HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bodennec, J. Articles by Portoukalian, J. Search for Related Content PubMed PubMed Citation Articles by Bodennec, J. Articles by Portoukalian, J. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 41, 1524-1531, September 2000
Copyright © 2000 by Lipid Research, Inc.

Methods

A procedure for fractionation of sphingolipid classes by solid-phase extraction on aminopropyl cartridges

Correspondence to: J. Bodennec

Solid-phase extraction (SPE) methods are easy, rapid, and reliable. Their growing popularity is in part due to their operational simplicity and cost reduction in solvents, and partly because they are easier to automate. Sphingolipids are implicated in various cellular events such as growth, differentiation, and apoptosis. However, their separation by small SPE cartridges has attracted limited attention. Here we describe an SPE procedure on aminopropyl cartridges that by sequential elution allows the separation of a lipid mixture into free ceramides, neutral glycosphingolipids, neutral phospholipids (sphingomyelin), and a fraction containing the acidic phospholipids and phosphorylated sphingoid bases, phosphoceramides and sulfatides. Individual components are obtained in high yield and purity. We applied the procedure to obtain data on separation of [³H]myristic acid-labeled sphingolipids from fish gills, and from human melanoma tumor tissue. Individual lipids in the SPE fractions were identified by chromatography on several high-performance thin-layer chromatography (HPTLC) systems. The chromatographic behavior of free sphingoid bases is also reported.—Bodennec, J., O. Koul, I. Aguado, G. Brichon, G. Zwingelstein, and J. Portoukalian. A procedure for fractionation of sphingolipid classes by solid-phase extraction on aminopropyl cartridges. J. Lipid Res. 2000. 41: 1524;–1531.

Supplementary key words: ceramides, sphingosine, dihydrosphingosine, phytosphingosine, neutral glycosphingolipids, sphingomyelin, ceramide 1-phosphate, sphingosine 1-phosphate, sulfatides, solid-phase extraction

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				N. Vaisman, N. Kaysar, Y. Zaruk-Adasha, D. Pelled, G. Brichon, G. Zwingelstein, and J. Bodennec Correlation between changes in blood fatty acid composition and visual sustained attention performance in children with inattention: effect of dietary n-3 fatty acids containing phospholipids Am. J. Clinical Nutrition, May 1, 2008; 87(5): 1170 - 1180. [Abstract] [Full Text] [PDF]

				N. Gallardo, E. Bonzon-Kulichenko, T. Fernandez-Agullo, E. Molto, S. Gomez-Alonso, P. Blanco, J. M. Carrascosa, M. Ros, and A. Andres Tissue-Specific Effects of Central Leptin on the Expression of Genes Involved in Lipid Metabolism in Liver and White Adipose Tissue Endocrinology, December 1, 2007; 148(12): 5604 - 5610. [Abstract] [Full Text] [PDF]

				B. Toth, A. Balla, H. Ma, Z. A. Knight, K. M. Shokat, and T. Balla Phosphatidylinositol 4-Kinase IIIbeta Regulates the Transport of Ceramide between the Endoplasmic Reticulum and Golgi J. Biol. Chem., November 24, 2006; 281(47): 36369 - 36377. [Abstract] [Full Text] [PDF]

				F. Debierre-Grockiego, L. Schofield, N. Azzouz, J. Schmidt, C. Santos de Macedo, M. A. J. Ferguson, and R. T. Schwarz Fatty Acids from Plasmodium falciparum Down-Regulate the Toxic Activity of Malaria Glycosylphosphatidylinositols. Infect. Immun., October 1, 2006; 74(10): 5487 - 5496. [Abstract] [Full Text] [PDF]

				T. Cohen, W. Auerbach, L. Ravid, J. Bodennec, A. Fein, A. H. Futerman, A. L. Joyner, and M. Horowitz The Exon 8-Containing Prosaposin Gene Splice Variant Is Dispensable for Mouse Development, Lysosomal Function, and Secretion Mol. Cell. Biol., March 15, 2005; 25(6): 2431 - 2440. [Abstract] [Full Text] [PDF]

				E. Norris-Cervetto, R. Callaghan, F. M. Platt, R. A. Dwek, and T. D. Butters Inhibition of Glucosylceramide Synthase Does Not Reverse Drug Resistance in Cancer Cells J. Biol. Chem., September 24, 2004; 279(39): 40412 - 40418. [Abstract] [Full Text] [PDF]

				B. Colsch, C. Afonso, I. Popa, J. Portoukalian, F. Fournier, J.-C. Tabet, and N. Baumann Characterization of the ceramide moieties of sphingoglycolipids from mouse brain by ESI-MS/MS: identification of ceramides containing sphingadienine J. Lipid Res., February 1, 2004; 45(2): 281 - 286. [Abstract] [Full Text] [PDF]

				I. Carrie, J. Portoukalian, R. Vicaretti, J. Rochford, S. Potvin, and G. Ferland Menaquinone-4 Concentration Is Correlated with Sphingolipid Concentrations in Rat Brain J. Nutr., January 1, 2004; 134(1): 167 - 172. [Abstract] [Full Text] [PDF]

				C. Riebeling, J. C. Allegood, E. Wang, A. H. Merrill Jr., and A. H. Futerman Two Mammalian Longevity Assurance Gene (LAG1) Family Members, trh1 and trh4, Regulate Dihydroceramide Synthesis Using Different Fatty Acyl-CoA Donors J. Biol. Chem., October 31, 2003; 278(44): 43452 - 43459. [Abstract] [Full Text] [PDF]

				J. Bodennec, S. Trajkovic-Bodennec, and A. H. Futerman Simultaneous quantification of lyso-neutral glycosphingolipids and neutral glycosphingolipids by N-acetylation with [3H]acetic anhydride J. Lipid Res., July 1, 2003; 44(7): 1413 - 1419. [Abstract] [Full Text] [PDF]

				J. Bodennec, D. Pelled, and A. H. Futerman Aminopropyl solid phase extraction and 2 D TLC of neutral glycosphingolipids and neutral lysoglycosphingolipids J. Lipid Res., January 1, 2003; 44(1): 218 - 226. [Abstract] [Full Text] [PDF]

				K. Venkataraman, C. Riebeling, J. Bodennec, H. Riezman, J. C. Allegood, M. C. Sullards, A. H. Merrill Jr., and A. H. Futerman Upstream of Growth and Differentiation Factor 1 (uog1), a Mammalian Homolog of the Yeast Longevity Assurance Gene 1 (LAG1), Regulates N-Stearoyl-sphinganine (C18-(Dihydro)ceramide) Synthesis in a Fumonisin B1-independent Manner in Mammalian Cells J. Biol. Chem., September 13, 2002; 277(38): 35642 - 35649. [Abstract] [Full Text] [PDF]