| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Correspondence to: William S. Garver, To whom correspondence should be addressed., wgarver{at}peds.arizona.edu (E-mail)
The Niemann-Pick C1 (NPC1) protein regulates cholesterol transport from late endosomes-lysosomes to other intracellular compartments. In this article, cholesterol transport to caveolin-1 and caveolin-2 containing compartments, such as the trans-Golgi network (TGN) and plasma membrane caveolae, was examined in normal (NPC+/+), NPC heterozygous (NPC+/-), and NPC homozygous (NPC-/-) human fibroblasts. The expression and distribution of NPC1 in each cell type were similar, and characterized by a finely dispersed, granular staining pattern. The expression of caveolin-1 and caveolin-2 was increased in NPC+/- and NPC-/- fibroblasts, although the distribution in each cell type was similar and characterized by predominant staining of the TGN and plasma membrane. The TGN in NPC+/+ fibroblasts was relatively cholesterol-enriched, whereas the TGN in NPC+/- and NPC-/- fibroblasts was partially or completely cholesterol-deficient, respectively. Consistent with studies demonstrating the transport of cholesterol from the TGN to plasma membrane caveolae, the concentration of cholesterol in plasma membrane caveolae isolated from NPC+/- and NPC-/- fibroblasts was significantly decreased, even though the total concentration of plasma membrane cholesterol in each cell type was similar.
These studies demonstrate that NPC1 regulates cholesterol transport to caveolin-1 and caveolin-2 containing compartments such as the TGN and plasma membrane caveolae.—Garver, W. S., K. Krishnan, J. R. Gallagos, M. Michikawa, G. A. Francis, and R. A. Heidenreich. Niemann-Pick C1 protein regulates cholesterol transport to the trans-Golgi network and plasma membrane caveolae. J. Lipid Res. 2002. 43: 579–589.
Supplementary key words: caveolin-1, caveolin-2, fibroblasts
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  W. S. Garver, D. Jelinek, G. A. Francis, and B. D. Murphy The Niemann-Pick C1 gene is downregulated by feedback inhibition of the SREBP pathway in human fibroblasts J. Lipid Res., May 1, 2008; 49(5): 1090 - 1102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Yamauchi, P. C. Reid, J. B. Sperry, K. Furukawa, M. Takeya, C. C. Y. Chang, and T.-Y. Chang Plasma Membrane Rafts Complete Cholesterol Synthesis by Participating in Retrograde Movement of Precursor Sterols J. Biol. Chem., November 30, 2007; 282(48): 34994 - 35004. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. M. White, D. Jiang, J. D. Burgess, I. R. Bederman, S. F. Previs, and T. J. Kelley Altered cholesterol homeostasis in cultured and in vivo models of cystic fibrosis Am J Physiol Lung Cell Mol Physiol, February 1, 2007; 292(2): L476 - L486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Boadu, H. Y. Choi, D. W. K. Lee, E. I. Waddington, T. Chan, B. Asztalos, J. E. Vance, A. Chan, G. Castro, and G. A. Francis Correction of Apolipoprotein A-I-mediated Lipid Efflux and High Density Lipoprotein Particle Formation in Human Niemann-Pick Type C Disease Fibroblasts J. Biol. Chem., December 1, 2006; 281(48): 37081 - 37090. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Chinetti-Gbaguidi, E. Rigamonti, L. Helin, A. L. Mutka, M. Lepore, J. C. Fruchart, V. Clavey, E. Ikonen, S. Lestavel, and B. Staels Peroxisome proliferator-activated receptor {alpha} controls cellular cholesterol trafficking in macrophages J. Lipid Res., December 1, 2005; 46(12): 2717 - 2725. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Riff, J. W. Callahan, and P. M. Sherman Cholesterol-Enriched Membrane Microdomains Are Required for Inducing Host Cell Cytoskeleton Rearrangements in Response to Attaching-Effacing Escherichia coli Infect. Immun., November 1, 2005; 73(11): 7113 - 7125. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Rigamonti, L. Helin, S. Lestavel, A.L. Mutka, M. Lepore, C. Fontaine, M.A. Bouhlel, S. Bultel, J.C. Fruchart, E. Ikonen, et al. Liver X Receptor Activation Controls Intracellular Cholesterol Trafficking and Esterification in Human Macrophages Circ. Res., September 30, 2005; 97(7): 682 - 689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. S. Garver, C. Xie, J. J. Repa, S. D. Turley, and J. M. Dietschy Niemann-Pick C1 expression is not regulated by the amount of cholesterol flowing through cells in the mouse J. Lipid Res., August 1, 2005; 46(8): 1745 - 1754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Yu, M. Ko, K. Yanagisawa, and M. Michikawa Neurodegeneration in Heterozygous Niemann-Pick Type C1 (NPC1) Mouse: IMPLICATION OF HETEROZYGOUS NPC1 MUTATIONS BEING A RISK FOR TAUOPATHY J. Biol. Chem., July 22, 2005; 280(29): 27296 - 27302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Yu, J.-S. Gong, M. Ko, W. S. Garver, K. Yanagisawa, and M. Michikawa Altered Cholesterol Metabolism in Niemann-Pick Type C1 Mouse Brains Affects Mitochondrial Function J. Biol. Chem., March 25, 2005; 280(12): 11731 - 11739. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Y. Choi, B. Karten, T. Chan, J. E. Vance, W. L. Greer, R. A. Heidenreich, W. S. Garver, and G. A. Francis Impaired ABCA1-dependent Lipid Efflux and Hypoalphalipoproteinemia in Human Niemann-Pick type C Disease J. Biol. Chem., August 29, 2003; 278(35): 32569 - 32577. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. C. Reid, S. Sugii, and T.-Y. Chang Trafficking defects in endogenously synthesized cholesterol in fibroblasts, macrophages, hepatocytes, and glial cells from Niemann-Pick type C1 mice J. Lipid Res., May 1, 2003; 44(5): 1010 - 1019. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. S. Blom, M. D. Linder, K. Snow, H. Pihko, M. W. Hess, E. Jokitalo, V. Veckman, A.-C. Syvanen, and E. Ikonen Defective endocytic trafficking of NPC1 and NPC2 underlying infantile Niemann-Pick type C disease Hum. Mol. Genet., February 1, 2003; 12(3): 257 - 272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Walter, J. P. Davies, and Y. A. Ioannou Telomerase immortalization upregulates Rab9 expression and restores LDL cholesterol egress from Niemann-Pick C1 late endosomes J. Lipid Res., February 1, 2003; 44(2): 243 - 253. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
