|
Journal of Lipid Research, Vol. 42, 2015-2020, December 2001
Copyright © 2001 by Lipid Research, Inc.
Eicosapentaenoic acid and docosahexaenoic acid modulate MAP kinase (ERK1/ERK2) signaling in human T cells
Anne Denysa,
Aziz Hichamia, and
Naim Akhtar Khana
a UPRES Lipids and Nutrition, Department of Physiology, Faculty of Life Sciences, University of Burgundy, 6 Boulevard Gabriel, Dijon 21000, France
Correspondence to:
Naim Akhtar Khan, To whom correspondence should be addressed., Naim.Khan{at}u-bourgogne.fr (E-mail)
This study was conducted on human Jurkat T cell lines to elucidate the role of EPA and DHA, n-3 PUFA, in the modulation of two mitogen-activated protein (MAP) kinases, that is, extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2). The n-3 PUFA alone failed to induce phosphorylation of ERK1/ERK2. We stimulated the MAP kinase pathway with anti-CD3 antibodies and phorbol 12-myristate 13-acetate (PMA), which act upstream of the MAP kinase (MAPK)/ERK kinase (MEK) as U0126, an MEK inhibitor, abolished the actions of these two agents on MAP kinase activation. EPA and DHA diminished the PMA- and anti-CD3-induced phosphorylation of ERK1/ERK2 in Jurkat T cells. In the present study, PMA acts mainly via protein kinase C (PKC) whereas anti-CD3 antibodies act via PKC-dependent and -independent mechanisms. Furthermore, DHA and EPA inhibited PMA-stimulated PKC enzyme activity. EPA and DHA also significantly curtailed PMA- and ionomycin-stimulated T cell blastogenesis.
Together these results suggest that EPA and DHA modulate ERK1/ERK2 activation upstream of MEK via PKC-dependent and -independent pathways and that these actions may be implicated in n-3 PUFA-induced immunosuppression. Denys, A., A. Hichami, and N. A. Khan. Eicosapentaenoic acid and docosahexaenoic acid modulate MAP kinase (ERK1/ERK2) signaling in human T cells. J. Lipid Res. 2001. 42: 20152020.
Supplementary key words:
T cell receptor, protein kinase C, fatty acids

CiteULike Complore Connotea Del.icio.us Digg Reddit Technorati What's this?
This article has been cited by other articles:

