HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lawrence, J. W. Articles by Eacho, P. I. Search for Related Content PubMed PubMed Citation Articles by Lawrence, J. W. Articles by Eacho, P. I. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 41, 1390-1401, September 2000
Copyright © 2000 by Lipid Research, Inc.

Original Article

Ligand-dependent interaction of hepatic fatty acid-binding protein with the nucleus

Correspondence to: Jeffrey W. Lawrence

Our studies were conducted to explore the role of hepatic fatty acid-binding protein (L-FABP) in fatty acid transport to the nucleus. Purified rat L-FABP facilitated the specific interaction of [³H]oleic acid with the nuclei. L-FABP complexed with unlabeled oleic acid decreased the nuclear association of [³H]oleic acid:L-FABP; however, oleic acid-saturated bovine serum albumin (BSA) or fatty acid-free L-FABP did not. The peroxisome-proliferating agents LY171883, bezafibrate, and WY-14,643 were also effective competitors when complexed to L-FABP. Nuclease treatment did not affect the nuclear association of [³H]oleic acid:L-FABP; however, proteinase treatment of the nuclei abolished the binding. Nuclei incubated with fluorescein-conjugated L-FABP in the presence of oleic acid were highly fluorescent whereas no fluorescence was observed in reactions lacking oleic acid, suggesting that L-FABP itself was binding to the nuclei. The nuclear binding of FABP was concentration dependent, saturable, and competitive. LY189585, a ligand for L-FABP, also facilitated the nuclear binding of fluorescein-conjugated L-FABP, although it was less potent than oleic acid. A structural analog that does not bind L-FABP, LY163443, was relatively inactive in stimulating the nuclear binding. Potential interactions between L-FABP and nuclear proteins were analyzed by Far-Western blotting and identified a 33-kDa protein in the 500 mM NaCl extract of rat hepatocyte nuclei that bound strongly to biotinylated L-FABP. Oleic acid enhanced the interaction of L-FABP with the 33-kDa protein as well as other nuclear proteins.

We propose that L-FABP is involved in communicating the state of fatty acid metabolism from the cytosol to the nucleus through an interaction with lipid mediators that are involved in nuclear signal transduction.—Lawrence, J. W., D. J. Kroll, and P. I. Eacho. Ligand-dependent interaction of hepatic fatty acid-binding protein with the nucleus. J. Lipid Res. 2000. 41: 1390;–1401.

Supplementary key words: fatty acid-binding protein, LY171883, fatty acids, bezafibrate, oleic acid, WY-14,643, peroxisome proliferators, nuclear transport

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				H. A. Hostetler, A. L. McIntosh, B. P. Atshaves, S. M. Storey, H. R. Payne, A. B. Kier, and F. Schroeder L-FABP directly interacts with PPAR{alpha} in cultured primary hepatocytes J. Lipid Res., August 1, 2009; 50(8): 1663 - 1675. [Abstract] [Full Text] [PDF]

				G. G. Martin, B. P. Atshaves, A. L. McIntosh, J. T. Mackie, A. B. Kier, and F. Schroeder Liver Fatty Acid-Binding Protein Gene-Ablated Female Mice Exhibit Increased Age-Dependent Obesity J. Nutr., October 1, 2008; 138(10): 1859 - 1865. [Abstract] [Full Text] [PDF]

				C. Le May, M. Cauzac, C. Diradourian, D. Perdereau, J. Girard, A.-F. Burnol, and J.-P. Pegorier Fatty Acids Induce L-CPT I Gene Expression through a PPAR{alpha}-Independent Mechanism in Rat Hepatoma Cells J. Nutr., October 1, 2005; 135(10): 2313 - 2319. [Abstract] [Full Text] [PDF]

				K. Suzuki, T. Babazono, H. Murata, and Y. Iwamoto Clinical Significance of Urinary Liver-Type Fatty Acid-Binding Protein in Patients With Diabetic Nephropathy Diabetes Care, August 1, 2005; 28(8): 2038 - 2039. [Full Text] [PDF]

				T. G. Brock, Y.-J. Lee, E. Maydanski, T. L. Marburger, M. Luo, R. Paine III, and M. Peters-Golden Nuclear localization of leukotriene A4 hydrolase in type II alveolar epithelial cells in normal and fibrotic lung Am J Physiol Lung Cell Mol Physiol, August 1, 2005; 289(2): L224 - L232. [Abstract] [Full Text] [PDF]

				B. P. Atshaves, A. L. McIntosh, H. R. Payne, J. Mackie, A. B. Kier, and F. Schroeder Effect of branched-chain fatty acid on lipid dynamics in mice lacking liver fatty acid binding protein gene Am J Physiol Cell Physiol, March 1, 2005; 288(3): C543 - C558. [Abstract] [Full Text] [PDF]

