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Correspondence to:
Koji Uchida, To whom correspondence should be addressed., uchidak{at}agr.nagoya-u.ac.jp (E-mail)
The accumulation of fluorescent age pigment or lipofuscin is a frequently observed age-associated cellular alteration in a variety of postmitotic cells of many species. These pigments are observed within granules composed, in part, of damaged protein and lipid. Modification of various biomolecules by aldehyde products of lipid peroxidation is believed to contribute to lipofuscin and ceroid formation. In the present study, we raised a monoclonal antibody (MAb 1F83) directed to the malondialdehyde-modified protein and identified a lipofuscin-like dihydropyridine fluorophore as the major epitope. This antibody was used to conclusively demonstrate that the fluorophore forms on oxidatively modified low density lipoproteins. In addition, we demonstrated that the materials immunoreactive to MAb 1F83 indeed constituted the atherosclerotic lesions, in which intense positivity was associated primarily with macrophage-derived foam cells.
The results of this study suggest that the reaction between the lipid peroxidation-derived aldehyde and primary amino groups of protein might represent a process common to the formation of the lipofuscin-like fluorophore during aging and its related diseases. Yamada, S., S. Kumazawa, T. Ishii, T. Nakayama, K. Itakura, N. Shibata, M. Kobayashi, K. Sakai, T. Osawa, and K. Uchida. Immunochemical detection of a lipofuscin-like fluorophore derived from malondialdehyde and lysine. J. Lipid Res. 2001. 42: 1187;1196.
Supplementary key words:
lipid peroxidation, protein modification, dihydropyridine, monoclonal antibody
Copyright © 2001 by Lipid Research, Inc.
Original Article
Immunochemical detection of a lipofuscin-like fluorophore derived from malondialdehyde and lysine
Satoshi Yamadaa,
Shigenori Kumazawab,
Takeshi Ishiib,
Tsutomu Nakayamab,
Koichi Itakurac,
Noriyuki Shibatad,
Makio Kobayashid,
Kensuke Sakaie,
Toshihiko Osawae, and
Koji Uchidae
a Tsukuba Research Laboratory, NOF Company, Tsukuba 300-2635, Japan
b Department of Food and Nutritional Sciences, University of Shizuoka, Shizuoka 422-8529, Japan
c Faculty of Education, Aichi University of Education, Kariya 448, Japan
d Department of Pathology, Tokyo Women's Medical University, Tokyo 162-8666, Japan
e Laboratory of Food and Biodynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
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