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Journal of Lipid Research, Vol. 42, 1671-1677, October 2001
Copyright © 2001 by Lipid Research, Inc.

Original Article

Anti-leptin receptor antibody mimics the stimulation of lipolysis induced by leptin in isolated mouse fat pads

Correspondence to: Hiroshi Ueki, To whom correspondence should be addressed., ueki{at}fupharm.fukuyama-u.ac.jp (E-mail)

An anti-leptin receptor polyclonal antibody (receptor antibody), as well as leptin, stimulated the release of free fatty acids from isolated mouse fat pads in a time-dependent manner. Following a 90-min incubation, maximal lipolysis was observed at 6 µg/ml receptor antibody and 0.1 nM leptin. The receptor antibody did not show any additive effect to the stimulation of lipolysis induced by leptin, suggesting that they exert their actions through a similar mechanism involving the leptin receptor. N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H-89), quin 2-AM, N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), and neomycin sulfate (neomycin) all potently inhibited the stimulation of lipolysis by the receptor antibody and leptin. Short-term incubation of the fat pads with the receptor antibody or leptin showed a transient increase in the cellular content of cAMP and myo-inositol 1,4,5-trisphosphate (IP₃) in similar concentrations to the free fatty acid release. Quin 2-AM and W-7 also inhibited the increase in cAMP content, suggesting that a Ca²⁺/calmodulin-dependent process may be involved in a part of the mechanism in which the receptor antibody and leptin exert their effects.

The increase in cellular IP₃ content via phosphoinositide-specific phospholipase C (PLC) sensitive to neomycin appears to be a primary step to initiate intracellular events. Both the receptor antibody and leptin may stimulate the lipolysis through mechanisms involving a transient increase in the cellular IP₃ content followed by cAMP production, which leads to the activation of cAMP-dependent protein kinase. — Kawaji, N., A. Yoshida, T. Motoyashiki, T. Morita, and H. Ueki. Anti-leptin receptor antibody mimics the stimulation of lipolysis induced by leptin in isolated mouse fat pads. J. Lipid Res. 2001. 42: 1671–1677.

Supplementary key words: Ca²⁺, myo-inositol 1,4,5-trisphosphate, cAMP

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