|
 |
|
|||||||
|
|||||||||
Correspondence to:
David Y. Hui, To whom correspondence should be addressed., Huidy{at}email.uc.edu (E-mail)
The current study used the human Caco-2 cell line and mouse intestine to explore the topology of expression of the class B type I scavenger receptor (SR-BI) in intestinal cells. Results showed that intestinal cells expressed only the SR-BI isoform with little or no expression of the SR-BII variant. The expression of SR-BI in Caco-2 cells is differentiation dependent, with little or no expression in preconfluent undifferentiated cells. Analysis of Caco-2 cells cultured in Transwell porous membranes revealed the presence of SR-BI on both the apical and basolateral cell surface. Immunoblot analysis of mouse intestinal cell extracts demonstrated a gradation of SR-BI expression along the gastrocolic axis of the intestine, with the highest level of expression in the proximal intestine and decreasing to minimal expression levels in the distal intestine. Immunofluorescence studies with SR-BI-specific antibodies also confirmed this expression pattern. Importantly, the immunofluorescence studies also revealed that SR-BI immunoreactivity was most intense in the apical membrane of the brush border in the duodenum. The crypt cells did not show any reactivity with SR-BI antibodies. The localization of SR-BI in the jejunum was found to be different from that observed in the duodenum. SR-BI was present on both apical and basolateral surfaces of the jejunum villus. Localization of SR-BI in the ileum was also different, with little SR-BI detectable on either apical or basolateral membranes.
Taken together, these results suggest that SR-BI has the potential to serve several functions in the intestine. The localization of SR-BI on the apical surface of the proximal intestine is consistent with the hypothesis of its possible role in dietary cholesterol absorption, whereas SR-BI present on the basolateral surface of the distal intestine suggests its possible involvement in intestinal lipoprotein uptake. — Cai, S. F., R. J. Kirby, P. N. Howles, and D. Y. Hui. Differentiation-dependent expression and localization of the class B type I scavenger receptor in intestine. J. Lipid Res. 2001. 42: 902;–909.
Supplementary key words:
cholesterol absorption, lipid transport, lipoprotein receptor
Copyright © 2001 by Lipid Research, Inc.
Differentiation-dependent expression and localization of the class B type I scavenger receptor in intestine
Sheng F. Caia,
R. Jason Kirbya,
Philip N. Howlesa, and
David Y. Huia
a Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, OH 45267
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Ghoshal, J. Witta, J. Zhong, W. de Villiers, and E. Eckhardt Chylomicrons promote intestinal absorption of lipopolysaccharides J. Lipid Res., January 1, 2009; 50(1): 90 - 97. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Seino, T. Miki, H. Kiyonari, T. Abe, W. Fujimoto, K. Kimura, A. Takeuchi, Y. Takahashi, Y. Oiso, T. Iwanaga, et al. Isx Participates in the Maintenance of Vitamin A Metabolism by Regulation of {beta}-Carotene 15,15'-Monooxygenase (Bcmo1) Expression J. Biol. Chem., February 22, 2008; 283(8): 4905 - 4911. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Mee, J. Grove, H. J. Harris, K. Hu, P. Balfe, and J. A. McKeating Effect of Cell Polarization on Hepatitis C Virus Entry J. Virol., January 1, 2008; 82(1): 461 - 470. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. During and E. H. Harrison Mechanisms of provitamin A (carotenoid) and vitamin A (retinol) transport into and out of intestinal Caco-2 cells J. Lipid Res., October 1, 2007; 48(10): 2283 - 2294. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Y. Choi, A. I. Romer, M. Hu, M. Lepourcelet, A. Mechoor, A. Yesilaltay, M. Krieger, P. A. Gray, and R. A. Shivdasani A dynamic expression survey identifies transcription factors relevant in mouse digestive tract development Development, October 15, 2006; 133(20): 4119 - 4129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. Sane, D. Sinnett, E. Delvin, M. Bendayan, V. Marcil, D. Menard, J.-F. Beaulieu, and E. Levy Localization and role of NPC1L1 in cholesterol absorption in human intestine J. Lipid Res., October 1, 2006; 47(10): 2112 - 2120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Sun, E. R. M. Eckhardt, S. Shetty, D. R. van der Westhuyzen, and N. R. Webb Quantitative analysis of SR-BI-dependent HDL retroendocytosis in hepatocytes and fibroblasts J. Lipid Res., August 1, 2006; 47(8): 1700 - 1713. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Briand, S. Serisier, M. Krempf, B. Siliart, T. Magot, K. Ouguerram, and P. Nguyen Atorvastatin Increases Intestinal Cholesterol Absorption in Dogs J. Nutr., July 1, 2006; 136(7): 2034S - 2036S. [Full Text] [PDF]
