HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Masuno, H. Articles by Honda, K. Search for Related Content PubMed PubMed Citation Articles by Masuno, H. Articles by Honda, K. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 43, 676-684, May 2002
Copyright © 2002 by Lipid Research, Inc.

Bisphenol A in combination with insulin can accelerate the conversion of 3T3-L1 fibroblasts to adipocytes

Hiroshi Masuno^1,*, Teruki Kidani, Keizo Sekiya, Kenshi Sakayama, Takahiko Shiosaka^*, Haruyasu Yamamoto and Katsuhisa Honda^**

^* Department of Medical Laboratory Technology, Ehime College of Health Science, Takooda, Tobe-cho, Iyo-gun, Ehime 791-2101, Japan
Department of Orthopedic Surgery, School of Medicine, Ehime University, Shigenobu, Onsen-gun, Ehime 791-0295, Japan
National Agricultural Research Center for Western Region, Zentsuji, Kagawa 765-8508, Japan
^** Department of Environmental Science for Industry, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, Japan

¹ To whom correspondence should be addressed. e-mail: hmasuno{at}ehime-chs.ac.jp

The confluent cultures of 3T3-L1 fibroblasts were treated with or without bisphenol A (BPA) for 2 days and subsequently treated with insulin (INS) alone for 9 days. When BPA was absent during the first 2-day treatment period, the cultures contained 1.6 µg/µg DNA of triacylglycerol (TG), 202 mU/mg DNA of lipoprotein lipase (LPL) activity, and 462 nmol/min/mg DNA of glycerol-3-phosphate dehydrogenase (GPDH) activity. The presence of BPA during the same period caused a 150% increase in the TG content, a 60% increase in the LPL activity, and a 500% increase in the GPDH activity. Thus, BPA by itself can trigger 3T3-L1 fibroblasts to differentiate into adipocytes. Next, the confluent cultures were treated with BPA for 2 days and subsequently treated with a combination of INS and BPA for 9 days. The simultaneous presence of BPA with INS caused a 370% increase in the TG content, a 200% increase in the LPL activity, and a 225% increase in the GPDH activity compared with the cultures treated with INS alone. The amount of [³H]thymidine incorporated into DNA was lower in the cultures treated with INS in the presence of BPA than in those treated with INS alone, indicating that BPA has an anti-proliferative activity on 3T3-L1 cells. Taken together, our results indicate that BPA in combination with INS can accelerate the adipocyte conversion.—Masuno, H., T. Kidani, K. Sekiya, K. Sakayama, T. Shiosaka, H. Yamamoto, and K. Honda. Bisphenol A in combination with insulin can accelerate the conversion of 3T3-L1 fibroblasts to adipocytes. J. Lipid Res. 2002. 43: 676–684.

Abbreviations: BPA, bisphenol A; BPADA, bisphenol A diacetate; BPABCF, bisphenol A bis(chloroformate); BPADGE, bisphenol A diglycidyl ether; DEX, dexamethasone; GPDH, glycerol-3-phosphate dehydrogenase; INS, insulin; LPL, lipoprotein lipase; MIX, 3-isobutyl-1-methylxanthine; TG, triacylglycerol

Supplementary key words environmental endocrine disrupting chemical • lipoprotein lipase • glycerol-3-phosphate dehydrogenase • triacylglycerol accumulation • 3T3-L1 adipocytes

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				L. N. Vandenberg, M. V. Maffini, P. R. Wadia, C. Sonnenschein, B. S. Rubin, and A. M. Soto Exposure to Environmentally Relevant Doses of the Xenoestrogen Bisphenol-A Alters Development of the Fetal Mouse Mammary Gland Endocrinology, January 1, 2007; 148(1): 116 - 127. [Abstract] [Full Text] [PDF]

				F. Grun, H. Watanabe, Z. Zamanian, L. Maeda, K. Arima, R. Cubacha, D. M. Gardiner, J. Kanno, T. Iguchi, and B. Blumberg Endocrine-Disrupting Organotin Compounds Are Potent Inducers of Adipogenesis in Vertebrates Mol. Endocrinol., September 1, 2006; 20(9): 2141 - 2155. [Abstract] [Full Text] [PDF]

				F. Grun and B. Blumberg Environmental Obesogens: Organotins and Endocrine Disruption via Nuclear Receptor Signaling Endocrinology, June 1, 2006; 147(6): s50 - s55. [Abstract] [Full Text] [PDF]

				H. Masuno, J. Iwanami, T. Kidani, K. Sakayama, and K. Honda Bisphenol A Accelerates Terminal Differentiation of 3T3-L1 Cells into Adipocytes through the Phosphatidylinositol 3-Kinase Pathway Toxicol. Sci., April 1, 2005; 84(2): 319 - 327. [Abstract] [Full Text] [PDF]

				J. J. Heindel Endocrine Disruptors and the Obesity Epidemic Toxicol. Sci., December 1, 2003; 76(2): 247 - 249. [Full Text] [PDF]

				H. Masuno, S. Okamoto, J. Iwanami, K. Honda, T. Shiosaka, T. Kidani, K. Sakayama, and H. Yamamoto Effect of 4-Nonylphenol on Cell Proliferation and Adipocyte Formation in Cultures of Fully Differentiated 3T3-L1 Cells Toxicol. Sci., October 1, 2003; 75(2): 314 - 320. [Abstract] [Full Text] [PDF]