| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Correspondence to:
Alexandros D. Tselepis, To whom correspondence should be addressed., atselep{at}cc.uoi.gr (E-mail)
Platelet-activating factor-acetylhydrolase (PAF-AH) is a lipoprotein-associated phospholipase A2 capable of hydrolyzing platelet-activating factor (PAF) and oxidatively modified phospholipids. We studied the plasma- and lipoprotein-associated PAF-AH activity in patients with primary hypercholesterolemia. Thirty-eight unrelated patients with heterozygous familial hypercholesterolemia (HeteroFH), five patients with homozygous FH (HomoFH), and 33 patients with primary non-FH hypercholesterolemia (NonFH) participated in the study. In all patient groups the plasma PAF-AH activity was significantly elevated compared with 33 normolipidemic controls, the HomoFH having the highest and the NonFH patients showing the lowest enzyme activity. Gradient ultracentrifugation studies showed that this increase is not only due to the elevation in the plasma LDL but also to the increase in the PAF-AH activity associated with each LDL subfraction, being more profound in the small-dense LDL-5. Unlike LDL, no difference in the HDL-associated PAF-AH activity was observed among all groups. Consequently, an altered distribution of enzyme activity among apolipoprotein B (apoB)- and apolipoprotein A-I (apoA-I)-containing lipoproteins is observed in hypercholesterolemic patients, resulting in a significant decrease in the ratio of the HDL-associated PAF-AH to the total plasma enzyme activity compared with controls. This reduction is proportional to the increase of the plasma LDL-cholesterol (LDL-C) levels and consequently to the severity of the hypercholesterolemia.
Thus, the ratio of HDL-associated PAF-AH-total plasma enzyme activity may be useful as a potential marker of atherogenicity in subjects with primary hypercholesterolemia. — Tsimihodimos, V., S-A. P. Karabina, A. P. Tambaki, E. Bairaktari, G. Miltiadous, J. A. Goudevenos, M. A. Cariolou, M. J. Chapman, A. D. Tselepis, and M. Elisaf. Altered distribution of platelet-activating factor-acetylhydrolase activity between LDL and HDL as a function of the severity of hypercholesterolemia. J. Lipid Res. 2002. 43: 256–263.
Supplementary key words:
familial hypercholesterolemia, low density lipoprotein, lipoprotein subspecies, high density lipoprotein, PAF-acetylhydrolase, polygenic hypercholesterolemia
Copyright © 2002 by Lipid Research, Inc.
Altered distribution of platelet-activating factor- acetylhydrolase activity between LDL and HDL as a function of the severity of hypercholesterolemia
Vasilis Tsimihodimosa,
Sonia-Athena P. Karabinad,
Afroditi P. Tambakid,
Eleni Bairaktaric,
George Miltiadousa,
John A. Goudevenosb,
Marios A. Carioloue,
M. John Chapmanf,
Alexandros D. Tselepisd, and
Moses Elisafa
a Departments of Internal Medicine, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece
b Cardiology, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece
c Laboratory of Biological Chemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece
d Medical School, and Laboratory of Biochemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece
e Molecular Genetics Unit B-DNA Identification Laboratory, The Cyprus Institute of Neurology and Genetics, Nicosia, Cyprus
f Institut National de la Santé et de la Recherché, Unité 551, Hôpital de la Pitié, 83 Bd de l'Hôpital, 75651 Paris Cedex 13, France
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. A. Gardner, E. C. Reichert, M. K. Topham, and D. M. Stafforini Identification of a Domain That Mediates Association of Platelet-activating Factor Acetylhydrolase with High Density Lipoprotein J. Biol. Chem., June 20, 2008; 283(25): 17099 - 17106. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Robins, D. Collins, J. J. Nelson, H. E. Bloomfield, and B. F. Asztalos Cardiovascular Events With Increased Lipoprotein-Associated Phospholipase A2 and Low High-Density Lipoprotein-Cholesterol: The Veterans Affairs HDL Intervention Trial Arterioscler. Thromb. Vasc. Biol., June 1, 2008; 28(6): 1172 - 1178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. G. Saougos, A. P. Tambaki, M. Kalogirou, M. Kostapanos, I. F. Gazi, R. L. Wolfert, M. Elisaf, and A. D. Tselepis Differential Effect of Hypolipidemic Drugs on Lipoprotein-Associated Phospholipase A2 Arterioscler. Thromb. Vasc. Biol., October 1, 2007; 27(10): 2236 - 2243. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Zhang, Y. Uehara, S. Hida, S.-i. Miura, D. L. Rainwater, M. Segawa, K. Kumagai, K.-A. Rye, and K. Saku Effects of reconstituted HDL on charge-based LDL subfractions as characterized by capillary isotachophoresis J. Lipid Res., May 1, 2007; 48(5): 1175 - 1189. