HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Murdoch, S. J. Articles by Albers, J. J. Search for Related Content PubMed PubMed Citation Articles by Murdoch, S. J. Articles by Albers, J. J. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 43, 281-289, February 2002
Copyright © 2002 by Lipid Research, Inc.

Differences in reactivity of antibodies to active versus inactive PLTP significantly impacts PLTP measurement

Correspondence to: John J. Albers, at Northwest Lipid Research Laboratories, 2121 N. 35th Street, Seattle, WA 98103., jja{at}u.washington.edu (E-mail)

Due to conflicting reports concerning the relationship between phospholipid transfer protein (PLTP) activity and mass in plasma, the protein concentration and activity of PLTP were assessed in fractions isolated by fast protein liquid chromatography from the plasma of healthy normolipidemic individuals. Using both polyclonal and monoclonal antibodies, PLTP was identified by Western blot analysis after both SDS and non-denaturing gradient gel electrophoresis, and quantitated by dot blot. PLTP activity was determined using a labeled vesicle/HDL assay. PLTP mass corresponded substantially with the activity distribution using the polyclonal antibody on dot blot with some inactive PLTP being present. However, the monoclonal antibody preferentially reacted with inactive PLTP, primarily associated with LDL and large HDL, overestimating inactive PLTP. Western blot analysis of non-denaturing gradient gels, using the polyclonal antibody, indicated that active PLTP was associated with numerous discrete HDL subpopulations (7.6–12.0 nm) with the major portion being 9–12 nm. Inactive PLTP was associated with particles of 12 to >17 nm. The monoclonal antibody demonstrated a different pattern of reactivity on gradient gels, showing strong reactivity with the inactive PLTP in particles of 12 to >17 nm, but less reactivity with particles of 7.6–12 nm.

The differences in reactivities of antibodies for active versus inactive PLTP can account for some of the discrepancies reported in the literature regarding the relationship between PLTP mass and activity. — Murdoch, S. J., G. Wolfbauer, H. Kennedy, S. M. Marcovina, M. C. Carr, and J. J. Albers. Differences in reactivity of antibodies to active versus inactive PLTP significantly impacts PLTP measurement. J. Lipid Res. 2002. 43: 281–289.

Supplementary key words: phospholipid transfer protein, high density lipoprotein, apolipoprotein A-I, apolipoprotein E, lipoprotein distribution

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. C. Cheung and J. J. Albers Active plasma phospholipid transfer protein is associated with apoA-I- but not apoE-containing lipoproteins J. Lipid Res., June 1, 2006; 47(6): 1315 - 1321. [Abstract] [Full Text] [PDF]

				G. M. Dallinga-Thie, A. van Tol, H. Hattori, P. C.N. Rensen, E. J.G. Sijbrands, and for the Diabetes Atorvastatin Lipid Intervention ( Plasma phospholipid transfer protein activity is decreased in type 2 diabetes during treatment with atorvastatin: a role for apolipoprotein e? Diabetes, May 1, 2006; 55(5): 1491 - 1496. [Abstract] [Full Text] [PDF]

				M. T. Janis, S. Siggins, E. Tahvanainen, R. Vikstedt, K. Silander, J. Metso, A. Aromaa, M.-R. Taskinen, V. M. Olkkonen, M. Jauhiainen, et al. Active and low-active forms of serum phospholipid transfer protein in a normal Finnish population sample J. Lipid Res., December 1, 2004; 45(12): 2303 - 2309. [Abstract] [Full Text] [PDF]

				A.-Y. Tu, M. C. Cheung, X. Zhu, R. H. Knopp, and J. J. Albers Low-Density Lipoprotein Inhibits Secretion of Phospholipid Transfer Protein in Human Trophoblastic BeWo Cells Experimental Biology and Medicine, November 1, 2004; 229(10): 1046 - 1052. [Abstract] [Full Text] [PDF]

				S. Siggins, M. Karkkainen, J. Tenhunen, J. Metso, E. Tahvanainen, V. M. Olkkonen, M. Jauhiainen, and C. Ehnholm Quantitation of the active and low-active forms of human plasma phospholipid transfer protein by ELISA J. Lipid Res., February 1, 2004; 45(2): 387 - 395. [Abstract] [Full Text] [PDF]

				S. Siggins, M. Jauhiainen, V. M. Olkkonen, J. Tenhunen, and C. Ehnholm PLTP secreted by HepG2 cells resembles the high-activity PLTP form in human plasma J. Lipid Res., September 1, 2003; 44(9): 1698 - 1704. [Abstract] [Full Text] [PDF]

				S. J. Murdoch, S. E. Kahn, J. J. Albers, J. D. Brunzell, and J. Q. Purnell PLTP activity decreases with weight loss: changes in PLTP are associated with changes in subcutaneous fat and FFA but not IAF or insulin sensitivity J. Lipid Res., September 1, 2003; 44(9): 1705 - 1712. [Abstract] [Full Text] [PDF]

				K. D. O'Brien, S. Vuletic, T. O. McDonald, G. Wolfbauer, K. Lewis, A.-Y. Tu, S. Marcovina, T. N. Wight, A. Chait, and J. J. Albers Cell-Associated and Extracellular Phospholipid Transfer Protein in Human Coronary Atherosclerosis Circulation, July 22, 2003; 108(3): 270 - 274. [Abstract] [Full Text] [PDF]

				S. Vuletic, L.-W. Jin, S. M. Marcovina, E. R. Peskind, T. Moller, and J. J. Albers Widespread distribution of PLTP in human CNS: evidence for PLTP synthesis by glia and neurons, and increased levels in Alzheimer's disease J. Lipid Res., June 1, 2003; 44(6): 1113 - 1123. [Abstract] [Full Text] [PDF]

				T. Oka, S. Yamashita, T. Kujiraoka, M. Ito, M. Nagano, Y. Sagehashi, T. Egashira, M. N. Nanjee, K.-i. Hirano, N. E. Miller, et al. Distribution of human plasma PLTP mass and activity in hypo- and hyperalphalipoproteinemia J. Lipid Res., August 1, 2002; 43(8): 1236 - 1243. [Abstract] [Full Text] [PDF]