in chemical and biochemical projects, where single polyprenols or dolichols are required.—Carlson, T., K. Skorupinska-Tudek, J. Hertel, T. Chojnacki, J. M. Olsson, and E. Swiezewska. Single polyprenol and dolichol isolation by semipreparative high-performance liquid chromatography technique. J. Lipid Res. 2000. 41: 1177;–1180.

Supplementary key words: polyprenol, dolichol, preparative HPLC


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