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Journal of Lipid Research, Vol. 41, 1356-1357, August 2000
Copyright © 2000 by Lipid Research, Inc.

Original Article

Effect of labeling of plasma lipoproteins with [³H]cholesterol on values of esterification rate of cholesterol in apolipoprotein B-depleted plasma

Milada Dobiáová

Correspondence to: Jiri Frohlich

The fractional esterification rate of cholesterol in apolipoprotein B (apoB)-depleted plasma (FER_HDL) is a good indicator of particle size distribution in high density lipoprotein (HDL) and low density lipoprotein (LDL). However, there has been a discrepancy in the absolute values of FER_HDL published by different laboratories. Because the main difference between the methods was in the labeling of lipoproteins with [³H]cholesterol we investigated the effect of using Corning immunoplates and paper discs as carriers of the labeled unesterified cholesterol. We found that Corning plates trap some ³H-labeled free cholesterol, which is released during incubation at 37°C. This means that this additional ³H-labeled free cholesterol is exposed to lecithin: cholesterol acyltransferase (LCAT) for a shorter time and artificially decreases FER_HDL. Using paper discs discarded before incubation as carriers of the ³H-labeled free cholesterol results in complete labeling of HDL and thus yields higher values of FER_HDL.—Dobiáová, M., L. Adler, T. Ohta, and J. Frohlich. Effect of labeling of plasma lipoproteins with [³H]cholesterol on values of esterification rate of cholesterol in apolipoprotein B-depleted plasma. J. Lipid Res. 2000. 41: 1356;–1357.

Supplementary key words: [³H]cholesterol labeling of lipoproteins, LCAT, cholesterol esterification rate in apoB-depleted plasma, FER_HDL

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