J. Lipid Res.
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Journal of Lipid Research, Vol. 42, 873-885, May 2001
Copyright © 2001 by Lipid Research, Inc.

Methods

Confirmation of minor components of less polar neutral and acidic glycolipids in monkey brain tissue

Tamotsu Taketomia, Eiko Sugiyamaa, Kei-ichi Uemuraa, Atsushi Haraa, Hiroya Hidakab, Minoru Tozukab, Tetsuo Nakabayashib, and Tsutomu Katsuyamab
a Division of Neuronal Aging, Shinshu University School of Medicine, Matsumoto 390-8621, Japan
b Research Center on Aging and Adaptation, and Central Clinical Laboratories, Shinshu University School of Medicine, Matsumoto 390-8621, Japan

Correspondence to: Tamotsu Taketomi, at Shinshu Clinical Lipid Research Institute, 6-15-13 Arigasaki, Matsumoto 390-0861, Japan., jz2t-tktm{at}asahi-net.or.jp (E-mail)

Crude glycolipids, prepared without alkali treatment in advance, were separated into neutral and acidic glycolipids by DEAE-Sephadex A-25 (acetate form) column chromatography. Each glycolipid was further fractionated by a Silica gel 60-column chromatography. By matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with delayed ion extraction (DE MALDI-TOF MS) of the intact glycolipid fractions, the less polar neutral glycolipids were found to contain alkali-labile ester cerebrosides and Galb-1-Diradylglycerols, whereas the less polar acidic glycolipids were found to contain alkali-labile ester sulfatide, HSO3-3Gal-1-Diradylglycerols, and novel alkali-stable plasmalo-sulfatides and ester or plasmalo HSO3-3Galb-1-Diradylglycerols as minor components of glycolipids in monkey brain tissue.

In conclusion, minor components of less polar neutral and acidic glycolipids in monkey brain tissue were confirmed as ester cerebrosides, Galb-1-Diradylglycerols, ester sulfatides, HSO3-3Galb-1-Diradylglycerols, and novel plasmalo-sulfatides and ester or plasmalo HSO3-3Galb-1-Diradylglycerols by DE MALDI-TOF MS.—Taketomi, T., E. Sugiyama, K-i. Uemura, A. Hara, H. Hidaka, M. Tozuka, T. Nakabayashi, and T. Katsuyama. Confirmation of minor components of less polar neutral and acidic glycolipids in monkey brain tissue. J. Lipid Res. 2001. 42: 873;–885.

Supplementary key words: Galß-1-Diradylglycerols, HSO3-3Galß-1-Diradylglycerols


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