HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Field, F. J. Articles by Mathur, S. N. Search for Related Content PubMed PubMed Citation Articles by Field, F. J. Articles by Mathur, S. N. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 42, 1-8, January 2001
Copyright © 2001 by Lipid Research, Inc.

Original Article

Gene expression of sterol regulatory element-binding proteins in hamster small intestine

Correspondence to: F. Jeffrey Field, To whom correspondence should be addressed.

Gene expression of sterol regulatory element-binding proteins 1a, 1c, and 2 (SREBP-1a, -1c, and -2) and of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase, HMG-CoA reductase, and the low density lipoprotein (LDL) receptor was examined in hamster small intestine. SREBP-1c transcript predominated over SREBP-1a. mRNA levels for SREBP-1a, -1c, and -2, LDL receptor, and HMG-CoA synthase were highest in jejunum and ileum. Expression of SREBP-1a and SREBP-1c was highest in cells of the upper villus and decreased in cells of the lower villus. Gene expression of SREBP-2 was lowest in cells of the upper villus and increased in cells of the lower villus. Ileal SREBP-2 gene expression was highest in cells of the midvillus. mRNA levels for HMG-CoA synthase and the LDL receptor followed a pattern similar to that of SREBP-2. A positive correlation existed between SREBP-2 gene expression and rates of cholesterol synthesis. Fatty acid synthesis was highest in jejunum and ileum, correlating positively with the expression of SREBP-1c. Sterol influx into intestinal cells decreased mRNA levels of SREBP-2, HMG-CoA reductase, HMG-CoA synthase, and LDL receptor. In ileum, sterol influx decreased gene expression of SREBP-1a and increased expression of SREBP-1c.

The results suggest that SREBP-2 regulates cholesterol synthesis in the small intestine. SREBP-1a is a minor transcript and its expression does not correlate with cholesterol-synthesizing activity. SREBP-1c is a major transcript in small intestine and its expression along the length of the gut correlates with fatty acid synthesis. Sterols regulate gene expression of sterol-responsive genes, including SREBP-2, in small intestine. — Field, F. J., E. Born, S. Murthy, and S. N. Mathur. Gene expression of sterol regulatory element-binding proteins in hamster small intestine. J. Lipid Res. 2001. 42: 1;–8.

Supplementary key words: cholesterol synthesis, fatty acid synthesis, HMG-CoA reductase, HMG-CoA synthase, LDL receptor

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Z. Ravid, M. Bendayan, E. Delvin, A. T. Sane, M. Elchebly, J. Lafond, M. Lambert, G. Mailhot, and E. Levy Modulation of intestinal cholesterol absorption by high glucose levels: impact on cholesterol transporters, regulatory enzymes, and transcription factors Am J Physiol Gastrointest Liver Physiol, November 1, 2008; 295(5): G873 - G885. [Abstract] [Full Text] [PDF]

				C Thomas, J-F Landrier, D Gaillard, J Grober, M-C Monnot, A Athias, and P Besnard Cholesterol dependent downregulation of mouse and human apical sodium dependent bile acid transporter (ASBT) gene expression: molecular mechanism and physiological consequences Gut, September 1, 2006; 55(9): 1321 - 1331. [Abstract] [Full Text] [PDF]

				A. Uc and B. E. Britigan Does Heme Oxygenase-1 Have a Role in Caco-2 Cell Cycle Progression? Experimental Biology and Medicine, May 1, 2003; 228(5): 590 - 595. [Abstract] [Full Text] [PDF]

				S. Murthy, E. Born, S. N. Mathur, and F. J. Field LXR/RXR activation enhances basolateral efflux of cholesterol in CaCo-2 cells J. Lipid Res., July 1, 2002; 43(7): 1054 - 1064. [Abstract] [Full Text] [PDF]

				F. J. Field, E. Born, S. Murthy, and S. N. Mathur Regulation of sterol regulatory element-binding proteins by cholesterol flux in CaCo-2 cells J. Lipid Res., October 1, 2001; 42(10): 1687 - 1698. [Abstract] [Full Text] [PDF]

				F. J. Field, E. Born, S. Murthy, and S. N. Mathur Regulation of Sterol Regulatory Element-binding Proteins in Hamster Intestine by Changes in Cholesterol Flux J. Biol. Chem., May 11, 2001; 276(20): 17576 - 17583. [Abstract] [Full Text] [PDF]

				I. Zaghini, J.-F. Landrier, J. Grober, S. Krief, S. A. Jones, M.-C. Monnot, I. Lefrere, M. A. Watson, J. L. Collins, H. Fujii, et al. Sterol Regulatory Element-binding Protein-1c Is Responsible for Cholesterol Regulation of Ileal Bile Acid-binding Protein Gene in Vivo. POSSIBLE INVOLVEMENT OF LIVER-X-RECEPTOR J. Biol. Chem., January 4, 2002; 277(2): 1324 - 1331. [Abstract] [Full Text] [PDF]