J. Lipid Res.
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Journal of Lipid Research, Vol. 42, 1865-1878, November 2001
Copyright © 2001 by Lipid Research, Inc.

Postnatal development of hepatocellular apolipoprotein B assembly and secretion in the rat

Dietmar Plonnéa, Hans-Peter Schulzea, Ulla Kahlerta, Kerstin Meltkea, Holger Seidolta, Andrew J. Bennettb, Ian J. Cartwrightc, Joan A. Higginsc, Uwe Tilla, and Rolf Dargela
a Institute of Pathobiochemistry, Department of Medicine, Friedrich-Schiller University, 07740 Jena, Germany
b School of Biosciences, Queens Medical Centre, University of Nottingham Medical School, Nottingham NG7 2UH, UK
c Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK

Correspondence to: Dietmar Plonné, To whom correspondence should be addressed., dplo{at}mti-n.uni-jena.de (E-mail)

In this study, we explored the paradox that in suckling rats the serum concentration of LDL is high although the liver secretes only minimal quantities of VLDL, the presumed precursor of LDL. Freshly isolated hepatocytes and hepatocytes in primary culture obtained from adult (90 days old) and suckling (17 days old) rats were used to investigate the synthesis and secretion of apolipoprotein B (apoB) and lipids as well as the density profile of secreted apoB-containing lipoproteins. Furthermore, the effects of dexamethasone and oleate on apoB biogenesis were investigated in primary cultures of hepatocytes from adult and suckling rats. Hepatocytes from suckling rats were unable to assemble mature VLDL but secreted apoB as primordial lipoprotein particles in the LDL-HDL density range. Intracellular degradation of apoB was also reduced in hepatocytes from suckling rats compared with that in hepatocytes from adults. The immaturity in VLDL assembly and apoB degradation of hepatocytes from suckling rats could be overcome by treating the cultures with dexamethasone plus oleate or dexamethasone alone. The lower microsomal triacylglycerol transfer protein (MTP) mRNA concentrations in hepatocytes from suckling rats in comparison with hepatocytes from adult rats were not reflected in lower MTP activity levels. Furthermore, dexamethasone plus oleate treatment had no effect on MTP activity although VLDL assembly and secretion were clearly stimulated.

We conclude that, during the suckling period of the rat, serum LDL is directly produced by the liver. This is a result of impaired hepatic VLDL assembly, which is a consequence of low triglyceride synthesis and an inefficient mobilization of bulk lipids in the second step of VLDL assembly. — Plonné, D., H-P. Schulze, U. Kahlert, K. Meltke, H. Seidolt, A. J. Bennett, I. J. Cartwright, J. A. Higgins, U. Till, and R. Dargel. Postnatal development of hepatocellular apolipoprotein B assembly and secretion in the rat. J. Lipid Res. 2001. 42: 1865–1878.

Supplementary key words: atherosclerosis, direct LDL secretion, primary hepatocytes, rat development


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