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Journal of Lipid Research, Vol. 41, 1244-1251, August 2000
Copyright © 2000 by Lipid Research, Inc.
Expression of nitric oxide synthases in subcutaneous adipose tissue of nonobese and obese humans
Montserrat Elizaldea,
Mikael Rydéna,
Vanessa van Harmelena,
Peter Enerothe,
Hans Gyllenhammarb,
Cecilia Holmf,
Stig Ramelc,
Anders Ölundd,
Peter Arnera, and
Kurt Anderssona
a Center for Metabolism and Endocrinology, Huddinge Hospital, 141 86 Huddinge, Sweden
b Departments of Hematology, Huddinge Hospital, 141 86 Huddinge, Sweden
c Surgery, Huddinge Hospital, 141 86 Huddinge, Sweden
d Gynecology, Huddinge Hospital, 141 86 Huddinge, Sweden
e Unit for Applied Biochemistry, Huddinge Hospital, 141 86 Huddinge, Sweden
f Department of Cell and Molecular Biology, Lund University, 22185 Lund, Sweden
Correspondence to:
Kurt Andersson
Studies have shown evidence of production of nitric oxide (NO) in adipose tissue, as well as inhibition of lipolysis by NO. We have analyzed nitric oxide synthase (NOS) expression in subcutaneous adipose tissue from 13 nonobese and 18 obese male subjects. Using a competitive reverse transcription polymerase chain reaction method, endothelial (eNOS) and inducible (iNOS), but not neuronal (nNOS), nitric oxide synthase mRNA expression was detected in isolated fat cells and pieces of adipose tissue. Tissue mRNA levels for eNOS were 3,814 ± 825 and 5,956 ± 476 amol/mg RNA (P = 0.043), and for iNOS 306 ± 38 and 332 ± 48 amol/mg RNA, for nonobese and obese individuals, respectively. Western blotting revealed similar eNOS protein levels in isolated fat cells and adipose tissue pieces. Protein levels for eNOS in nonobese and obese individuals, respectively, were (in optical density [OD] units per mm2 per 100 µg of total protein) 0.11 ± 0.08 and 2.80 ± 1.30 (P = 0.043). iNOS protein was detectable, but not measurable, at low levels in a subset of obese patients (3 of 10). iNOS protein levels could not be detected in nonobese individuals. Hormone-sensitive lipase (HSL), the key regulating enzyme in lipolysis, is reduced in obesity. The expression of HSL protein in subcutaneous adipose tissue was studied in the same subset of patients; in agreement with previous results, HSL levels were reduced in obese subjects: 4.64 ± 1.10 and 1.27 ± 0.35 (P = 0.012) in nonobese and obese subjects, respectively.
In conclusion, this study shows that eNOS and iNOS, but not nNOS, are present in human subcutaneous adipose tissue. Gene expression and protein levels of eNOS are increased, whereas HSL protein levels are decreased in obesity. It is speculated that increased NO production, preferably by eNOS, and decreased HSL levels may cause decreased subcutaneous adipose tissue lipolysis in obesity.Elizalde, M., M. Rydén, V. van Harmelen, P. Eneroth, H. Gyllenhammar, C. Holm, S. Ramel, A. Ölund, P. Arner, and K. Andersson. Expression of nitric oxide synthases in subcutaneous adipose tissue of nonobese and obese humans. J. Lipid Res. 2000. 41: 1244;1251.
Supplementary key words:
fat cells, NOS, hormone-sensitive lipase, messenger RNA, protein

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Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.
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