J. Lipid Res.  Neurobiology of Lipids (ISSN1683-5506)
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Field, F. J.
Right arrow Articles by Mathur, S. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Field, F. J.
Right arrow Articles by Mathur, S. N.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Journal of Lipid Research, Vol. 42, 1-8, January 2001
Copyright © 2001 by Lipid Research, Inc.


Original Article

Gene expression of sterol regulatory element-binding proteins in hamster small intestine

F. Jeffrey Fielda, Ella Borna, Shubha Murthya, and Satya N. Mathura
a Department of Internal Medicine and Veterans Administration, University of Iowa, Iowa City, IA 52242

Correspondence to: F. Jeffrey Field, To whom correspondence should be addressed.

Gene expression of sterol regulatory element-binding proteins 1a, 1c, and 2 (SREBP-1a, -1c, and -2) and of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase, HMG-CoA reductase, and the low density lipoprotein (LDL) receptor was examined in hamster small intestine. SREBP-1c transcript predominated over SREBP-1a. mRNA levels for SREBP-1a, -1c, and -2, LDL receptor, and HMG-CoA synthase were highest in jejunum and ileum. Expression of SREBP-1a and SREBP-1c was highest in cells of the upper villus and decreased in cells of the lower villus. Gene expression of SREBP-2 was lowest in cells of the upper villus and increased in cells of the lower villus. Ileal SREBP-2 gene expression was highest in cells of the midvillus. mRNA levels for HMG-CoA synthase and the LDL receptor followed a pattern similar to that of SREBP-2. A positive correlation existed between SREBP-2 gene expression and rates of cholesterol synthesis. Fatty acid synthesis was highest in jejunum and ileum, correlating positively with the expression of SREBP-1c. Sterol influx into intestinal cells decreased mRNA levels of SREBP-2, HMG-CoA reductase, HMG-CoA synthase, and LDL receptor. In ileum, sterol influx decreased gene expression of SREBP-1a and increased expression of SREBP-1c.

The results suggest that SREBP-2 regulates cholesterol synthesis in the small intestine. SREBP-1a is a minor transcript and its expression does not correlate with cholesterol-synthesizing activity. SREBP-1c is a major transcript in small intestine and its expression along the length of the gut correlates with fatty acid synthesis. Sterols regulate gene expression of sterol-responsive genes, including SREBP-2, in small intestine. Field, F. J., E. Born, S. Murthy, and S. N. Mathur. Gene expression of sterol regulatory element-binding proteins in hamster small intestine. J. Lipid Res. 2001. 42: 1;–8.

Supplementary key words: cholesterol synthesis, fatty acid synthesis, HMG-CoA reductase, HMG-CoA synthase, LDL receptor


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
GutHome page
C Thomas, J-F Landrier, D Gaillard, J Grober, M-C Monnot, A Athias, and P Besnard
Cholesterol dependent downregulation of mouse and human apical sodium dependent bile acid transporter (ASBT) gene expression: molecular mechanism and physiological consequences
Gut, September 1, 2006; 55(9): 1321 - 1331.
[Abstract] [Full Text] [PDF]


Home page
Exp. Biol. Med.Home page
A. Uc and B. E. Britigan
Does Heme Oxygenase-1 Have a Role in Caco-2 Cell Cycle Progression?
Experimental Biology and Medicine, May 1, 2003; 228(5): 590 - 595.
[Abstract] [Full Text] [PDF]


Home page
J. Lipid Res.Home page
S. Murthy, E. Born, S. N. Mathur, and F. J. Field
LXR/RXR activation enhances basolateral efflux of cholesterol in CaCo-2 cells
J. Lipid Res., July 1, 2002; 43(7): 1054 - 1064.
[Abstract] [Full Text] [PDF]


Home page
J. Lipid Res.Home page
F. J. Field, E. Born, S. Murthy, and S. N. Mathur
Regulation of sterol regulatory element-binding proteins by cholesterol flux in CaCo-2 cells
J. Lipid Res., October 1, 2001; 42(10): 1687 - 1698.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
F. J. Field, E. Born, S. Murthy, and S. N. Mathur
Regulation of Sterol Regulatory Element-binding Proteins in Hamster Intestine by Changes in Cholesterol Flux
J. Biol. Chem., May 11, 2001; 276(20): 17576 - 17583.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
I. Zaghini, J.-F. Landrier, J. Grober, S. Krief, S. A. Jones, M.-C. Monnot, I. Lefrere, M. A. Watson, J. L. Collins, H. Fujii, et al.
Sterol Regulatory Element-binding Protein-1c Is Responsible for Cholesterol Regulation of Ileal Bile Acid-binding Protein Gene in Vivo. POSSIBLE INVOLVEMENT OF LIVER-X-RECEPTOR
J. Biol. Chem., January 4, 2002; 277(2): 1324 - 1331.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.