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Journal of Lipid Research, Vol. 43, 132-140, January 2002
Copyright © 2002 by Lipid Research, Inc.

Lipid-protein stoichiometries in a crystalline biological membrane: NMR quantitative analysis of the lipid extract of the purple membrane

Correspondence to: Angela Corcelli, at Dipartimento di Fisiologia Generale ed Ambientale, Università degli Studi di Bari, Via Amendola 165/a, 70126, Bari, Italy., a.corcelli{at}biologia.uniba.it (E-mail)

The lipid/protein stoichiometries of a naturally crystalline biological membrane, the purple membrane (PM) of Halobacterium salinarum, have been obtained by a combination of ³¹P- and ¹H-NMR analyses of the lipid extract. In total, 10 lipid molecules per retinal were found to be present in the PM lipid extract: 2–3 molecules of phosphatidylglycerophosphate methyl ester (PGP-Me), 3 of glycolipid sulfate, 1 of phosphatidylglycerol, 1 of archaeal glycocardiolipin (GlyC), 2 of squalene plus minor amounts of phosphatidylglycerosulfate (PGS) and bisphosphatidylglycerol (archaeal cardiolipin) (BPG) and a negligible amount of vitamin MK8.

The novel data of the present study are necessary to identify the lipids in the electron density map, and to shed light on the structural relationships of the lipid and protein components of the PM. — Corcelli, A., V. M. T. Lattanzio, G. Mascolo, P. Papadia, and F. Fanizzi. Lipid-protein stoichiometries in a crystalline biological membrane: NMR quantitative analysis of the lipid extract of the purple membrane. J. Lipid Res. 2002. 43: 132–140.

Supplementary key words: archaeal lipids, archaeal cardiolipin, glycocardiolipin, purple membrane, bacteriorhodopsin, nuclear magnetic resonance lipid analyses, lipid/protein molar ratio, thin-layer chromatography

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