J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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Journal of Lipid Research, Vol. 42, 1841-1848, November 2001
Copyright © 2001 by Lipid Research, Inc.

Hydrogenated fat consumption affects acylation- stimulating protein levels and cholesterol esterification rates in moderately hypercholesterolemic women

Nirupa R. Matthana,b, Katherine Cianfloneb,c, Alice H. Lichtensteina, Lynne M. Ausmana, Matti Jauhiainend, and Peter J. H. Jonesb
a Cardiovascular Nutrition Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA
b School of Dietetics and Human Nutrition, McGill University-Macdonald Campus, Quebec, Canada
c Mike Rosenbloom Laboratory for Cardiovascular Disease, McGill University-Royal Victoria Hospital, Quebec, Canada
d Department of Biochemistry, National Public Health Institute, Helsinki, Finland

Correspondence to: Nirupa R. Matthan, at the Cardiovascular Nutrition Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, 711 Washington Street, Boston MA 02111., nmatthan{at}hnrc.tufts.edu (E-mail)

To determine whether hydrogenated fat consumption alters triglyceride metabolism and cholesterol esterification rates, 14 women (65–71 years of age) were provided with each of four diets for 5-week periods according to a randomized cross-over design. The experimental diets contained either soybean oil (SO), low trans squeeze (SQM), medium trans tub (TM), or high trans stick (SM) margarines. Triglyceride uptake by adipose tissue was determined by measuring plasma acylation-stimulating protein (ASP), FFA, glucose, and insulin levels, while rates of transfer and esterification rate of newly synthesized cholesterol (ER) were derived by using plasma CETP levels and the deuterium incorporation methodology. Plasma ASP levels were lowest (P < 0.05) in subjects on the SM diet (33.4 ± 12.7 nM) compared with the SO (48.7 ± 17.0 nM) and SQM (50.7 ± 15.7 nM) diets. Conversely, FFA were highest (P < 0.05) on the SM diet (0.86 ± 0.45 mM) relative to all the other diets. No differences were observed in plasma glucose and insulin levels among diets. A trend toward higher CETP levels after consumption of the SM diet was observed. However, the ER was lowest (P < 0.05) after the SM (0.111 ± 0.062 g·day-1) diet and highest after consumption of the SQM (0.216 ± 0.123 g·day-1) diet. In addition, ASP levels were negatively correlated with FFA (r = -0.63, P < 0.05), LDL cholesterol (r = -0.56, P < 0.05), and TG (r = -0.41, P < 0.05), whereas FFA was positively correlated with apolipoprotein B-containing lipoproteins (r = 0.58 and 0.47, for VLDL and LDL cholesterol, P < 0.05), and negatively correlated with HDL cholesterol (r = -0.51, P < 0.05). The ER was found to positively correlate with HDL cholesterol and HDL2 subfraction (r = 0.53 and 0.45, respectively, P < 0.05).

Taken together, these data demonstrate that the alterations in circulating lipid levels, commonly observed with consumption of hydrogenated fat-rich diets, can be explained in part by changes in ASP activity as well as newly synthesized cholesterol ER. — Matthan, N. R., K. Cianflone, A. H. Lichtenstein, L. M. Ausman, M. Jauhiainen, and P. J. H. Jones. Hydrogenated fat consumption affects acylation-stimulating protein levels and cholesterol esterification rates in moderately hypercholesterolemic women. J. Lipid Res. 2001. 42: 1841–1848.

Supplementary key words: CETP, dietary fat, HDL cholesterol, hydrogenation, LDL cholesterol, trans fatty acids, triglyceride metabolism


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