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* Lipids and Cardiovascular Epidemiology Unit, Institut Municipal d'Investigació Mèdica (IMIM)
Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra
Servei de Cardiologia, Hospital del Mar
** Laboratori de Referència, Barcelona, Spain
1 To whom correspondence should be addressed at Lipids and Cardiovascular Epidemiology Unit, Institut Municipal d'Investigació Mèdica (IMIM), Dr Aiguader 80, E-08003 Barcelona, Spain. e-mail: mtomas{at}imim.es
Regular exercise practise is a protective factor against coronary heart disease and enhances antioxidant systems, whereas acute exercise appears to be a major source of increased oxidative stress. Paraoxonase1 (PON1) is an antioxidant HDL-linked enzyme, whose activity toward paraoxon (PON1 activity) is strongly modulated by the PON1-192 polymorphism, comprising Q and R alleles for low and high PON1 activity, respectively. Another polymorphism at the PON1 locus, the PON1-55, modulates PON1 protein and activity levels. PON1 activity, lipid levels, and oxidized LDL concentration were determined in 17 healthy young volunteers before and after a 16-weeks aerobic exercise training period. Furthermore, PON1 activity was analyzed after a bout of exercise in both situations. We found that regular exercise was associated with a decrease in oxidized LDL levels, and an increase in PON1 activity in QQ subjects and with a decrease in PON1 activity in R carriers. A bout of exercise produced an increase in PON1 activity just after the bout of exercise, followed by a decrease in its activity. A recovery of the basal PON1 activity levels at 24 h was found in QQ subjects regardless of their training status and in trained R carriers, but not in untrained R carriers. These results suggest that the effects of regular and acute exercise on PON1 activity levels are modulated by PON1-192 polymorphism. Changes were less evident for the PON1-55 polymorphism.Tomás, M., R. Elosua, M. Sentí, L. Molina, J. Vila, R. Anglada, M. Fitó, M. I. Covas, J. Marrugat. PON1-192 polymorphism modulates the effects of regular and acute exercise on paraoxonase1 activity. J. Lipid Res. 2002. 43: 713720.
Abbreviations: APO, aerobic power output; BMI, body mass index; CHD, coronary heart disease; HDL-C, serum HDL-cholesterol; LDL-C, serum LDL-cholesterol; MANOVA, multivariate analysis of variance; MPO, maximum power output; PA, physical activity; PON1, paraoxonase1; PON1 activity, Paraoxonase1 activity towards paraoxon; RER, respiratory-exchange ratio; TBARS, thiobarbituric acid reactive substances method; TC, serum total cholesterol; TG, serum triglycerides; VO2, oxygen uptake; VO2max, maximal oxygen uptake; VT, ventilatory anaerobic threshold
Supplementary key words bout of exercise exercise training PON1 genotypes oxidative stress
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