				A. Kamijo, T. Sugaya, A. Hikawa, M. Okada, F. Okumura, M. Yamanouchi, A. Honda, M. Okabe, T. Fujino, Y. Hirata, et al. Urinary Excretion of Fatty Acid-Binding Protein Reflects Stress Overload on the Proximal Tubules Am. J. Pathol., October 1, 2004; 165(4): 1243 - 1255. [Abstract] [Full Text] [PDF]

				E. P. Newberry, Y. Xie, S. Kennedy, X. Han, K. K. Buhman, J. Luo, R. W. Gross, and N. O. Davidson Decreased Hepatic Triglyceride Accumulation and Altered Fatty Acid Uptake in Mice with Deletion of the Liver Fatty Acid-binding Protein Gene J. Biol. Chem., December 19, 2003; 278(51): 51664 - 51672. [Abstract] [Full Text] [PDF]

				A. D. Petrescu, H. R. Payne, A. Boedecker, H. Chao, R. Hertz, J. Bar-Tana, F. Schroeder, and A. B. Kier Physical and Functional Interaction of Acyl-CoA-binding Protein with Hepatocyte Nuclear Factor-4{alpha} J. Biol. Chem., December 19, 2003; 278(51): 51813 - 51824. [Abstract] [Full Text] [PDF]

				M. Luo, S. M. Jones, M. Peters-Golden, and T. G. Brock Nuclear localization of 5-lipoxygenase as a determinant of leukotriene B4 synthetic capacity PNAS, October 14, 2003; 100(21): 12165 - 12170. [Abstract] [Full Text] [PDF]

				A. Pawar and D. B. Jump Unsaturated Fatty Acid Regulation of Peroxisome Proliferator-activated Receptor {alpha} Activity in Rat Primary Hepatoctes J. Biol. Chem., September 19, 2003; 278(38): 35931 - 35939. [Abstract] [Full Text] [PDF]

				H. Huang, O. Starodub, A. McIntosh, A. B. Kier, and F. Schroeder Liver Fatty Acid-binding Protein Targets Fatty Acids to the Nucleus. REAL TIME CONFOCAL AND MULTIPHOTON FLUORESCENCE IMAGING IN LIVING CELLS J. Biol. Chem., August 2, 2002; 277(32): 29139 - 29151. [Abstract] [Full Text] [PDF]

				N.-S. Tan, N. S. Shaw, N. Vinckenbosch, P. Liu, R. Yasmin, B. Desvergne, W. Wahli, and N. Noy Selective Cooperation between Fatty Acid Binding Proteins and Peroxisome Proliferator-Activated Receptors in Regulating Transcription Mol. Cell. Biol., July 15, 2002; 22(14): 5114 - 5127. [Abstract] [Full Text] [PDF]

				D. Linden, K. Lindberg, J. Oscarsson, C. Claesson, L. Asp, L. Li, M. Gustafsson, J. Boren, and S.-O. Olofsson Influence of Peroxisome Proliferator-activated Receptor alpha Agonists on the Intracellular Turnover and Secretion of Apolipoprotein (Apo) B-100 and ApoB-48 J. Biol. Chem., June 14, 2002; 277(25): 23044 - 23053. [Abstract] [Full Text] [PDF]

				A. W. Norris and A. A. Spector Very long chain n-3 and n-6 polyunsaturated fatty acids bind strongly to liver fatty acid-binding protein J. Lipid Res., April 1, 2002; 43(4): 646 - 653. [Abstract] [Full Text] [PDF]

				L. Carlsson, D. Linden, M. Jalouli, and J. Oscarsson Effects of fatty acids and growth hormone on liver fatty acid binding protein and PPAR{alpha} in rat liver Am J Physiol Endocrinol Metab, October 1, 2001; 281(4): E772 - E781. [Abstract] [Full Text] [PDF]

				C. Wolfrum, C. M. Borrmann, T. Borchers, and F. Spener Fatty acids and hypolipidemic drugs regulate peroxisome proliferator-activated receptors alpha - and gamma -mediated gene expression via liver fatty acid binding protein: A signaling path to the nucleus PNAS, February 15, 2001; (2001) 51619898. [Abstract] [Full Text]

				C. Wolfrum, C. M. Borrmann, T. Borchers, and F. Spener Fatty acids and hypolipidemic drugs regulate peroxisome proliferator-activated receptors alpha - and gamma -mediated gene expression via liver fatty acid binding protein: A signaling path to the nucleus PNAS, February 27, 2001; 98(5): 2323 - 2328. [Abstract] [Full Text] [PDF]