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Journal of Lipid Research, Vol. 43, 392-397, March 2002
Copyright © 2002 by Lipid Research, Inc.

LCAT facilitates transacylation of 17ß-estradiol in the presence of HDL₃ subfraction

Correspondence to: Matti J. Tikkanen, at the Department of Medicine, Division of Cardiology, Helsinki University Central Hospital, FIN-00290, Helsinki, Finland., matti.tikkanen{at}hus.fi (E-mail)

It has been shown that estrogens need to be metabolized to their hydrophobic estrogen ester derivatives to act as antioxidants in lipoproteins. Data suggest that 17ß-estradiol (E₂) becomes esterified in LCAT-induced reactions and the esters are transported from HDL particles to LDL and VLDL particles by a CETP-dependent mechanism. In the present study we have further investigated the regulation of E₂ esterification by LCAT and focused on the importance of HDL structure and composition in the esterification process. Isolated LDL, HDL₂, HDL₃, and reconstituted discoidal HDL (rHDL) were incubated with labeled E₂, with and without purified LCAT, at 37°C for 24 h. After purification of the lipoprotein fractions, there was a significant peak of radioactivity representing esterified estradiol attached to HDL₃ and rHDL, but HDL₂ and LDL contained only trace amounts of labeled estradiol ester. TLC analysis confirmed that the radioactivity migrated in a position corresponding to that of 17ß-E₂ 17-monoester standard. The amount of radioactivity associated with HDL₃ and rHDL representing esterified E₂ was significantly increased by addition of purified LCAT. However, only limited increases of radioactivity were observed in HDL₂ and LDL.

In conclusion, HDL subfractions differ in their potential to regulate estradiol esterification by LCAT. — Höckerstedt, A., M. J. Tikkanen, and M. Jauhiainen. LCAT facilitates transacylation of 17ß-estradiol in the presence of HDL₃ subfraction. J. Lipid Res. 2002. 43: 392–397.

Supplementary key words: estrogen, estradiol ester, lecithin:cholesterol acyltransferase, high density lipoprotein 2, high density lipoprotein 3, reconstituted discoidal HDL

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