|
 |

|
 |
 
R. Marion-Letellier, M. Butler, P. Dechelotte, R. J Playford, and S. Ghosh
Comparison of cytokine modulation by natural peroxisome proliferator-activated receptor {gamma} ligands with synthetic ligands in intestinal-like Caco-2 cells and human dendritic cells--potential for dietary modulation of peroxisome proliferator-activated receptor {gamma} in intestinal inflammation
Am. J. Clinical Nutrition,
April 1, 2008;
87(4):
939 - 948.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
R. Gorjao, S. M. Hirabara, T. M. de Lima, M. F. Cury-Boaventura, and R. Curi
Regulation of interleukin-2 signaling by fatty acids in human lymphocytes
J. Lipid Res.,
September 1, 2007;
48(9):
2009 - 2019.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Massaro, A. Habib, L. Lubrano, S. D. Turco, G. Lazzerini, T. Bourcier, B. B. Weksler, and R. De Caterina
The omega-3 fatty acid docosahexaenoate attenuates endothelial cyclooxygenase-2 induction through both NADP(H) oxidase and PKC{varepsilon} inhibition
PNAS,
October 10, 2006;
103(41):
15184 - 15189.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Hagiwara, Y. Makita, L. Gu, M. Tanimoto, M. Zhang, S. Nakamura, S. Kaneko, T. Itoh, T. Gohda, S. Horikoshi, et al.
Eicosapentaenoic acid ameliorates diabetic nephropathy of type 2 diabetic KKAy/Ta mice: Involvement of MCP-1 suppression and decreased ERK1/2 and p38 phosphorylation
Nephrol. Dial. Transplant.,
March 1, 2006;
21(3):
605 - 615.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
R. Takahashi, K. Okumura, T. Asai, T. Hirai, H. Murakami, R. Murakami, Y. Numaguchi, H. Matsui, M. Ito, and T. Murohara
Dietary fish oil attenuates cardiac hypertrophy in lipotoxic cardiomyopathy due to systemic carnitine deficiency
Cardiovasc Res,
November 1, 2005;
68(2):
213 - 223.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
C. E. Loscher, E. Draper, O. Leavy, D. Kelleher, K. H. G. Mills, and H. M. Roche
Conjugated Linoleic Acid Suppresses NF-{kappa}B Activation and IL-12 Production in Dendritic Cells through ERK-Mediated IL-10 Induction
J. Immunol.,
October 15, 2005;
175(8):
4990 - 4998.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
H. H. Kim, C. M. Shin, C.-H. Park, K. H. Kim, K. H. Cho, H. C. Eun, and J. H. Chung
Eicosapentaenoic acid inhibits UV-induced MMP-1 expression in human dermal fibroblasts
J. Lipid Res.,
August 1, 2005;
46(8):
1712 - 1720.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. Denys, A. Hichami, and N. A. Khan
n-3 PUFAs modulate T-cell activation via protein kinase C-{alpha} and -{varepsilon} and the NF-{kappa}B signaling pathway
J. Lipid Res.,
April 1, 2005;
46(4):
752 - 758.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Q. Jia, H.-R. Zhou, M. Bennink, and J. J. Pestka
Docosahexaenoic Acid Attenuates Mycotoxin-Induced Immunoglobulin A Nephropathy, Interleukin-6 Transcription, and Mitogen-Activated Protein Kinase Phosphorylation in Mice
J. Nutr.,
December 1, 2004;
134(12):
3343 - 3349.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
D. H. Jho, S. M. Cole, E. M. Lee, and N. J. Espat
Role of Omega-3 Fatty Acid Supplementation in Inflammation and Malignancy
Integr Cancer Ther,
June 1, 2004;
3(2):
98 - 111.
[Abstract]
[PDF]
|
 |
|

|
 |

|
 |
 
C. J Field and P. D Schley
Evidence for potential mechanisms for the effect of conjugated linoleic acid on tumor metabolism and immune function: lessons from n-3 fatty acids
Am. J. Clinical Nutrition,
June 1, 2004;
79(6):
1190S - 1198S.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Madani, A. Hichami, M. Charkaoui-Malki, and N. A. Khan
Diacylglycerols Containing Omega 3 and Omega 6 Fatty Acids Bind to RasGRP and Modulate MAP Kinase Activation
J. Biol. Chem.,
January 9, 2004;
279(2):
1176 - 1183.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
H. Chen, D. Li, G. J Roberts, T. Saldeen, and J. L Mehta
Eicosapentanoic acid inhibits hypoxia-reoxygenation-induced injury by attenuating upregulation of MMP-1 in adult rat myocytes
Cardiovasc Res,
July 1, 2003;
59(1):
7 - 13.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Kew, T. Banerjee, A. M Minihane, Y. E Finnegan, C. M Williams, and P. C Calder
Relation between the fatty acid composition of peripheral blood mononuclear cells and measures of immune cell function in healthy, free-living subjects aged 25-72 y
Am. J. Clinical Nutrition,
May 1, 2003;
77(5):
1278 - 1286.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Bousserouel, A. Brouillet, G. Bereziat, M. Raymondjean, and M. Andreani
Different effects of n-6 and n-3 polyunsaturated fatty acids on the activation of rat smooth muscle cells by interleukin-1{beta}
J. Lipid Res.,
March 1, 2003;
44(3):
601 - 611.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
B. Demmig-Adams and W. W. Adams III
Antioxidants in Photosynthesis and Human Nutrition
Science,
December 13, 2002;
298(5601):
2149 - 2153.
[Abstract]
[Full Text]
[PDF]
|
 |
|
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.
|
Advertisement
Advertisement
|