J. Lipid Res.
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Journal of Lipid Research, Vol. 42, 910-915, June 2001
Copyright © 2001 by Lipid Research, Inc.

Construction of stable coxsackievirus and adenovirus receptor-expressing 3T3-L1 cells

David J. Orlickya,e,g, James DeGregorib,c,e,f,g, and Jerome Schaackd,e,f,g
a Departments of Pathology, University of Colorado Health Sciences Center, Denver, CO 80262
b Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, Denver, CO 80262
c Pediatrics, University of Colorado Health Sciences Center, Denver, CO 80262
d Microbiology, University of Colorado Health Sciences Center, Denver, CO 80262
e University of Colorado Cancer Center, University of Colorado Health Sciences Center, Denver, CO 80262
f Program in Molecular Biology, University of Colorado Health Sciences Center, Denver, CO 80262
g Biomedical Sciences Program, University of Colorado Health Sciences Center, Denver, CO 80262

Correspondence to: David J. Orlicky, at the University of Colorado Health Sciences Center, Department of Pathology, Campus Box B216 4200, East Ninth Avenue, Denver, CO 80262., David.Orlicky{at}UCHSC.edu (E-mail)

3T3-L1 cells have been used as a model to study the differentiation and physiology of adipocytes. Exogenous expression of proteins in these cells offers the prospect of understanding the protein's function(s) in adipose tissue. Viral vectors, in particular, adenovirus, have proven to be a powerful means for introduction of genes into many cell types. However, we have previously shown that 3T3-L1 cells are inefficiently transduced by adenovirus (Orlicky, D. J., and J. Schaack. 2001. J. Lipid Res. 42: 460–466). To overcome the inefficient transduction, we have stably introduced the gene-encoding coxsackie and adenovirus receptor (CAR), which was modified by deletion of the region encoding the cytoplasmic tail, into 3T3-L1 cells.

3T3-L1 CAR{Delta}1 cells are transduced approximately 100-fold more efficiently than parental 3T3-L1 cells. 3T3-L1 CAR{Delta}1 cells should prove to be a useful tool for examination of exogenous protein expression in fat cells.—Orlicky, D. J., J. DeGregori, and J. Schaack. Construction of stable coxsackievirus and adenovirus receptor-expressing 3T3-L1 cells. J. Lipid Res. 2001. 42: 910–915.

Supplementary key words: 3T3-L1, adenovirus, transduction, adipose, adipocyte


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