HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chochina, S. V. Articles by Wood, W. G. Search for Related Content PubMed PubMed Citation Articles by Chochina, S. V. Articles by Wood, W. G. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 42, 1292-1297, August 2001
Copyright © 2001 by Lipid Research, Inc.

Original Article

Amyloid ß-peptide_1-40 increases neuronal membrane fluidity: role of cholesterol and brain region

Correspondence to: W. G. Wood, at: VA Medical Center, GRECC, 11G, Minneapolis, MN 55417., Woodx002{at}tc.umn.edu (E-mail)

There is increasing evidence of an interaction between cholesterol dynamics and Alzheimer's disease (AD), and amyloid ß-peptide may play an important role in this interaction. Aß destabilizes brain membranes and this action of Aß may be dependent on the amount of membrane cholesterol. We tested this hypothesis by examining effects of Aß_1-40 on the annular fluidity (i.e., lipid environment adjacent to proteins) and bulk fluidity of rat synaptic plasma membranes (SPM) of the cerebral cortex, cerebellum, and hippocampus using the fluorescent probe pyrene and energy transfer. Amounts of cholesterol and phospholipid of SPM from each brain region were determined. SPM of the cerebellum were significantly more fluid as compared with SPM of the cerebral cortex and hippocampus. Aß significantly increased (P 0.01) annular and bulk fluidity in SPM of cerebral cortex and hippocampus. In contrast, Aß had no effect on annular fluidity and bulk fluidity of SPM of cerebellum. The amounts of cholesterol in SPM of cerebral cortex and hippocampus were significantly higher (P 0.05) than amount of cholesterol in SPM of cerebellum. There was significantly less (P 0.05) total phospholipid in cerebellar SPM as compared with SPM of cerebral cortex.

Neuronal membranes enriched in cholesterol may promote accumulation of Aß by hydrophobic interaction, and such an interpretation is consistent with recent studies showing that soluble Aß can act as a seed for fibrillogenesis in the presence of cholesterol. — Chochina, S. V., N. A. Avdulov, U. Igbavboa, J. P. Cleary, E. O. O'Hare, and W. G. Wood. Amyloid ß-peptide_1-40 increases neuronal membrane fluidity: role of cholesterol and brain region. J. Lipid Res. 2001. 42: 1292;–1297.

Supplementary key words: Alzheimer's disease, lipids, neuron, phospholipid

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				O. Zamir and M. P. Charlton Cholesterol and synaptic transmitter release at crayfish neuromuscular junctions J. Physiol., February 15, 2006; 571(1): 83 - 99. [Abstract] [Full Text] [PDF]

				U. Igbavboa, J. M. Pidcock, L. N. A. Johnson, T. M. Malo, A. E. Studniski, S. Yu, G. Y. Sun, and W. G. Wood Cholesterol Distribution in the Golgi Complex of DITNC1 Astrocytes Is Differentially Altered by Fresh and Aged Amyloid beta -Peptide-(1-42) J. Biol. Chem., May 2, 2003; 278(19): 17150 - 17157. [Abstract] [Full Text] [PDF]

				N. ARISPE and M. DOH Plasma membrane cholesterol controls the cytotoxicity of Alzheimer's disease A{beta}P (1-40) and (1-42) peptides FASEB J, October 1, 2002; 16(12): 1526 - 1536. [Abstract] [Full Text] [PDF]

				S. W. Chiu, E. Jakobsson, R. J. Mashl, and H. L. Scott Cholesterol-Induced Modifications in Lipid Bilayers: A Simulation Study Biophys. J., October 1, 2002; 83(4): 1842 - 1853. [Abstract] [Full Text] [PDF]