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Correspondence to:
Silvia Santamarina-Fojo, at Molecular Disease Branch, NHLBI-NIH, Bldg. 10, Room 7N115, 10 Center Dr., Bethesda, MD 20892-1666., silvia{at}mdb.nhlbi.nih.gov (E-mail)
To identify regulatory elements in the proximal human ATP-binding cassette transporter A1 (hABCA1) gene promoter we transfected RAW cells with plasmids containing mutations in the E-box, AP1, and liver X receptor (LXR) elements as well as the two Sp1 motifs. Point mutations in either Sp1 site or in the AP1 site had only a minor effect whereas mutation of the LXR element decreased promoter activity. In contrast, mutation or deletion of the E-box motif caused a 3-fold increase in transcriptional activity under basal conditions. Gel shift and DNaseI footprint analysis showed binding of a protein or protein complex to this region. Preincubation of nuclear extracts with antibodies established that USF1, USF2, and fos related antigen (Fra) 2 bind to DNA sequences in the human ABCA1 promoter that contains the intact E-box but not the mutant or deleted E-box. Co-transfection of USF1 and USF2 enhanced, but Fra2 repressed, ABCA1 promoter activity. Thus, a complex consisting of USF1, USF2, and Fra2 binds the E-box motif 147 bp upstream of the transcriptional start site and facilitates repression of the human ABCA1 promoter.
These combined studies identify a novel site in the human ABCA1 promoter involved in the regulation of ABCA1 gene expression. — Yang, X-P., L. A. Freeman, C. L. Knapper, M. J. A. Amar, A. Remaley, H. B. Brewer, Jr., and S. Santamarina-Fojo. The E-box motif in the proximal ABCA1 promoter mediates transcriptional repression of the ABCA1 gene. J. Lipid Res. 2002. 43: 297–306.
Supplementary key words:
high density lipoproteins, ATP-binding cassette transporter, E-box
Copyright © 2002 by Lipid Research, Inc.
The E-box motif in the proximal ABCA1 promoter mediates transcriptional repression of the ABCA1 gene
Xiao-Ping Yanga,
Lita A. Freemana,
Catherine L. Knappera,
Marcelo J. A. Amara,
Alan Remaleya,
H. Bryan Brewer, Jr.a, and
Silvia Santamarina-Fojoa
a Molecular Disease Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892
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