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J. Lipid Res.
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Journal of Lipid Research, Vol. 43, 69-73, January 2002
Copyright © 2002 by Lipid Research, Inc.

A synthetic low density lipoprotein particle capable of supporting U937 proliferation in vitro

G. Bailliea, M. D. Owensa, and G. W. Halberta
a Department of Pharmaceutical Sciences, Strathclyde Institute for Biomedical Sciences, University of Strathclyde, 27 Taylor Street, Glasgow, G4 0NR, United Kingdom

Correspondence to: G. W. Halbert, To whom correspondence should be addressed., g.w.halbert{at}strath.ac.uk (E-mail)

A synthetic LDL (sLDL) has been prepared by combining a lipid microemulsion with amphipathic peptides containing the apoprotein B receptor domain. The biological properties of sLDL have been investigated using the U937 in vitro cell proliferation assay. sLDL exhibits a concentration dependent and saturable stimulation of U937 proliferation. By utilizing different amphipathic peptides, variable proliferation is achieved, indicating a specific interaction between sLDL and the U937 LDL receptor are possible. U937 proliferation is reduced by the addition of an anti-LDL receptor antibody, indicating that sLDL is assimilated via the LDL receptor pathway.

The behavior of sLDL mimics that of native LDL, and this approach represents a viable technique for the production of an sLDL particle on a large scale for research and general application. — Baillie, G., M. D. Owens, and G. W. Halbert. A synthetic low density lipoprotein particle capable of supporting U937 proliferation in vitro. J. Lipid Res. 2002. 43: 69–73.

Supplementary key words: U937, low density lipoprotein, amphipathic peptide, apoB, synthetic LDL, phosphate-buffered saline, dimethyl sulfoxide


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