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Correspondence to:
Gary F. Lewis, at Toronto General Hospital, 200 Elizabeth St., Room EN11-229, Toronto, Ontario, Canada M5G 2C4., gary.lewis{at}uhn.on.ca (E-mail)
Triglyceride (TG) enrichment of high density lipoprotein (HDL), which occurs in hypertriglyceridemic states, significantly enhances the rate at which HDL apolipoprotein (apo)A-I is cleared from the circulation of healthy humans. In the New Zealand White (NZW) rabbit, a species naturally deficient in hepatic lipase (HL), TG enrichment of HDL requires prior lipolytic modification to enhance apoA-I clearance. However, the effect of TG enrichment of HDL on the subsequent clearance of HDL cholesteryl ester (CE) has not previously been examined in vivo. Therefore, we investigated, in the NZW rabbit, the effect of ex vivo TG enrichment of rabbit HDL (by incubation with human very low density lipoprotein) on the clearance of HDL CE and apoA-I radiolabeled with 3H-cholesteryl oleyl ether and with 131I, respectively. In nine experiments, TG enrichment of rabbit HDL resulted in an 87% average increase in HDL TG and a corresponding 31% reduction in HDL CE content.
The calculated apoA-I and CE fractional catabolic rates associated with TG-rich versus fasting HDL tracers were not significantly different (apoA-I: 0.119 ± 0.017 vs. 0.107 ± 0.024 pools per h, P = 0.68; CE: 0.147 ± 0.014 vs. 0.114 ± 0.019 pools per h, P = 0.20). In an animal model deficient in HL, TG enrichment of HDL did not alter the rates of HDL apoA-I or selective CE clearance. Further studies are needed to determine whether, in the presence of HL, TG enrichment of HDL alters selective HDL CE clearance. —Rashid, S., K. D. Uffelman, P. H. R. Barrett, P. Vicini, K. Adeli, and G. F. Lewis. Triglyceride enrichment of HDL does not alter HDL-selective cholesteryl ester clearance in rabbits. J. Lipid Res. 2001. 42: 265;–271.
Supplementary key words:
apolipoprotein A-I, selective cholesterol clearance, high density lipoprotein
Copyright © 2001 by Lipid Research, Inc.
Original Article
Triglyceride enrichment of HDL does not alter HDL-selective cholesteryl ester clearance in rabbits
Shirya Rashida,
Kristine D. Uffelmana,
P. Hugh R. Barrettb,
Paolo Vicinic,
Khosrow Adelid, and
Gary F. Lewisa
a Department of Medicine, Division of Endocrinology, University of Toronto, Toronto, Ontario, Canada M5G 2C4
b Department of Medicine, University of Western Australia, Perth 6847, Australia
c Resource Facility for Population Kinetics, Department of Engineering, University of Washington, Seattle, WA 98195
d Department of Laboratory Medicine and Pathobiology, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada M5G 1X8
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