Advertisement
J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dobiásová, M.
Right arrow Articles by Frohlich, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dobiásová, M.
Right arrow Articles by Frohlich, J.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Journal of Lipid Research, Vol. 41, 1356-1357, August 2000
Copyright © 2000 by Lipid Research, Inc.


Original Article

Effect of labeling of plasma lipoproteins with [3H]cholesterol on values of esterification rate of cholesterol in apolipoprotein B-depleted plasma

Milada Dobiásováa, Lida Adlerb, Takao Ohtac, and Jiri Frohlichb
a Institute of Physiology, Academy of Sciences of the Czech Republic, Prague 14220, Czech Republic
b Department of Pathology and Laboratory Medicine, University of British Columbia, Healthy Heart Program/Lipid Clinic, St. Paul's Hospital, Vancouver, BC, Canada V6Z 1Y6
c Department of Pediatrics, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa 903-0215, Japan

Correspondence to: Jiri Frohlich

The fractional esterification rate of cholesterol in apolipoprotein B (apoB)-depleted plasma (FERHDL) is a good indicator of particle size distribution in high density lipoprotein (HDL) and low density lipoprotein (LDL). However, there has been a discrepancy in the absolute values of FERHDL published by different laboratories. Because the main difference between the methods was in the labeling of lipoproteins with [3H]cholesterol we investigated the effect of using Corning immunoplates and paper discs as carriers of the labeled unesterified cholesterol. We found that Corning plates trap some 3H-labeled free cholesterol, which is released during incubation at 37°C. This means that this additional 3H-labeled free cholesterol is exposed to lecithin: cholesterol acyltransferase (LCAT) for a shorter time and artificially decreases FERHDL. Using paper discs discarded before incubation as carriers of the 3H-labeled free cholesterol results in complete labeling of HDL and thus yields higher values of FERHDL.—Dobiásová, M., L. Adler, T. Ohta, and J. Frohlich. Effect of labeling of plasma lipoproteins with [3H]cholesterol on values of esterification rate of cholesterol in apolipoprotein B-depleted plasma. J. Lipid Res. 2000. 41: 1356;–1357.

Supplementary key words: [3H]cholesterol labeling of lipoproteins, LCAT, cholesterol esterification rate in apoB-depleted plasma, FERHDL


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
J. Lipid Res.Home page
M. Wroblewska, B. Kortas-Stempak, A. Szutowicz, and T. Badzio
Phospholipids mediated conversion of HDLs generates specific apoA-II pre-{beta} mobility particles
J. Lipid Res., April 1, 2009; 50(4): 667 - 675.
[Abstract] [Full Text] [PDF]


Home page
Clin. Chem.Home page
R. Movva and D. J. Rader
Laboratory Assessment of HDL Heterogeneity and Function
Clin. Chem., May 1, 2008; 54(5): 788 - 800.
[Abstract] [Full Text] [PDF]


Home page
Arterioscler. Thromb. Vasc. Bio.Home page
B. Zhang, P. Fan, E. Shimoji, H. Xu, K. Takeuchi, C. Bian, and K. Saku
Inhibition of Cholesteryl Ester Transfer Protein Activity by JTT-705 Increases Apolipoprotein E-Containing High-Density Lipoprotein and Favorably Affects the Function and Enzyme Composition of High-Density Lipoprotein in Rabbits
Arterioscler. Thromb. Vasc. Biol., October 1, 2004; 24(10): 1910 - 1915.
[Abstract] [Full Text] [PDF]


Home page
Clin. Chem.Home page
J. Frohlich and M. Dobiasova
Fractional Esterification Rate of Cholesterol and Ratio of Triglycerides to HDL-Cholesterol Are Powerful Predictors of Positive Findings on Coronary Angiography
Clin. Chem., November 1, 2003; 49(11): 1873 - 1880.
[Abstract] [Full Text] [PDF]


Home page
J. Lipid Res.Home page
N. R. Matthan, K. Cianflone, A. H. Lichtenstein, L. M. Ausman, M. Jauhiainen, and P. J. H. Jones
Hydrogenated fat consumption affects acylation- stimulating protein levels and cholesterol esterification rates in moderately hypercholesterolemic women
J. Lipid Res., November 1, 2001; 42(11): 1841 - 1848.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement