HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sellers, J. A. Articles by Shelness, G. S. Search for Related Content PubMed PubMed Citation Articles by Sellers, J. A. Articles by Shelness, G. S. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 42, 1897-1904, November 2001
Copyright © 2001 by Lipid Research, Inc.

Lipoprotein assembly capacity of the mammary tumor-derived cell line C127 is due to the expression of functional microsomal triglyceride transfer protein

Correspondence to: Gregory S. Shelness, To whom correspondence should be addressed., gshelnes{at}wfubmc.edu (E-mail)

C127, a murine mammary tumor-derived cell line, is capable of lipidating and secreting apolipoprotein B-41 (apoB-41) in the apparent absence of microsomal triglyceride transfer protein (MTP). Using a semiquantitative reverse transcriptase-coupled polymerase chain reaction, mouse MTP mRNA was detected in C127 cells at 10–20% of the relative abundance of human MTP in HepG2 cells. Radiolabeling of C127 cells with [³⁵S]methionine and [³⁵S]- cysteine followed by immunoprecipitation with anti-MTP antibodies identified a band with an electrophoretic mobility identical to that of authentic mouse MTP. Cotransfection of apoB-41 and the MTP 97-kDa subunit in C127 cells enhanced apoB secretion by 5-fold relative to apoB-41 transfection alone, suggesting that MTP is limiting in these cells. To establish that MTP expression is responsible for apoB-containing lipoprotein assembly in C127 cells, the effects of the MTP inhibitor BMS-200150 were examined. Secretion of apoB-41 by C127 cells was inhibited to the same extent observed in COS-1 cells cotransfected with apoB-41 and MTP.

These results suggest that low MTP expression, and not the expression or overexpression of another known or novel factor(s), is responsible for apoB assembly and secretion in C127 cells and further supports the essential nature of MTP in the biogenesis of apoB-containing lipoproteins. — Sellers, J. A., and G. S. Shelness. Lipoprotein assembly capacity of the mammary tumor-derived cell line C127 is due to the expression of functional microsomal triglyceride transfer protein. J. Lipid Res. 2001. 42: 1897–1904.

Supplementary key words: endoplasmic reticulum, MTP inhibitors, protein disulfide isomerase, protein secretion, very low density lipoprotein

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				P. G. Frank, S. Pavlides, M. W.-C. Cheung, K. Daumer, and M. P. Lisanti Role of caveolin-1 in the regulation of lipoprotein metabolism Am J Physiol Cell Physiol, July 1, 2008; 295(1): C242 - C248. [Abstract] [Full Text] [PDF]

				P. J. Mohler, M.-Y. Zhu, A. M. Blade, A.-J. L. Ham, G. S. Shelness, and L. L. Swift Identification of a Novel Isoform of Microsomal Triglyceride Transfer Protein J. Biol. Chem., September 14, 2007; 282(37): 26981 - 26988. [Abstract] [Full Text] [PDF]

				V. Petit, L. Arnould, P. Martin, M.-C. Monnot, T. Pineau, P. Besnard, and I. Niot Chronic high-fat diet affects intestinal fat absorption and postprandial triglyceride levels in the mouse J. Lipid Res., February 1, 2007; 48(2): 278 - 287. [Abstract] [Full Text] [PDF]

				J. A. Sellers, L. Hou, D. R. Schoenberg, S. R. Batistuzzo de Medeiros, W. Wahli, and G. S. Shelness Microsomal Triglyceride Transfer Protein Promotes the Secretion of Xenopus laevis Vitellogenin A1 J. Biol. Chem., April 8, 2005; 280(14): 13902 - 13905. [Abstract] [Full Text] [PDF]

				C.-M. Li, J. B. Presley, X. Zhang, N. Dashti, B. H. Chung, N. E. Medeiros, C. Guidry, and C. A. Curcio Retina expresses microsomal triglyceride transfer protein: implications for age-related maculopathy J. Lipid Res., April 1, 2005; 46(4): 628 - 640. [Abstract] [Full Text] [PDF]

				J. W. Gallagher, R. B. Weinberg, and G. S. Shelness apoA-IV tagged with the ER retention signal KDEL perturbs the intracellular trafficking and secretion of apoB J. Lipid Res., October 1, 2004; 45(10): 1826 - 1834. [Abstract] [Full Text] [PDF]

				L. Wang and D. M. Small Interfacial properties of amphipathic {beta} strand consensus peptides of apolipoprotein B at oil/water interfaces J. Lipid Res., September 1, 2004; 45(9): 1704 - 1715. [Abstract] [Full Text] [PDF]

				S. L. Larsson, J. Skogsberg, and J. Bjorkegren The Low Density Lipoprotein Receptor Prevents Secretion of Dense ApoB100-containing Lipoproteins from the Liver J. Biol. Chem., January 9, 2004; 279(2): 831 - 836. [Abstract] [Full Text] [PDF]

				G. S. Shelness, L. Hou, A. S. Ledford, J. S. Parks, and R. B. Weinberg Identification of the Lipoprotein Initiating Domain of Apolipoprotein B J. Biol. Chem., November 7, 2003; 278(45): 44702 - 44707. [Abstract] [Full Text] [PDF]

				R. B. Weinberg, V. R. Cook, J. A. Beckstead, D. D. O. Martin, J. W. Gallagher, G. S. Shelness, and R. O. Ryan Structure and Interfacial Properties of Human Apolipoprotein A-V J. Biol. Chem., September 5, 2003; 278(36): 34438 - 34444. [Abstract] [Full Text] [PDF]

				J. A. Sellers, L. Hou, H. Athar, M. M. Hussain, and G. S. Shelness A Drosophila Microsomal Triglyceride Transfer Protein Homolog Promotes the Assembly and Secretion of Human Apolipoprotein B: IMPLICATIONS FOR HUMAN AND INSECT LIPID TRANSPORT AND METABOLISM J. Biol. Chem., May 23, 2003; 278(22): 20367 - 20373. [Abstract] [Full Text] [PDF]