HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Penumetcha, M. Articles by Parthasarathy, S. Search for Related Content PubMed PubMed Citation Articles by Penumetcha, M. Articles by Parthasarathy, S. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 41, 1473-1480, September 2000
Copyright © 2000 by Lipid Research, Inc.

Original Article

Dietary oxidized fatty acids: an atherogenic risk?

Correspondence to: Sampath Parthasarathy

Previous studies have suggested that heated fat that contains oxidized fatty acids in the diet might contribute to the presence of oxidized components in circulating lipoproteins. On the other hand, studies in our laboratory showed that cultured cells such as smooth muscle cells take up oxidized fatty acids poorly. Because intestinal cells are morphologically quite distinct, we studied the uptake of oxidized linoleic acid by Caco-2 and smooth muscle cells (control). When 16-day-old Caco-2 cells were incubated with oxidized linoleic acid (ox-linoleic acid), its uptake was comparable to that of unoxidized linoleic acid (unox-linoleic acid) or that of oleic acid (40;–58, 70, and 55%, respectively). In contrast, the uptake of ox-linoleate by smooth muscle cells was about 3%. To determine whether the brush border structure of Caco-2 cells was responsible for increased uptake of oxidized fatty acids, we compared uptake in 4- and 16-day-old cells. The uptake of unox-linoleate and oleic acid (18:1) was comparable for the 4- and 16-day cells. In addition, saturation and competition experiments showed that the uptake of ox-linoleate by Caco-2 cells is not saturable even at 150 µM and that this uptake is diluted in the presence of unox-linoleate. In esterification experiments utilizing rat intestinal microsomes, we show that both ox- and unox-linoleate are esterified equally well.

In summary, dietary oxidized fatty acids can be absorbed by the intestine and incorporated into lipoproteins and could potentially impose an oxidative stress and exacerbate atherogenesis.—Penumetcha, M., N. Khan, and S. Parthasarathy. Dietary oxidized fatty acids: an atherogenic risk? J. Lipid Res. 2000. 41: 1473;–1480.

Supplementary key words: brush border, smooth muscle cells, lipid absorption, chylomicron, oxidized low-density lipoprotein, atherosclerosis

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. Eder, U. Keller, F. Hirche, and C. Brandsch Thermally Oxidized Dietary Fats Increase the Susceptibility of Rat LDL to Lipid Peroxidation but Not Their Uptake by Macrophages J. Nutr., September 1, 2003; 133(9): 2830 - 2837. [Abstract] [Full Text] [PDF]

				N. Khan-Merchant, M. Penumetcha, O. Meilhac, and S. Parthasarathy Oxidized Fatty Acids Promote Atherosclerosis Only in the Presence of Dietary Cholesterol in Low-Density Lipoprotein Receptor Knockout Mice J. Nutr., November 1, 2002; 132(11): 3256 - 3262. [Abstract] [Full Text] [PDF]

				M. Penumetcha, N. Khan-Merchant, and S. Parthasarathy Enhanced solubilization and intestinal absorption of cholesterol by oxidized linoleic acid J. Lipid Res., June 1, 2002; 43(6): 895 - 903. [Abstract] [Full Text] [PDF]

				R. Rong, S. Ramachandran, M. Penumetcha, N. Khan, and S. Parthasarathy Dietary oxidized fatty acids may enhance intestinal apolipoprotein A-I production J. Lipid Res., April 1, 2002; 43(4): 557 - 564. [Abstract] [Full Text] [PDF]