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Journal of Lipid Research, Vol. 43, 187-197, February 2002
Copyright © 2002 by Lipid Research, Inc.

Interaction of apolipoprotein A-I in three different conformations with palmitoyl oleoyl phosphatidylcholine vesicles

Correspondence to: Ana Jonas, at Department of Biochemistry, College of Medicine at Urbana-Champaign, University of Illinois, 506 Matthews Ave., Urbana, IL 61801., a-jonas{at}uiuc.edu (E-mail)

Interactions of apolipoprotein A-I (apoA-I) with cell membranes appear to be important in the initial steps of reverse cholesterol transport. The objective of this work was to examine the effect of three distinct conformations of apoA-I (lipid-free and in 78 Å or 96 Å reconstituted high density lipoproteins, rHDL) on its ability to bind to, and abstract lipids from, palmitoyl oleoyl phosphatidylcholine membrane vesicles (small unilamellar vesicles, SUV, and giant unilamellar vesicles, GUV). The molecular interactions were observed by two-photon fluorescence microscopy, and the binding parameters were quantified by gel-permeation chromatography or isothermal titration microcalorimetry. Rearrangement of apoA-I-containing particles after exposure to SUVs was examined by native gel electrophoresis. The results indicate that lipid-free apoA-I binds reversibly, with high affinity, to the vesicles but does not abstract a significant amount of lipid nor perturb the vesicle structure. The 96 Å rHDL, where all the amphipathic helices of apoA-I are saturated with lipid within the particles, do not bind to vesicles or perturb their structure. In contrast, the 78 Å rHDL have a region of apoA-I, corresponding to a few amphipathic helical segments, which is available for external or internal phospholipid binding. These particles bind to vesicles with measurable affinity (lower than lipid-free apoA-I), abstract lipids from the membranes, and form particles of larger diameters, including 96 Å rHDL.

We conclude that the conformation of apoA-I regulates its binding affinity for phospholipid membranes and its ability to abstract lipids from the membranes. — Tricerri, M. A., S. A. Sanchez, C. Arnulphi, D. M. Durbin, E. Gratton, and A. Jonas. Interaction of apolipoprotein A-I in three different conformations with palmitoyl oleoyl phosphatidylcholine vesicles. J. Lipid Res. 2002. 43: 187–197.

Supplementary key words: vesicle interactions, small unilamellar vesicles, giant unilamellar vesicles, remodeling of HDL

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