HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tadano-Aritomi, K. Articles by Ishizuka, I. Search for Related Content PubMed PubMed Citation Articles by Tadano-Aritomi, K. Articles by Ishizuka, I. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 41, 1237-1243, August 2000
Copyright © 2000 by Lipid Research, Inc.

Original Article

Kidney lipids in galactosylceramide synthase-deficient mice: absence of galactosylsulfatide and compensatory increase in more polar sulfoglycolipids

Correspondence to: Ineo Ishizuka

UDP-galactose:ceramide galactosyltransferase (CGT) catalyzes the final step in the synthesis of galactosylceramide (GalCer). It has previously been shown that CGT-deficient mice do not synthesize GalCer and its sulfated derivative GalCer I³-sulfate (galactosylsulfatide, SM4s) but form myelin containing glucosylceramide (GlcCer) and sphingomyelin with 2-hydroxy fatty acids. Because relatively high concentrations of GalCer and SM4s are present also in mammalian kidney, we analyzed the composition of lipids in the kidney of Cgt^-/- and, as a control, Cgt^+/- and wild-type mice. The homozygous mutant mice lacked GalCer, galabiaosylceramide (Ga₂Cer), and SM4s. Yet, they did not show any major morphological or functional defects in the kidney. A slight increase in GlcCer containing 4-hydroxysphinganine was evident among neutral glycolipids. Intriguingly, more polar sulfoglycolipids, that is, lactosylceramide II³-sulfate (SM3) and gangliotetraosylceramide II³,IV³-bis-sulfate (SB1a), were expressed at 2 to 3 times the normal levels in Cgt^-/- mice, indicating upregulation of biosynthesis of SB1a from GlcCer via SM3. Given that SM4s is a major polar glycolipid constituting renal tubular membrane, the increase in SM3 and SB1a in the mice deficient in CGT and thus SM4s appears to be a compensatory process, which could partly restore kidney function in the knockout mice.—Tadano-Aritomi, K., T. Hikita, H. Fujimoto, K. Suzuki, K. Motegi, and I. Ishizuka. Kidney lipids in galactosylceramide synthase-deficient mice: absence of galactosylsulfatide and compensatory increase in more polar sulfoglycolipids. J. Lipid Res. 2000. 41: 1237;–1243.

Supplementary key words: UDP-galactose:ceramide galactosyltransferase, gene targeting, glycolipids, galactosylceramide, sulfatides, sulfolipids, sulfoglycolipids, kidney, liquid-SIMS, TLC blotting

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Z. V. Popovic, R. Sandhoff, T. P. Sijmonsma, S. Kaden, R. Jennemann, E. Kiss, E. Tone, F. Autschbach, N. Platt, E. Malle, et al. Sulfated Glycosphingolipid as Mediator of Phagocytosis: SM4s Enhances Apoptotic Cell Clearance and Modulates Macrophage Activity J. Immunol., November 15, 2007; 179(10): 6770 - 6782. [Abstract] [Full Text] [PDF]

				J. Matsuda, M. Kido, K. Tadano-Aritomi, I. Ishizuka, K. Tominaga, K. Toida, E. Takeda, K. Suzuki, and Y. Kuroda Mutation in saposin D domain of sphingolipid activator protein gene causes urinary system defects and cerebellar Purkinje cell degeneration with accumulation of hydroxy fatty acid-containing ceramide in mouse Hum. Mol. Genet., November 1, 2004; 13(21): 2709 - 2723. [Abstract] [Full Text] [PDF]

				D. Ogawa, K. Shikata, K. Honke, S. Sato, M. Matsuda, R. Nagase, A. Tone, S. Okada, H. Usui, J. Wada, et al. Cerebroside Sulfotransferase Deficiency Ameliorates L-selectin-dependent Monocyte Infiltration in the Kidney after Ureteral Obstruction J. Biol. Chem., January 16, 2004; 279(3): 2085 - 2090. [Abstract] [Full Text] [PDF]

				K. Tadano-Aritomi, J. Matsuda, H. Fujimoto, K. Suzuki, and I. Ishizuka Seminolipid and its precursor/degradative product, galactosylalkylacylglycerol, in the testis of saposin A- and prosaposin-deficient mice J. Lipid Res., September 1, 2003; 44(9): 1737 - 1743. [Abstract] [Full Text] [PDF]

				R. Sandhoff, S. T. Hepbildikler, R. Jennemann, R. Geyer, V. Gieselmann, R. L. Proia, H. Wiegandt, and H.-J. Grone Kidney Sulfatides in Mouse Models of Inherited Glycosphingolipid Disorders. DETERMINATION BY NANO-ELECTROSPRAY IONIZATION TANDEM MASS SPECTROMETRY J. Biol. Chem., May 31, 2002; 277(23): 20386 - 20398. [Abstract] [Full Text] [PDF]

				K. Tadano-Aritomi, T. Hikita, A. Suzuki, H. Toyoda, T. Toida, T. Imanari, and I. Ishizuka Determination of lipid-bound sulfate by ion chromatography and its application to quantification of sulfolipids from kidneys of various mammalian species J. Lipid Res., October 1, 2001; 42(10): 1604 - 1608. [Abstract] [Full Text] [PDF]

				K. Ogura and T. Tai Characterization of the functional domains of galactosylceramide expression factor 1 in MDCK cells Glycobiology, September 1, 2001; 11(9): 751 - 758. [Abstract] [Full Text] [PDF]