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. G. Tsouli, D. N. Kiortsis, E. S. Lourida, V. Xydis, L. D. Tsironis, M. I. Argyropoulou, M. Elisaf, and A. D. Tselepis Autoantibody titers against OxLDL are correlated with Achilles tendon thickness in patients with familial hypercholesterolemia J. Lipid Res., October 1, 2006; 47(10): 2208 - 2214. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kontush and M. J. Chapman Functionally Defective High-Density Lipoprotein: A New Therapeutic Target at the Crossroads of Dyslipidemia, Inflammation, and Atherosclerosis Pharmacol. Rev., September 1, 2006; 58(3): 342 - 374. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Koenig, D. Twardella, H. Brenner, and D. Rothenbacher Lipoprotein-Associated Phospholipase A2 Predicts Future Cardiovascular Events in Patients With Coronary Heart Disease Independently of Traditional Risk Factors, Markers of Inflammation, Renal Function, and Hemodynamic Stress Arterioscler. Thromb. Vasc. Biol., July 1, 2006; 26(7): 1586 - 1593. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. C. Papavasiliou, C. Gouva, K. C. Siamopoulos, and A. D. Tselepis PAF-acetylhydrolase activity in plasma of patients with chronic kidney disease. Effect of long-term therapy with erythropoietin Nephrol. Dial. Transplant., May 1, 2006; 21(5): 1270 - 1277. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. H. Yang, J. P. McConnell, R. J. Lennon, G. W. Barsness, G. Pumper, S. J. Hartman, C. S. Rihal, L. O. Lerman, and A. Lerman Lipoprotein-Associated Phospholipase A2 Is an Independent Marker for Coronary Endothelial Dysfunction in Humans Arterioscler. Thromb. Vasc. Biol., January 1, 2006; 26(1): 106 - 111. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Gazi, E. S. Lourida, T. Filippatos, V. Tsimihodimos, M. Elisaf, and A. D. Tselepis Lipoprotein-Associated Phospholipase A2 Activity Is a Marker of Small, Dense LDL Particles in Human Plasma Clin. Chem., December 1, 2005; 51(12): 2264 - 2273. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. N. Kiortsis, S. Tsouli, E. S. Lourida, V. Xydis, M. I. Argyropoulou, M. Elisaf, and A. D. Tselepis Lack of Association Between Carotid Intima-Media Thickness and PAF-Acetylhydrolase Mass and Activity in Patients with Primary Hyperlipidemia Angiology, July 1, 2005; 56(4): 451 - 458. [Abstract] [PDF]
|
|
|
|
|
|
S. Campo, M. A. Sardo, A. Bitto, A. Bonaiuto, G. Trimarchi, M. Bonaiuto, M. Castaldo, C. Saitta, S. Cristadoro, and A. Saitta Platelet-Activating Factor Acetylhydrolase Is Not Associated with Carotid Intima-Media Thickness in Hypercholesterolemic Sicilian Individuals Clin. Chem., November 1, 2004; 50(11): 2077 - 2082. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kontush, E. C. de Faria, S. Chantepie, and M. J. Chapman Antioxidative Activity of HDL Particle Subspecies Is Impaired in Hyperalphalipoproteinemia: Relevance of Enzymatic and Physicochemical Properties Arterioscler. Thromb. Vasc. Biol., March 1, 2004; 24(3): 526 - 533. [Abstract] [Full Text]
|
|
|
|
|
|
T. Kujiraoka, T. Iwasaki, M. Ishihara, M. Ito, M. Nagano, A. Kawaguchi, S. Takahashi, J. Ishi, M. Tsuji, T. Egashira, et al. Altered distribution of plasma PAF-AH between HDLs and other lipoproteins in hyperlipidemia and diabetes mellitus J. Lipid Res., October 1, 2003; 44(10): 2006 - 2014. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kontush, S. Chantepie, and M. J. Chapman Small, Dense HDL Particles Exert Potent Protection of Atherogenic LDL Against Oxidative Stress Arterioscler. Thromb. Vasc. Biol., October 1, 2003; 23(10): 1881 - 1888. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Benitez, J. L. Sanchez-Quesada, V. Ribas, O. Jorba, F. Blanco-Vaca, F. Gonzalez-Sastre, and J. Ordonez-Llanos Platelet-Activating Factor Acetylhydrolase Is Mainly Associated With Electronegative Low-Density Lipoprotein Subfraction Circulation, July 8, 2003; 108(1): 92 - 96. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Blankenberg, D. Stengel, H. J. Rupprecht, C. Bickel, J. Meyer, F. Cambien, L. Tiret, and E. Ninio Plasma PAF-acetylhydrolase in patients with coronary artery disease: results of a cross-sectional analysis J. Lipid Res., July 1, 2003; 44(7): 1381 - 1386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Tsimihodimos, A. Kakafika, A. P. Tambaki, E. Bairaktari, M. J. Chapman, M. Elisaf, and A. D. Tselepis Fenofibrate induces HDL-associated PAF-AH but attenuates enzyme activity associated with apoB-containing lipoproteins J. Lipid Res., May 1, 2003; 44(5): 927 - 934. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
