HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pérusse, M. Articles by Lamarche, B. Search for Related Content PubMed PubMed Citation Articles by Pérusse, M. Articles by Lamarche, B. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 42, 1331-1334, August 2001
Copyright © 2001 by Lipid Research, Inc.

methods

A new method for HDL particle sizing by polyacrylamide gradient gel electrophoresis using whole plasma

Correspondence to: Benoît Lamarche, To whom correspondence should be addressed., benoit.lamarche{at}crchul.ulaval.ca (E-mail)

Low plasma levels of HDL cholesterol have been associated with an increased risk of coronary heart disease. HDL particles are heterogeneous with respect to size and apolipoprotein content. The objective of the present study was to develop a method to generate lipid-stainable calibrators that would allow the assessment of HDL particle size from whole plasma, using polyacrylamide gradient gel electrophoresis (PAGGE). Lipid-stainable HDL calibrators were obtained by subjecting isolated red blood cells to hemolysis either by freezing at - 20 or - 80°C overnight or by rapid exposure to liquid nitrogen and mixing of the hemolysis products with plasma aliquots. All three methods were highly reproducible in producing Sudan black lipid-stainable HDL calibrators ranging from 75 to 200 Å. The assessment of HDL particle size with these lipid-stainable HDL calibrators was also highly reproducible, with a coefficient of variation below 5.5%.

These lipid-stainable HDL calibrators simplify the assessment of HDL particle size by PAGGE using whole plasma, without the need for costly, time-consuming ultracentrifugation procedures. — Pérusse, M., A. Pascot, J-P. Després, C. Couillard, and B. Lamarche. A new method for HDL particle sizing by polyacrylamide gradient gel electrophoresis using whole plasma. J. Lipid Res. 2001. 42: 1331;–1334.

Supplementary key words: HDL particle size, hemolysis, lipid staining

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				H. Watanabe, S. Soderlund, A. Soro-Paavonen, A. Hiukka, E. Leinonen, C. Alagona, R. Salonen, T.-P. Tuomainen, C. Ehnholm, M. Jauhiainen, et al. Decreased High-Density Lipoprotein (HDL) Particle Size, Pre{beta}-, and Large HDL Subspecies Concentration in Finnish Low-HDL Families: Relationship With Intima-Media Thickness Arterioscler. Thromb. Vasc. Biol., April 1, 2006; 26(4): 897 - 902. [Abstract] [Full Text] [PDF]

				S. Desroches, M.-E. Paradis, M. Perusse, W. R. Archer, J. Bergeron, P. Couture, N. Bergeron, and B. Lamarche Apolipoprotein A-I, A-II, and VLDL-B-100 metabolism in men: comparison of a low-fat diet and a high-monounsaturated fatty acid diet J. Lipid Res., December 1, 2004; 45(12): 2331 - 2338. [Abstract] [Full Text] [PDF]

				I. L. Ruel, P. Couture, J. S. Cohn, A. Bensadoun, M. Marcil, and B. Lamarche Evidence that hepatic lipase deficiency in humans is not associated with proatherogenic changes in HDL composition and metabolism J. Lipid Res., August 1, 2004; 45(8): 1528 - 1537. [Abstract] [Full Text] [PDF]

				E. S. Lima and R. C. Maranhao Rapid, Simple Laser-Light-Scattering Method for HDL Particle Sizing in Whole Plasma Clin. Chem., June 1, 2004; 50(6): 1086 - 1088. [Full Text] [PDF]

				M.-E. Paradis, P. Couture, Y. Bosse, J.-P. Despres, L. Perusse, C. Bouchard, M.-C. Vohl, and B. Lamarche The T111I mutation in the EL gene modulates the impact of dietary fat on the HDL profile in women J. Lipid Res., October 1, 2003; 44(10): 1902 - 1908. [Abstract] [Full Text] [PDF]

				I. L. Ruel, P. Couture, C. Gagne, Y. Deshaies, J. Simard, R. A. Hegele, and B. Lamarche Characterization of a novel mutation causing hepatic lipase deficiency among French Canadians J. Lipid Res., August 1, 2003; 44(8): 1508 - 1514. [Abstract] [Full Text] [PDF]

				A. Pascot, I. Lemieux, D. Prud'homme, A. Tremblay, A. Nadeau, C. Couillard, J. Bergeron, B. Lamarche, and J.-P. Despres Reduced HDL particle size as an additional feature of the atherogenic dyslipidemia of abdominal obesity J. Lipid Res., December 1, 2001; 42(12): 2007 - 2014. [Abstract] [Full Text] [PDF]