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Journal of Lipid Research, Vol. 42, 79-87, January 2001
Copyright © 2001 by Lipid Research, Inc.

Original Article

Effect of acylglyceride content on the structure and function of reconstituted high density lipoprotein particles

Correspondence to: Daniel L. Sparks, To whom correspondence should be addressed.

The effects of different acylglycerides on the conformation and charge of apolipoprotein A-I (apoA-I) have been investigated in reconstituted high density lipoproteins (LpA-I). Various amounts of diacylglycerol (DG) and triacylglycerol (TG) were incorporated into sonicated spherical LpA-I particles containing 2 molecules of apoA-I and 80 molecules of phospholipid. Inclusion of 30 molecules of TG into the LpA-I particle increases the net negative charge of apoA-I (-8.5 to -9.3 mV), but has little effect on the amount and thermodynamic stability of the helices in apoA-I. Incorporation of 30 molecules of DG into the lipoprotein complex promotes a small increase in the -helix content and stability, but greatly increases the net negative charge of apoA-I (-8.5 to -11.2 mV). Inclusion of DG increases the immunoreactivity of two epitopes in the N terminus of apoA-I, but decreases the exposure of a domain closer to the C terminus (residues 148;–186) of the apoprotein. In contrast, TG increases the exposure of epitopes over the entire apoA-I molecule; TG increases the immunoreactivity of epitopes for 13 different monoclonal antibodies to apoA-I. Incubations with purified lecithin:cholesterol acyltransferase show that cholesterol esterification is stimulated by DG, but inhibited by TG.

The data show that TG and DG have different effects on apoA-I structure and function and this suggests that the TG-to-DG ratio in HDL may directly affect the metabolism of this lipoprotein class. — Braschi, S., C. R. Coffill, T. A-M. Neville, D. M. Hutt, and D. L. Sparks. Effect of acylglyceride content on the structure and function of reconstituted high density lipoprotein particles. J. Lipid Res. 2001. 42: 79;–87.

Supplementary key words: HDL, apolipoprotein A-I, diglyceride, triglyceride, charge, stability, conformation, immunochemistry, LCAT

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				J. Boucher, T. A. Ramsamy, S. Braschi, D. Sahoo, T. A-M. Neville, and D. L. Sparks Apolipoprotein A-II regulates HDL stability and affects hepatic lipase association and activity J. Lipid Res., May 1, 2004; 45(5): 849 - 858. [Abstract] [Full Text] [PDF]