| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Original Article |
Correspondence to: James M. Ntambi
Two stearoyl-CoA desaturase (SCD) isoforms can be expressed during the differentiation of 3T3-L1 preadipocytes into adipocytes. Here we report on the effects of the peroxisome proliferator-activated receptor 
ligand troglitazone (TRO) on scd1 and scd2 mRNA levels as determined by Northern blotting, on SCD protein expression as determined by Western blotting, and on total lipid composition as determined by GC during differentiation. In preadipocytes, scd1 mRNA and SCD protein were not detected, whereas scd2 mRNA was detected. These cells have high levels of palmitate (16:0), stearate (18:0), and monounsaturated oleate (
9-18:1) and low levels of monounsaturated palmitoleate (9-16:1). In MDI (methylisobutylxanthine, dexamethasone, and insulin)-treated cells, scd1 mRNA and SCD protein were increased 
100-fold relative to preadipocyte levels, the scd2 mRNA level was increased 2-fold, 9-16:1 was increased 20-fold, and 18:0 was decreased 3-fold. In TRO-treated cells, the scd1 mRNA level was lower than that observed in preadipocytes, while the scd2 mRNA level was similar. TRO also decreased scd1 mRNA in primary adipocytes. The TRO-treated cells contained a 9-18:1 level typical of MDI-treated cells whereas, conversely, these cells also contained a low 9-16:1 level typical of preadipocytes. The implications of these correlations for the regulatory and enzymatic mechanism(s) used to establish and maintain lipid composition are discussed.—Kim, Y-C., F. E. Gomez, B. G. Fox, and J. M. Ntambi. Differential regulation of the stearoyl-CoA desaturase genes by thiazolidinediones in 3T3-L1 adipocytes. J. Lipid Res. 2000. 41: 1310;–1316.
Supplementary key words:
3T3-L1 adipocyte, stearoyl-CoA desaturase, adipocyte lipid-binding protein, PPAR, gene expression, troglitazone, insulin
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  R. Mar-Heyming, M. Miyazaki, D. Weissglas-Volkov, N. A. Kolaitis, N. Sadaat, C. Plaisier, P. Pajukanta, R. M. Cantor, T. W.A. de Bruin, J. M. Ntambi, et al. Association of Stearoyl-CoA Desaturase 1 Activity With Familial Combined Hyperlipidemia Arterioscler. Thromb. Vasc. Biol., June 1, 2008; 28(6): 1193 - 1199. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Qin, J. Tian, P. Zhang, Y. Fan, L. Chen, Y. Guan, Y. Fu, Y. Zhu, S. Chien, and N. Wang Laminar shear stress up-regulates the expression of stearoyl-CoA desaturase-1 in vascular endothelial cells Cardiovasc Res, June 1, 2007; 74(3): 506 - 514. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Y. Cho, E. S. Shin, P. J. Park, D. W. Shin, H. K. Chang, D. Kim, H. H. Lee, J. H. Lee, S. H. Kim, M. J. Song, et al. Identification of Mouse Prp19p as a Lipid Droplet-associated Protein and Its Possible Involvement in the Biogenesis of Lipid Droplets J. Biol. Chem., January 26, 2007; 282(4): 2456 - 2465. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Rahman, A. Dobrzyn, S.-H. Lee, P. Dobrzyn, M. Miyazaki, and J. M. Ntambi Stearoyl-CoA desaturase 1 deficiency increases insulin signaling and glycogen accumulation in brown adipose tissue Am J Physiol Endocrinol Metab, February 1, 2005; 288(2): E381 - E387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Wang, B. Kurdi-Haidar, and J. F. Oram LXR-mediated activation of macrophage stearoyl-CoA desaturase generates unsaturated fatty acids that destabilize ABCA1 J. Lipid Res., May 1, 2004; 45(5): 972 - 980. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Castro-Chavez, V. K. Yechoor, P. K. Saha, J. Martinez-Botas, E. C. Wooten, S. Sharma, P. O'Connell, H. Taegtmeyer, and L. Chan Coordinated Upregulation of Oxidative Pathways and Downregulation of Lipid Biosynthesis Underlie Obesity Resistance in Perilipin Knockout Mice: A Microarray Gene Expression Profile Diabetes, November 1, 2003; 52(11): 2666 - 2674. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Brown, M. S. Boysen, S. S. Jensen, R. F. Morrison, J. Storkson, R. Lea-Currie, M. Pariza, S. Mandrup, and M. K. McIntosh Isomer-specific regulation of metabolism and PPAR{gamma} signaling by CLA in human preadipocytes J. Lipid Res., July 1, 2003; 44(7): 1287 - 1300. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Miyazaki, F. E. Gomez, and J. M. Ntambi Lack of stearoyl-CoA desaturase-1 function induces a palmitoyl-CoA {Delta}6 desaturase and represses the stearoyl-CoA desaturase-3 gene in the preputial glands of the mouse J. Lipid Res., December 1, 2002; 43(12): 2146 - 2154. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Gerhold, F. Liu, G. Jiang, Z. Li, J. Xu, M. Lu, J. R. Sachs, A. Bagchi, A. Fridman, D. J. Holder, et al. Gene Expression Profile of Adipocyte Differentiation and Its Regulation by Peroxisome Proliferator-Activated Receptor-{gamma} Agonists Endocrinology, June 1, 2002; 143(6): 2106 - 2118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tabernero, A. Velasco, B. Granda, E. M. Lavado, and J. M. Medina Transcytosis of Albumin in Astrocytes Activates the Sterol Regulatory Element-binding Protein-1, Which Promotes the Synthesis of the Neurotrophic Factor Oleic Acid J. Biol. Chem., February 1, 2002; 277(6): 4240 - 4246. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Miyazaki, H.-J. Kim, W. C. Man, and J. M. Ntambi Oleoyl-CoA Is the Major de Novo Product of Stearoyl-CoA Desaturase 1 Gene Isoform and Substrate for the Biosynthesis of the Harderian Gland 1-Alkyl-2,3-diacylglycerol J. Biol. Chem., October 12, 2001; 276(42): 39455 - 39461. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Miyazaki, W. C. Man, and J. M. Ntambi Targeted Disruption of Stearoyl-CoA Desaturase1 Gene in Mice Causes Atrophy of Sebaceous and Meibomian Glands and Depletion of Wax Esters in the Eyelid J. Nutr., September 1, 2001; 131(9): 2260 - 2268. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
