HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Muñoz, S. Articles by Laguna, J. C. Search for Related Content PubMed PubMed Citation Articles by Muñoz, S. Articles by Laguna, J. C. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 42, 2069-2076, December 2001
Copyright © 2001 by Lipid Research, Inc.

Walnut-enriched diet increases the association of LDL from hypercholesterolemic men with human HepG2 cells

Correspondence to: Juan C. Laguna, at Unidad de Farmacología y Farmacognosia, Facultad de Farmacia, Núcleo Universitario de Pedralbes s/n, Barcelona, 08028, Spain.

In a randomized, cross-over feeding trial involving 10 men with polygenic hypercholesterolemia, a control, Mediterranean-type cholesterol-lowering diet, and a diet of similar composition in which walnuts replaced 35% of energy from unsaturated fat, were given for 6 weeks each. Compared with the control diet, the walnut diet reduced serum total and LDL cholesterol by 4.2% (P = 0.176), and 6.0% (P = 0.087), respectively. No changes were observed in HDL cholesterol, triglycerides, and apolipoprotein A-I levels or in the relative proportion of protein, triglycerides, phospholipids, and cholesteryl esters in LDL particles. The apolipoprotein B level declined in parallel with LDL cholesterol (6.0% reduction). Whole LDL, particularly the triglyceride fraction, was enriched in polyunsaturated fatty acids from walnuts (linoleic and -linolenic acids). In comparison with LDL obtained during the control diet, LDL obtained during the walnut diet showed a 50% increase in association rates to the LDL receptor in human hepatoma HepG2 cells. LDL uptake by HepG2 cells was correlated with -linolenic acid content of the triglyceride plus cholesteryl ester fractions of LDL particles (r² = 0.42, P < 0.05).

Changes in the quantity and quality of LDL lipid fatty acids after a walnut-enriched diet facilitate receptor-mediated LDL clearance and may contribute to the cholesterol-lowering effect of walnut consumption. — Muñoz, S., M. Merlos, D. Zambón, C. Rodríguez, J. Sabaté, E. Ros, and J. C. Laguna. Walnut-enriched diet increases the association of LDL from hypercholesterolemic men with human HepG2 cells. J. Lipid Res. 2001. 42: 2069–2076.

Supplementary key words: fatty acids, LDL receptor, polygenic hypercholesterolemia

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. J. Sheridan, J. N. Cooper, M. Erario, and C. E. Cheifetz Pistachio Nut Consumption and Serum Lipid Levels J. Am. Coll. Nutr., April 1, 2007; 26(2): 141 - 148. [Abstract] [Full Text] [PDF]

				K. G. Jackson, V. Maitin, D. S. Leake, P. Yaqoob, and C. M. Williams Saturated fat-induced changes in Sf 60-400 particle composition reduces uptake of LDL by HepG2 cells J. Lipid Res., February 1, 2006; 47(2): 393 - 403. [Abstract] [Full Text] [PDF]

				J. Mukuddem-Petersen, W. Oosthuizen, and J. C. Jerling A Systematic Review of the Effects of Nuts on Blood Lipid Profiles in Humans J. Nutr., September 1, 2005; 135(9): 2082 - 2089. [Abstract] [Full Text] [PDF]

				E. Ros, I. Nunez, A. Perez-Heras, M. Serra, R. Gilabert, E. Casals, and R. Deulofeu A Walnut Diet Improves Endothelial Function in Hypercholesterolemic Subjects: A Randomized Crossover Trial Circulation, April 6, 2004; 109(13): 1609 - 1614. [Abstract] [Full Text] [PDF]