|
|
|
|
|
|
F. Bietrix, D. Yan, M. Nauze, C. Rolland, J. Bertrand-Michel, C. Comera, S. Schaak, R. Barbaras, A. K. Groen, B. Perret, et al. Accelerated Lipid Absorption in Mice Overexpressing Intestinal SR-BI J. Biol. Chem., March 17, 2006; 281(11): 7214 - 7219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Temel, R. G. Lee, K. L. Kelley, M. A. Davis, R. Shah, J. K. Sawyer, M. D. Wilson, and L. L. Rudel Intestinal cholesterol absorption is substantially reduced in mice deficient in both ABCA1 and ACAT2 J. Lipid Res., November 1, 2005; 46(11): 2423 - 2431. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. During, H. D. Dawson, and E. H. Harrison Carotenoid Transport Is Decreased and Expression of the Lipid Transporters SR-BI, NPC1L1, and ABCA1 Is Downregulated in Caco-2 Cells Treated with Ezetimibe J. Nutr., October 1, 2005; 135(10): 2305 - 2312. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Deforges, A. Evlashev, M. Perret, M. Sodoyer, S. Pouzol, J.-Y. Scoazec, B. Bonnaud, O. Diaz, G. Paranhos-Baccala, V. Lotteau, et al. Expression of hepatitis C virus proteins in epithelial intestinal cells in vivo J. Gen. Virol., September 1, 2004; 85(9): 2515 - 2523. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Rhainds, P. Bourgeois, G. Bourret, K. Huard, L. Falstrault, and L. Brissette Localization and regulation of SR-BI in membrane rafts of HepG2 cells J. Cell Sci., July 1, 2004; 117(15): 3095 - 3105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ritsch, I. Tancevski, W. Schgoer, C. Pfeifhofer, R. Gander, P. Eller, B. Foeger, U. Stanzl, and J. R. Patsch Molecular characterization of rabbit scavenger receptor class B types I and II: portal to central vein gradient of expression in the liver J. Lipid Res., February 1, 2004; 45(2): 214 - 222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Cai, E. R. M. Eckhardt, W. Shi, Z. Zhao, M. Nasser, W. J. S. de Villiers, and D. R. van der Westhuyzen Scavenger receptor class B type I reduces cholesterol absorption in cultured enterocyte CaCo-2 cells J. Lipid Res., February 1, 2004; 45(2): 253 - 262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Levy, D. Menard, I. Suc, E. Delvin, V. Marcil, L. Brissette, L. Thibault, and M. Bendayan Ontogeny, immunolocalisation, distribution and function of SR-BI in the human intestine J. Cell Sci., January 15, 2004; 117(2): 327 - 337. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G H Hansen, L-L Niels-Christiansen, L Immerdal, and E M Danielsen Scavenger receptor class B type I (SR-BI) in pig enterocytes: trafficking from the brush border to lipid droplets during fat absorption Gut, October 1, 2003; 52(10): 1424 - 1431. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Bruneau, S. Richard, F. Silvy, A. Verine, and D. Lombardo Lectin-like Ox-LDL Receptor Is Expressed in Human INT-407 Intestinal Cells: Involvement in the Transcytosis of Pancreatic Bile Salt-dependent Lipase Mol. Biol. Cell, July 1, 2003; 14(7): 2861 - 2875. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Rigotti, H. E. Miettinen, and M. Krieger The Role of the High-Density Lipoprotein Receptor SR-BI in the Lipid Metabolism of Endocrine and Other Tissues Endocr. Rev., June 1, 2003; 24(3): 357 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. B. Loest, S. K. Noh, and S. I. Koo Green Tea Extract Inhibits the Lymphatic Absorption of Cholesterol and {alpha}-Tocopherol in Ovariectomized Rats J. Nutr., June 1, 2002; 132(6): 1282 - 1288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Rong, S. Ramachandran, M. Penumetcha, N. Khan, and S. Parthasarathy Dietary oxidized fatty acids may enhance intestinal apolipoprotein A-I production J. Lipid Res., April 1, 2002; 43(4): 557 - 564. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |