HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Eurlings, P. M. H. Articles by de Bruin, T. W. A. Search for Related Content PubMed PubMed Citation Articles by Eurlings, P. M. H. Articles by de Bruin, T. W. A. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 43, 930-935, June 2002
Copyright © 2002 by Lipid Research, Inc.

Identification of differentially expressed genes in subcutaneous adipose tissue from subjects with familial combined hyperlipidemia

Petra M. H. Eurlings^*, Carla J. H. van der Kallen^*, Jan M. W. Geurts^*, Paul Kouwenberg, Willy D. Boeckx and Tjerk W. A. de Bruin^1,*

^* Cardiovascular Research Institute Maastricht, Laboratory of Molecular Metabolism and Endocrinology, Department of Internal Medicine, University of Maastricht, Maastricht, The Netherlands
Department of Plastic Surgery, Academic Hospital Maastricht, The Netherlands

¹ To whom correspondence should be addressed. e-mail: tdb{at}sint.azm.nl

Subjects with familial combined hyperlipidemia (FCHL) are characterized by a complex metabolic phenotype with hyperlipidemia, insulin resistance, and central obesity. FCHL is due to impaired adipose tissue function superimposed on hepatic overproduction of lipoproteins. We investigated adipose tissue as an interesting target tissue for differential gene expression in FCHL. Human cDNA expression array analyses, in which adipose tissue from five FCHL patients was compared with that from four age, gender, and BMI matched controls, resulted in the identification of 22 up-regulated and three down-regulated genes. The genes differentially expressed imply activation of the adipocyte cell cycle genes. Furthermore, the differential expression of the genes coding for tumor necrosis factor , interleukin 6, and intracellular adhesion molecule 1 support a role for adipose tissue in insulin resistance in FCHL subjects.

The observed changes represent a primary genetic defect, an adaptive response, or a contribution of both.

Abbreviations: apo, apolipoprotein; BMI, body mass index; CAD, coronary artery disease; CCDN1, G1/S-specific cyclin D1; CCDN2, G1/S-specific cyclin D2; CDKN1A, cyclin-dependent kinase inhibitor 1A; ERBB3, v-erb-b2 erythroblastic leukemia viral oncogen homolog 3; FCHL, familial combined hyperlipidemia; GADD45, DNA damage-inducible gene GADD45; ICAM1, intracellular adhesion molecule 1; ID1, DNA binding protein inhibitor ID1; IFNGR2, interferon gamma receptor 2; IL-6, interleukin 6; KRBH, Krebs-Ringer bicarbonate; TC, total cholesterol; TG, triglycerides; TNF, tumor necrosis factor alpha

Supplementary key words atlas human cDNA expression array • TNF • IL-6 • cell cycle genes

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M De Michele, A Iannuzzi, A Salvato, P Pauciullo, M Gentile, G Iannuzzo, S Panico, A Pujia, G M Bond, and P Rubba Impaired endothelium-dependent vascular reactivity in patients with familial combined hyperlipidaemia Heart, January 1, 2007; 93(1): 78 - 81. [Abstract] [Full Text] [PDF]

				G. M. van der Vleuten, L. J. H. van Tits, M. den Heijer, H. Lemmers, A. F. H. Stalenhoef, and J. de Graaf Decreased adiponectin levels in familial combined hyperlipidemia patients contribute to the atherogenic lipid profile J. Lipid Res., November 1, 2005; 46(11): 2398 - 2404. [Abstract] [Full Text] [PDF]

				L. Khaodhiar, P.-R. Ling, G. L. Blackburn, and B. R. Bistrian Serum Levels of Interleukin-6 and C-Reactive Protein Correlate With Body Mass Index Across the Broad Range of Obesity JPEN J Parenter Enteral Nutr, November 1, 2004; 28(6): 410 - 415. [Abstract] [Full Text] [PDF]

				F. Morello, T. W.A. de Bruin, J. I. Rotter, R. E. Pratt, C. J.H. van der Kallen, G. A. Hladik, V. J. Dzau, C.-C. Liew, and Y.-D. I. Chen Differential Gene Expression of Blood-Derived Cell Lines in Familial Combined Hyperlipidemia Arterioscler. Thromb. Vasc. Biol., November 1, 2004; 24(11): 2149 - 2154. [Abstract] [Full Text] [PDF]

				M. Ridderstrale, E. Carlsson, M. Klannemark, A. Cederberg, C. Kosters, H. Tornqvist, H. Storgaard, A. Vaag, S. Enerback, and L. Groop FOXC2 mRNA Expression and a 5' Untranslated Region Polymorphism of the Gene Are Associated With Insulin Resistance Diabetes, December 1, 2002; 51(12): 3554 - 3560. [Abstract] [Full Text] [PDF]

				M. M. J. van Greevenbroek, V. M. M-J. Vermeulen, and T. W. A. de Bruin Familial combined hyperlipidemia plasma stimulates protein secretion by HepG2 cells: identification of fibronectin in the differential secretion proteome J. Lipid Res., November 1, 2002; 43(11): 1846 - 1854. [Abstract] [Full Text] [PDF]