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Journal of Lipid Research, Vol. 42, 1501-1508, September 2001
Copyright © 2001 by Lipid Research, Inc.

Methods

Lipid analysis of human HDL and LDL by MALDI-TOF mass spectrometry and ³¹P-NMR

, Marijana Petkovi

Correspondence to: Jürgen Schiller, To whom correspondence should be addressed., schij{at}medizin.uni-leipzig.de (E-mail)

The analysis of HDL and LDL is important for the further understanding of atherosclerosis because changes of the protein and lipid moieties occur under pathological conditions. Because destruction of lipids leads to the formation of well-defined products such as lysophospholipids or chlorohydrins, methods that allow their fast and reliable determination would be useful. In this study, matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) was applied for the analysis of the lipid composition of human lipoproteins. These data were compared with high resolution ³¹P-NMR spectroscopy. Differences between LDL and HDL in sphingomyelin and phosphatidylcholine content could be monitored by NMR and mass spectrometry, and differences with respect to the extraction efficiency were found by MALDI-TOF MS. Additionally, treatment of LDL with hypochlorite and phospholipase A₂ resulted in marked changes (formation of chlorohydrines and lysolipids). Lysophosphatidylcholines were detectable by both methods, whereas MALDI-TOF MS failed to detect chlorohydrines of phospholipids.

We conclude that MALDI-TOF MS provides rapidly a reliable lipid profile of lipoproteins. However, a previous lipid separation must be performed to detect lipid oxidation products. NMR can be directly applied, but suffers from lower sensitivity, and provides only limited information on fatty acid composition. — Schiller, J., O. Zschörnig, M. Petkovi, M. Müller, J. Arnhold, and K. Arnold. Lipid analysis of human HDL and LDL by MALDI-TOF mass spectrometry and ³¹P-NMR. J. Lipid Res. 2001. 42: 1501;–1508.

Supplementary key words: MALDI-TOF MS, lysolipids, chlorohydrine

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