HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Stamler, C. J. Articles by Sparks, D. L. Search for Related Content PubMed PubMed Citation Articles by Stamler, C. J. Articles by Sparks, D. L. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 41, 1214-1221, August 2000
Copyright © 2000 by Lipid Research, Inc.

Original Article

Phosphatidylinositol promotes cholesterol transport in vivo

Correspondence to: Daniel L. Sparks

To examine the role that lipoprotein charge plays in cholesterol metabolism in vivo, we characterized the effects of an intravenous injection of 40 µmol of an uncharged phospholipid (phosphatidylcholine, PC) or an anionic phospholipid (phosphatidylinositol, PI) into fasted rabbits. PC injection had a negligible effect on lipoprotein charge and composition, similar to that observed in a saline-injected animal. In contrast, PI injection caused a significant increase in the net negative surface charge of all lipoproteins after only 10 min, followed by a gradual return to normal by 24 h. Lipoprotein compositional analysis showed that PI caused a significant increase of cholesteryl ester (CE) and cholesterol (FC) in the VLDL pool by 3 h, with no changes in VLDL-triglyceride content. While the bulk of the plasma CE was located in the HDL pool in the PC-injected animals, in the PI animals, VLDL became the major CE storage compartment. No major changes in the levels or composition of HDL or LDL were evident over the 24-h turnover period. Co-injection of [³H]FC revealed a 30-fold greater rate of clearance of the labeled cholesterol from the PI-injected rabbit plasma. In addition, the rate of cholesterol esterification by lecithin:cholesterol acyltransferase was almost completely inhibited in the PI animals.

In summary, a bolus injection of PI into rabbits appears to enhance the mobilization of cellular sterol and promote a rapid clearance of both FC and CE from the plasma compartment. The data show that lipoprotein charge can affect cholesterol transport and that this process can be selectively manipulated.—Stamler, C. J., D. Breznan, T. A-M. Neville, F. J. Viau, E. Camlioglu, and D. L. Sparks. Phosphatidylinositol promotes cholesterol transport in vivo. J. Lipid Res. 2000. 41: 1214–1221.

Supplementary key words: caveolae, lipid clearance, cholesteryl ester, transfer protein, lipoprotein charge, scavenger receptor

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. W. Burgess, T. A-M. Neville, P. Rouillard, Z. Harder, D. S. Beanlands, and D. L. Sparks Phosphatidylinositol increases HDL-C levels in humans J. Lipid Res., February 1, 2005; 46(2): 350 - 355. [Abstract] [Full Text] [PDF]

				K. M. Wasan, O. Sivak, R. A. Cote, A. I. MacInnes, K. D. Boulanger, M. Lynn, W. J. Christ, L. D. Hawkins, and D. P. Rossignol Association of the Endotoxin Antagonist E5564 with High-Density Lipoproteins In Vitro: Dependence on Low-Density and Triglyceride-Rich Lipoprotein Concentrations Antimicrob. Agents Chemother., September 1, 2003; 47(9): 2796 - 2803. [Abstract] [Full Text] [PDF]

				J. W. Burgess, J. Boucher, T. A-M. Neville, P. Rouillard, C. Stamler, S. Zachariah, and D. L. Sparks Phosphatidylinositol promotes cholesterol transport and excretion J. Lipid Res., July 1, 2003; 44(7): 1355 - 1363. [Abstract] [Full Text] [PDF]