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Correspondence to:
David J. Orlicky, at the Department of Pathology, University of Colorado Health Sciences Center, Campus Box B216, 4200 East Ninth Avenue, Denver, CO 80262., David.Orlicky{at}UCHSC.edu (E-mail)
3T3-L1 cells offer an excellent model system for studies of differentiation and biochemistry of fat cells. However, these cells are limited in their utility by the low efficiency with which DNA can be introduced by transfection. Gene delivery by viral vectors, particularly adenovirus, has proven a powerful means for introduction of genes into certain cell types. Furthermore, adenovirus transduction has been used to study mechanisms involved in the differentiation of 3T3-L1 cells into mature fat cells.
We show in this study that 3T3-L1 cells are inefficiently transduced by adenovirus. The potential advantages offered by adenovirus transduction led us to examine methods designed to enhance transduction of 3T3-L1 cells by adenovirus. Of these methods, polylysine-mediated enhancement demonstrates considerable promise because it permits up to 100% of cells to be transduced and because it does not inhibit differentiation of 3T3-L1 cells. — Orlicky D. J., and J. Schaack. Adenovirus transduction of 3T3-L1 cells. J. Lipid Res. 2001. 42: 460;–466.
Supplementary key words:
adenoviral vectors, adipocyte lipid homeostasis, transduction efficiency
Copyright © 2001 by Lipid Research, Inc.
Methods
Adenovirus transduction of 3T3-L1 cells
David J. Orlickya,c,e and
Jerome Schaackb,c,d,e
a Department of Pathology, University of Colorado Health Sciences Center, Denver, CO 80262
b Department of Microbiology, University of Colorado Health Sciences Center, Denver, CO 80262
c University of Colorado Cancer Center, University of Colorado Health Sciences Center, Denver, CO 80262
d Program in Molecular Biology, University of Colorado Health Sciences Center, Denver, CO 80262
e Biomedical Sciences Program, University of Colorado Health Sciences Center, Denver, CO 80262
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